safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	33	48.10

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
EKBFFMEE_62	60320	61189	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
EKBFFMEE_58	231	1646	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
EKBFFMEE_34	705	1685	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
EKBFFMEE_34	1678	2862	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
EKBFFMEE_34	2879	3931	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
EKBFFMEE_34	3946	4854	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
EKBFFMEE_34	4875	5936	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
EKBFFMEE_34	5973	6464	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
EKBFFMEE_34	6476	6952	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
EKBFFMEE_34	9132	9872	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
EKBFFMEE_34	9882	10574	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
EKBFFMEE_34	10583	11314	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
EKBFFMEE_34	11315	12775	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
EKBFFMEE_62	61189	61782	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
EKBFFMEE_12	10825	12570	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
EKBFFMEE_12	12572	14272	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
EKBFFMEE_12	14290	15492	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
EKBFFMEE_12	15492	16298	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
EKBFFMEE_12	16298	17239	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
EKBFFMEE_12	17236	18384	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
EKBFFMEE_12	18503	19279	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
EKBFFMEE_12	19625	20341	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
EKBFFMEE_12	20342	21310	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
EKBFFMEE_12	21328	22305	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
EKBFFMEE_12	22302	23561	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
EKBFFMEE_34	7708	9075	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
EKBFFMEE_62	61189	61782	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
EKBFFMEE_12	12572	14272	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
EKBFFMEE_62	61189	61782	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
EKBFFMEE_25	2393	4051	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
EKBFFMEE_12	15492	16298	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
EKBFFMEE_12	16298	17239	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
EKBFFMEE_12	14290	15492	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
EKBFFMEE_12	17236	18384	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
EKBFFMEE_16	2124	3092	cbpD	Autolysin	Exoenzyme	98.86	100.00	WP_082302295
EKBFFMEE_34	85907	86697	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
EKBFFMEE_34	9137	9872	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
EKBFFMEE_34	9132	9872	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
EKBFFMEE_34	10583	11314	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
EKBFFMEE_12	10825	12570	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
EKBFFMEE_34	10583	11314	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
EKBFFMEE_62	25659	26420	srtA	Sortase A	Exoenzyme	97.64	100.00	-
EKBFFMEE_34	11315	12775	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
EKBFFMEE_34	11315	12775	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
EKBFFMEE_12	23680	24531	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
EKBFFMEE_34	7712	9075	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
EKBFFMEE_34	9882	10574	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
EKBFFMEE_34	9882	10574	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
EKBFFMEE_23	19344	20647	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
EKBFFMEE_32	13387	14397	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
EKBFFMEE_12	15492	16298	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
EKBFFMEE_68	59407	60690	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
EKBFFMEE_62	62211	63257	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
EKBFFMEE_12	14755	15492	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EKBFFMEE_62	22972	21989	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
EKBFFMEE_19	13975	12902	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
EKBFFMEE_53	63435	61132	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
EKBFFMEE_4	15173	16141	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
EKBFFMEE_10	33601	35082	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
EKBFFMEE_24	11077	13323	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
EKBFFMEE_17	1941	943	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
EKBFFMEE_29	15275	14319	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.60	98.46	ABF36968
EKBFFMEE_12	72669	71518	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
EKBFFMEE_12	37570	35582	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
EKBFFMEE_62	39171	40901	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
EKBFFMEE_6	30385	28907	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
EKBFFMEE_23	19344	20645	Eno	0.0	enolase	86.21	99.77	ABP90468
EKBFFMEE_6	15650	13758	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
EKBFFMEE_24	16248	14539	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
EKBFFMEE_2	40149	39139	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
EKBFFMEE_45	22054	20693	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
EKBFFMEE_30	10285	8393	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
EKBFFMEE_23	4908	5798	bglB	2.70e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
EKBFFMEE_8	4804	3866	SP_0676	1.89e-169	transcription facot	81.47	97.20	AAK74821
EKBFFMEE_24	13515	14372	metF	1.47e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
EKBFFMEE_34	49598	48894	covR	2.07e-132	responder protein	85.53	100.00	AAK71319
EKBFFMEE_10	8994	8323	rr01	6.51e-126	response regulator	83.93	99.56	CAB54566
EKBFFMEE_34	49598	48894	walR	8.72e-123	DNA-binding response regulator	82.13	100.00	ANC99504
EKBFFMEE_50	1892	2560	CiaR	7.31e-122	two-component response regulator	84.31	99.55	AAK74935
EKBFFMEE_50	1892	2563	ciaR	9.62e-120	two component system response regulator	82.14	99.12	AIK75708
EKBFFMEE_14	24031	24717	CovR	1.70e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
EKBFFMEE_17	667	293	SpxA2	1.34e-71	transcriptional regulator	91.20	94.70	ERL21461
EKBFFMEE_45	33417	33818	SpxA1	1.77e-67	transcriptional regulator	80.60	100.00	ERL19273
EKBFFMEE_13	13030	13317	spr0479	2.95e-49	essential gene	86.46	98.97	AAK99283
EKBFFMEE_11	6517	6263	spr1327	4.16e-45	essential gene	94.12	100.00	AAL00131
EKBFFMEE_33	1215	952	spr0175	5.34e-43	pathogenitcity related	84.09	100.00	AAK98979
EKBFFMEE_33	388	122	spr0177	1.16e-34	essential gene	84.27	88.12	AAK98981
EKBFFMEE_68	59249	58749	RopE (M5005_Spy_1611)	1.74e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
EKBFFMEE_53	63435	61138	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
EKBFFMEE_2	4288	5307	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EKBFFMEE_2	0.0	1.0	0.0
EKBFFMEE_3	0.0	1.0	0.0
EKBFFMEE_4	0.0	1.0	0.0
EKBFFMEE_5	0.0	1.0	0.0
EKBFFMEE_6	0.0	1.0	0.0
EKBFFMEE_7	0.0	1.0	0.0
EKBFFMEE_8	0.0	1.0	0.0
EKBFFMEE_9	0.0	1.0	0.0
EKBFFMEE_10	0.0	1.0	0.0
EKBFFMEE_11	0.0	1.0	0.0
EKBFFMEE_12	0.0	1.0	0.0
EKBFFMEE_13	0.0	1.0	0.0
EKBFFMEE_14	0.0	1.0	0.0
EKBFFMEE_15	0.0	1.0	0.0
EKBFFMEE_16	0.0	1.0	0.0
EKBFFMEE_17	0.0	1.0	0.0
EKBFFMEE_18	0.0	1.0	0.0
EKBFFMEE_19	0.0	1.0	0.0
EKBFFMEE_20	0.0	1.0	0.0
EKBFFMEE_21	0.0	1.0	0.0
EKBFFMEE_22	0.0	1.0	0.0
EKBFFMEE_23	0.0	1.0	0.0
EKBFFMEE_24	0.0	1.0	0.0
EKBFFMEE_25	0.0	1.0	0.0
EKBFFMEE_26	0.0	1.0	0.0
EKBFFMEE_27	0.0	1.0	0.0
EKBFFMEE_28	0.0	1.0	0.0
EKBFFMEE_29	0.0	1.0	0.0
EKBFFMEE_30	0.0	1.0	0.0
EKBFFMEE_31	0.0	1.0	0.0
EKBFFMEE_32	0.0	1.0	0.0
EKBFFMEE_33	0.0	1.0	0.0
EKBFFMEE_34	0.0	1.0	0.0
EKBFFMEE_35	0.0	1.0	0.0
EKBFFMEE_36	0.0	1.0	0.0
EKBFFMEE_37	0.0	1.0	0.0
EKBFFMEE_38	0.0	1.0	0.0
EKBFFMEE_39	0.0	1.0	0.0
EKBFFMEE_40	0.0	1.0	0.0
EKBFFMEE_41	0.0	1.0	0.0
EKBFFMEE_42	0.0	1.0	0.0
EKBFFMEE_43	0.0	1.0	0.0
EKBFFMEE_44	0.0	1.0	0.0
EKBFFMEE_45	0.0	1.0	0.0
EKBFFMEE_46	0.0	1.0	0.0
EKBFFMEE_47	0.0	1.0	0.0
EKBFFMEE_48	0.0	1.0	0.0
EKBFFMEE_49	0.0	1.0	0.0
EKBFFMEE_50	0.0	1.0	0.0
EKBFFMEE_51	0.0	1.0	0.0
EKBFFMEE_52	1.0	0.0	1.0
EKBFFMEE_53	0.0	1.0	0.0
EKBFFMEE_54	0.0	1.0	0.0
EKBFFMEE_55	1.0	0.0	1.0
EKBFFMEE_56	1.0	0.0	1.0
EKBFFMEE_57	0.0	1.0	0.0
EKBFFMEE_58	1.0	0.0	1.0
EKBFFMEE_59	0.0	1.0	0.0
EKBFFMEE_60	1.0	0.0	1.0
EKBFFMEE_62	0.0	1.0	0.0
EKBFFMEE_68	0.0	1.0	0.0
EKBFFMEE_76	0.0	1.0	0.0
EKBFFMEE_81	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found