Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 24 | 33 | 40.80 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NONAKEBI_18 | 19439 | 20308 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| NONAKEBI_18 | 20308 | 20901 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| NONAKEBI_4 | 16628 | 17437 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| NONAKEBI_18 | 20308 | 20901 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NONAKEBI_4 | 17421 | 18377 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| NONAKEBI_4 | 18374 | 19522 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| NONAKEBI_18 | 20308 | 20901 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| NONAKEBI_4 | 23018 | 23869 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| NONAKEBI_8 | 25146 | 26804 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| NONAKEBI_4 | 15423 | 16628 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| NONAKEBI_4 | 12567 | 14351 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226325 |
| NONAKEBI_4 | 19631 | 20641 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| NONAKEBI_4 | 14348 | 15403 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.34 | 100.00 | WP_002948022 |
| NONAKEBI_7 | 14576 | 15337 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| NONAKEBI_12 | 16472 | 17311 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.69 | 100.00 | WP_011226733 |
| NONAKEBI_20 | 18709 | 19497 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.48 | 99.75 | - |
| NONAKEBI_42 | 13485 | 14216 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| NONAKEBI_42 | 13485 | 14216 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011681176 |
| NONAKEBI_42 | 13485 | 14216 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011227248 |
| NONAKEBI_4 | 20644 | 21915 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| NONAKEBI_42 | 12024 | 13484 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 100.00 | WP_011681177 |
| NONAKEBI_42 | 14927 | 15667 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011681174 |
| NONAKEBI_42 | 14927 | 15667 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_011226046 |
| NONAKEBI_42 | 12024 | 13484 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_011226049 |
| NONAKEBI_42 | 12024 | 13484 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| NONAKEBI_42 | 14927 | 15662 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.06 | 98.13 | WP_011227246 |
| NONAKEBI_42 | 15724 | 17087 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.53 | 99.71 | WP_011226045 |
| NONAKEBI_42 | 14225 | 14917 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| NONAKEBI_42 | 14225 | 14917 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| NONAKEBI_42 | 15724 | 17091 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.52 | 99.93 | WP_011681173 |
| NONAKEBI_42 | 14225 | 14917 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| NONAKEBI_31 | 95612 | 96915 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| NONAKEBI_33 | 3678 | 4688 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| NONAKEBI_1 | 4821 | 6104 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| NONAKEBI_18 | 21330 | 22376 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NONAKEBI_7 | 18024 | 19007 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NONAKEBI_19 | 13764 | 12691 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NONAKEBI_11 | 15527 | 13224 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| NONAKEBI_31 | 11977 | 12945 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| NONAKEBI_22 | 51703 | 53184 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| NONAKEBI_2 | 44036 | 41790 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| NONAKEBI_12 | 36102 | 37094 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| NONAKEBI_12 | 1 | 957 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| NONAKEBI_11 | 129220 | 130371 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| NONAKEBI_4 | 38839 | 36851 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| NONAKEBI_7 | 1829 | 99 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| NONAKEBI_48 | 26986 | 28464 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| NONAKEBI_31 | 95612 | 96913 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NONAKEBI_48 | 42175 | 44067 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| NONAKEBI_27 | 2038 | 329 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| NONAKEBI_53 | 60823 | 59813 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NONAKEBI_42 | 94770 | 96131 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| NONAKEBI_53 | 10456 | 12348 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| NONAKEBI_31 | 81176 | 82066 | bglB | 3.55e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| NONAKEBI_3 | 12340 | 11405 | SP_0676 | 2.04e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| NONAKEBI_2 | 41598 | 40741 | metF | 4.70e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NONAKEBI_2 | 24417 | 23713 | covR | 1.35e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| NONAKEBI_22 | 32728 | 32057 | rr01 | 2.10e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| NONAKEBI_2 | 24417 | 23713 | walR | 5.68e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| NONAKEBI_27 | 5992 | 6660 | CiaR | 8.45e-120 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| NONAKEBI_56 | 66103 | 66789 | CovR | 4.06e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NONAKEBI_27 | 5992 | 6663 | ciaR | 8.31e-118 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| NONAKEBI_12 | 37391 | 37786 | SpxA2 | 7.55e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| NONAKEBI_42 | 83407 | 83006 | SpxA1 | 1.95e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NONAKEBI_9 | 25380 | 25093 | spr0479 | 2.98e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| NONAKEBI_59 | 5641 | 5387 | spr1327 | 7.36e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| NONAKEBI_48 | 75818 | 75555 | spr0175 | 3.29e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| NONAKEBI_48 | 74991 | 74725 | spr0177 | 7.19e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| NONAKEBI_1 | 6262 | 6762 | RopE (M5005_Spy_1611) | 1.75e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NONAKEBI_11 | 15527 | 13230 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| NONAKEBI_53 | 24912 | 25931 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NONAKEBI_1 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_2 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_3 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_4 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_5 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_6 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_7 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_8 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_9 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_10 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_11 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_12 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_13 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_14 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_15 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_16 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_17 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_18 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_19 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_20 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_21 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_22 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_23 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_24 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_25 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_26 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_27 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_28 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_29 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_30 | 1.0 | 0.0 | 1.0 |
| NONAKEBI_31 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_32 | 1.0 | 0.0 | 1.0 |
| NONAKEBI_33 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_34 | 1.0 | 0.0 | 1.0 |
| NONAKEBI_35 | 1.0 | 0.0 | 1.0 |
| NONAKEBI_36 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_37 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_38 | 1.0 | 0.0 | 1.0 |
| NONAKEBI_42 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_48 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_53 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_56 | 0.0 | 1.0 | 0.0 |
| NONAKEBI_59 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)