Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AOKEDNFC_59 | 60259 | 61128 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| AOKEDNFC_59 | 61128 | 61721 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| AOKEDNFC_50 | 137534 | 138340 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681437 |
| AOKEDNFC_59 | 61128 | 61721 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| AOKEDNFC_32 | 13357 | 14088 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.73 | 100.00 | WP_011681176 |
| AOKEDNFC_50 | 141304 | 143049 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_011227426 |
| AOKEDNFC_50 | 136593 | 137534 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| AOKEDNFC_59 | 61128 | 61721 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| AOKEDNFC_47 | 2261 | 3404 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.65 | 100.00 | - |
| AOKEDNFC_50 | 139560 | 141200 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 99.63 | 96.47 | WP_041827753 |
| AOKEDNFC_3 | 12975 | 14633 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| AOKEDNFC_50 | 136593 | 137534 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| AOKEDNFC_50 | 138340 | 139542 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011681436 |
| AOKEDNFC_50 | 137534 | 138340 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| AOKEDNFC_50 | 139560 | 141200 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 99.45 | 96.47 | WP_041827077 |
| AOKEDNFC_50 | 138340 | 139542 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011227428 |
| AOKEDNFC_50 | 131513 | 132787 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | WP_011226331 |
| AOKEDNFC_50 | 132787 | 133749 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.86 | 95.25 | WP_011226330 |
| AOKEDNFC_32 | 14799 | 15539 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| AOKEDNFC_32 | 14799 | 15539 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.38 | 100.00 | WP_011226046 |
| AOKEDNFC_50 | 141304 | 143049 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 98.17 | 100.00 | WP_011681434 |
| AOKEDNFC_50 | 129559 | 130410 | rfbD | 0.0 | Capsule | Immune modulation | 98.12 | 100.00 | WP_014621820 |
| AOKEDNFC_32 | 14799 | 15534 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.10 | 98.13 | WP_011227246 |
| AOKEDNFC_32 | 13357 | 14088 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.95 | 100.00 | WP_011227248 |
| AOKEDNFC_32 | 13357 | 14088 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011226048 |
| AOKEDNFC_59 | 25599 | 26360 | srtA | 0.0 | Sortase A | Exoenzyme | 97.77 | 100.00 | - |
| AOKEDNFC_32 | 11896 | 13356 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| AOKEDNFC_57 | 72964 | 73923 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| AOKEDNFC_32 | 11896 | 13356 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.51 | 100.21 | WP_011226049 |
| AOKEDNFC_32 | 11896 | 13356 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.69 | 100.21 | WP_041827043 |
| AOKEDNFC_32 | 15595 | 16960 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.39 | 99.85 | WP_011681173 |
| AOKEDNFC_32 | 15595 | 16962 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.37 | 100.00 | WP_011226045 |
| AOKEDNFC_50 | 135451 | 136596 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 93.02 | 99.74 | WP_011227431 |
| AOKEDNFC_50 | 135451 | 136596 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 92.76 | 99.74 | WP_011681439 |
| AOKEDNFC_32 | 14097 | 14789 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.50 | 100.00 | WP_011681175 |
| AOKEDNFC_12 | 78984 | 80287 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| AOKEDNFC_32 | 14097 | 14789 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 87.88 | 100.00 | WP_011226047 |
| AOKEDNFC_32 | 14097 | 14789 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 87.59 | 100.00 | WP_011227247 |
| AOKEDNFC_37 | 51 | 1061 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| AOKEDNFC_50 | 137534 | 138340 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| AOKEDNFC_1 | 6252 | 7535 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| AOKEDNFC_59 | 62150 | 63196 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| AOKEDNFC_32 | 25910 | 26493 | SPD_RS01775 | 1.87e-148 | Capsule | Immune modulation | 83.11 | 96.04 | WP_001819836 |
| AOKEDNFC_50 | 138340 | 139077 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.96 | 60.29 | WP_012000441 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AOKEDNFC_11 | 27641 | 28714 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| AOKEDNFC_59 | 22912 | 21929 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.21 | 100.00 | AAK75326 |
| AOKEDNFC_40 | 40723 | 43026 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| AOKEDNFC_12 | 162531 | 161563 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| AOKEDNFC_61 | 56484 | 57965 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.71 | 100.00 | ABJ54480 |
| AOKEDNFC_36 | 6284 | 8530 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| AOKEDNFC_9 | 1986 | 988 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| AOKEDNFC_9 | 39321 | 38350 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| AOKEDNFC_50 | 80424 | 81575 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| AOKEDNFC_50 | 116383 | 118371 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| AOKEDNFC_59 | 39111 | 40841 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| AOKEDNFC_55 | 73127 | 71649 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| AOKEDNFC_12 | 80287 | 78986 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| AOKEDNFC_55 | 58389 | 56497 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| AOKEDNFC_36 | 11455 | 9746 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| AOKEDNFC_2 | 54828 | 53818 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| AOKEDNFC_3 | 39610 | 38249 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| AOKEDNFC_2 | 4493 | 6385 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| AOKEDNFC_12 | 94722 | 93832 | bglB | 4.95e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| AOKEDNFC_7 | 11508 | 10573 | SP_0676 | 2.01e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| AOKEDNFC_36 | 8722 | 9579 | metF | 1.41e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| AOKEDNFC_54 | 24384 | 23680 | covR | 2.53e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| AOKEDNFC_61 | 28380 | 27709 | rr01 | 1.12e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| AOKEDNFC_54 | 24384 | 23680 | walR | 1.07e-122 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| AOKEDNFC_35 | 7670 | 6984 | CovR | 1.50e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| AOKEDNFC_36 | 15409 | 16077 | CiaR | 1.87e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| AOKEDNFC_36 | 15409 | 16080 | ciaR | 1.84e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| AOKEDNFC_9 | 712 | 338 | SpxA2 | 2.70e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| AOKEDNFC_3 | 51075 | 51476 | SpxA1 | 1.32e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| AOKEDNFC_15 | 13648 | 13935 | spr0479 | 2.98e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| AOKEDNFC_57 | 24984 | 24730 | spr1327 | 9.62e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| AOKEDNFC_55 | 13799 | 14062 | spr0175 | 3.86e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| AOKEDNFC_55 | 14626 | 14892 | spr0177 | 8.43e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| AOKEDNFC_1 | 7693 | 8193 | RopE (M5005_Spy_1611) | 1.81e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| AOKEDNFC_40 | 40723 | 43020 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| AOKEDNFC_2 | 18948 | 19967 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AOKEDNFC_1 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_2 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_3 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_4 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_5 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_6 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_7 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_9 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_10 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_11 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_12 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_15 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_25 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_32 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_33 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_34 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_35 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_36 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_37 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_38 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_39 | 1.0 | 0.0 | 1.0 |
| AOKEDNFC_40 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_41 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_42 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_43 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_44 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_45 | 1.0 | 0.0 | 1.0 |
| AOKEDNFC_46 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_47 | 1.0 | 0.0 | 1.0 |
| AOKEDNFC_48 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_49 | 1.0 | 0.0 | 1.0 |
| AOKEDNFC_50 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_51 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_54 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_55 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_57 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_58 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_59 | 0.0 | 1.0 | 0.0 |
| AOKEDNFC_61 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AOKEDNFC_5 | 42420 | 52816 | True | Siphoviridae | VOG9667, VOG4603, VOG4602, VOG9667, VOG9403, VOG0275, VOG0058, VOG0944, VOG5532 |
Phigaro v2.4.0 (Database: Jan. 2024 release)