Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 34 | 40.50 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PCFHLFBB_6 | 25913 | 27172 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| PCFHLFBB_27 | 60399 | 60992 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| PCFHLFBB_13 | 20641 | 21480 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.88 | 100.00 | WP_011226733 |
| PCFHLFBB_27 | 60399 | 60992 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| PCFHLFBB_27 | 60399 | 60992 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| PCFHLFBB_6 | 27169 | 28145 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| PCFHLFBB_18 | 277 | 1935 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| PCFHLFBB_6 | 28163 | 29131 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| PCFHLFBB_27 | 24872 | 25633 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| PCFHLFBB_27 | 59530 | 60399 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| PCFHLFBB_27 | 105023 | 105813 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| PCFHLFBB_3 | 16382 | 17113 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| PCFHLFBB_3 | 17114 | 18574 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.49 | 100.00 | WP_011681177 |
| PCFHLFBB_6 | 29133 | 29848 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| PCFHLFBB_6 | 30194 | 30964 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 98.18 | 99.23 | WP_011681440 |
| PCFHLFBB_3 | 16382 | 17113 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011226048 |
| PCFHLFBB_3 | 16382 | 17113 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.54 | 100.00 | WP_011681176 |
| PCFHLFBB_3 | 17114 | 18574 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.47 | 100.21 | WP_011226049 |
| PCFHLFBB_6 | 24943 | 25794 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| PCFHLFBB_11 | 9863 | 14758 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.89 | 100.00 | WP_000167699 |
| PCFHLFBB_3 | 14931 | 15671 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.76 | 100.00 | WP_011681174 |
| PCFHLFBB_3 | 17114 | 18574 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_041827043 |
| PCFHLFBB_3 | 14931 | 15671 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.49 | 100.00 | WP_011226046 |
| PCFHLFBB_3 | 14936 | 15671 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.20 | 98.13 | WP_011227246 |
| PCFHLFBB_3 | 15681 | 16373 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011227247 |
| PCFHLFBB_3 | 15681 | 16373 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.81 | 100.00 | WP_011226047 |
| PCFHLFBB_11 | 9863 | 14806 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.81 | 99.82 | YP_001200536 |
| PCFHLFBB_11 | 9863 | 14806 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.79 | 99.82 | YP_001198331 |
| PCFHLFBB_3 | 13507 | 14874 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.61 | 100.00 | WP_011226045 |
| PCFHLFBB_3 | 13509 | 14874 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.29 | 99.85 | WP_011681173 |
| PCFHLFBB_3 | 15681 | 16373 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| PCFHLFBB_11 | 48197 | 49500 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| PCFHLFBB_17 | 14294 | 15304 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| PCFHLFBB_2 | 62896 | 64179 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| PCFHLFBB_27 | 61421 | 62467 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PCFHLFBB_11 | 14779 | 9866 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.40 | 99.70 | AUS90958 |
| PCFHLFBB_27 | 22185 | 21202 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| PCFHLFBB_14 | 13657 | 12584 | MntE | 0.0 | manganese homeostasis | 94.13 | 99.72 | ABF32167 |
| PCFHLFBB_11 | 133056 | 132088 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| PCFHLFBB_21 | 153977 | 156280 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| PCFHLFBB_34 | 65714 | 64233 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| PCFHLFBB_16 | 11291 | 9045 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| PCFHLFBB_13 | 1850 | 852 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| PCFHLFBB_15 | 15255 | 14284 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| PCFHLFBB_21 | 24112 | 22961 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| PCFHLFBB_6 | 11904 | 13892 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| PCFHLFBB_27 | 38381 | 40111 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| PCFHLFBB_44 | 62688 | 61210 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| PCFHLFBB_11 | 49500 | 48199 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| PCFHLFBB_44 | 47465 | 45573 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| PCFHLFBB_16 | 6120 | 7829 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| PCFHLFBB_3 | 49821 | 50831 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| PCFHLFBB_46 | 62346 | 63707 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| PCFHLFBB_8 | 33217 | 31325 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| PCFHLFBB_11 | 63929 | 63039 | bglB | 5.14e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| PCFHLFBB_4 | 41447 | 42385 | SP_0676 | 9.91e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| PCFHLFBB_16 | 8853 | 7996 | metF | 7.81e-167 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| PCFHLFBB_27 | 133454 | 134158 | covR | 5.75e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| PCFHLFBB_34 | 93321 | 93992 | rr01 | 3.06e-126 | response regulator | 84.38 | 99.56 | CAB54566 |
| PCFHLFBB_27 | 133454 | 134158 | walR | 2.72e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| PCFHLFBB_43 | 84025 | 84711 | CovR | 5.08e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| PCFHLFBB_16 | 2166 | 1498 | CiaR | 6.47e-119 | two-component response regulator | 83.86 | 99.55 | AAK74935 |
| PCFHLFBB_16 | 2166 | 1495 | ciaR | 6.43e-117 | two component system response regulator | 81.70 | 99.12 | AIK75708 |
| PCFHLFBB_13 | 576 | 181 | SpxA2 | 4.29e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| PCFHLFBB_46 | 51189 | 50788 | SpxA1 | 1.75e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| PCFHLFBB_7 | 25405 | 25118 | spr0479 | 3.33e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| PCFHLFBB_11 | 167474 | 167220 | spr1327 | 2.61e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| PCFHLFBB_44 | 13857 | 14120 | spr0175 | 4.46e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| PCFHLFBB_44 | 14660 | 14950 | spr0177 | 2.62e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| PCFHLFBB_2 | 62738 | 62238 | RopE (M5005_Spy_1611) | 3.66e-76 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| PCFHLFBB_21 | 153977 | 156274 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| PCFHLFBB_8 | 18760 | 17741 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PCFHLFBB_1 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_2 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_3 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_4 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_5 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_6 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_7 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_8 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_9 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_10 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_11 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_12 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_13 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_14 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_15 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_16 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_17 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_18 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_19 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_20 | 1.0 | 0.0 | 1.0 |
| PCFHLFBB_21 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_22 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_23 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_24 | 1.0 | 0.0 | 1.0 |
| PCFHLFBB_25 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_26 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_27 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_34 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_38 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_41 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_43 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_44 | 0.0 | 1.0 | 0.0 |
| PCFHLFBB_46 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| PCFHLFBB_10 | 6938 | 9176 | False | Unknown | VOG6396, VOG6396, VOG9405 |
Phigaro v2.4.0 (Database: Jan. 2024 release)