Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EEECPGCG_12 | 186926 | 187735 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| EEECPGCG_12 | 185986 | 186942 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| EEECPGCG_12 | 184841 | 185989 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| EEECPGCG_12 | 180494 | 181345 | rfbD | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_014621820 |
| EEECPGCG_6 | 13057 | 14715 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| EEECPGCG_12 | 187735 | 188940 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226326 |
| EEECPGCG_12 | 190012 | 191796 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226325 |
| EEECPGCG_12 | 183722 | 184732 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226330 |
| EEECPGCG_12 | 188960 | 190015 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.34 | 100.00 | WP_002948022 |
| EEECPGCG_29 | 2465 | 3880 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_011681165 |
| EEECPGCG_5 | 14576 | 15337 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| EEECPGCG_16 | 20652 | 21491 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.69 | 100.00 | WP_011226733 |
| EEECPGCG_17 | 772 | 1560 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.48 | 99.75 | - |
| EEECPGCG_9 | 13485 | 14216 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| EEECPGCG_9 | 13485 | 14216 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011681176 |
| EEECPGCG_9 | 13485 | 14216 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.68 | 100.00 | WP_011227248 |
| EEECPGCG_12 | 182448 | 183719 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 97.48 | 99.76 | WP_011226331 |
| EEECPGCG_9 | 12024 | 13484 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 100.00 | WP_011681177 |
| EEECPGCG_9 | 14927 | 15667 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011681174 |
| EEECPGCG_9 | 14927 | 15667 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 96.36 | 100.00 | WP_011226046 |
| EEECPGCG_9 | 12024 | 13484 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.30 | 100.21 | WP_011226049 |
| EEECPGCG_9 | 12024 | 13484 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| EEECPGCG_9 | 14927 | 15662 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.06 | 98.13 | WP_011227246 |
| EEECPGCG_9 | 15724 | 17087 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.53 | 99.71 | WP_011226045 |
| EEECPGCG_9 | 14225 | 14917 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011227247 |
| EEECPGCG_9 | 14225 | 14917 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.40 | 99.86 | WP_011226047 |
| EEECPGCG_9 | 15724 | 17091 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.52 | 99.93 | WP_011681173 |
| EEECPGCG_9 | 14225 | 14917 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.47 | 100.00 | WP_011681175 |
| EEECPGCG_24 | 77737 | 79040 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| EEECPGCG_28 | 3678 | 4688 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.46 | 99.80 | WP_002986042 |
| EEECPGCG_2 | 4821 | 6104 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EEECPGCG_5 | 18024 | 19007 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EEECPGCG_47 | 24889 | 25962 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| EEECPGCG_12 | 15527 | 13224 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EEECPGCG_24 | 162675 | 161707 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| EEECPGCG_35 | 51988 | 53469 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| EEECPGCG_47 | 53267 | 55513 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EEECPGCG_16 | 1861 | 869 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| EEECPGCG_21 | 15348 | 14392 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| EEECPGCG_12 | 129220 | 130371 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| EEECPGCG_12 | 165524 | 167512 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| EEECPGCG_5 | 1829 | 99 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| EEECPGCG_50 | 63264 | 61786 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| EEECPGCG_24 | 79040 | 77739 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| EEECPGCG_50 | 48075 | 46183 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| EEECPGCG_26 | 2006 | 297 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| EEECPGCG_43 | 21548 | 22558 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EEECPGCG_3 | 61252 | 62613 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EEECPGCG_43 | 71915 | 70023 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| EEECPGCG_24 | 93476 | 92586 | bglB | 4.94e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| EEECPGCG_4 | 11674 | 10739 | SP_0676 | 2.02e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| EEECPGCG_47 | 55705 | 56562 | metF | 7.99e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| EEECPGCG_47 | 72886 | 73590 | covR | 2.29e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| EEECPGCG_35 | 33013 | 32342 | rr01 | 2.12e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| EEECPGCG_47 | 72886 | 73590 | walR | 9.64e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EEECPGCG_26 | 5960 | 6628 | CiaR | 8.41e-120 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| EEECPGCG_52 | 73615 | 74301 | CovR | 4.48e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| EEECPGCG_26 | 5960 | 6631 | ciaR | 8.27e-118 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| EEECPGCG_16 | 572 | 177 | SpxA2 | 4.30e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| EEECPGCG_3 | 49889 | 49488 | SpxA1 | 1.27e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| EEECPGCG_8 | 25380 | 25093 | spr0479 | 2.98e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| EEECPGCG_45 | 99735 | 99989 | spr1327 | 1.09e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| EEECPGCG_50 | 14432 | 14695 | spr0175 | 3.48e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| EEECPGCG_50 | 15259 | 15525 | spr0177 | 7.60e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| EEECPGCG_2 | 6262 | 6762 | RopE (M5005_Spy_1611) | 1.74e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EEECPGCG_12 | 15527 | 13230 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EEECPGCG_43 | 57459 | 56440 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EEECPGCG_2 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_3 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_4 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_5 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_6 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_8 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_9 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_10 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_11 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_12 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_13 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_15 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_16 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_17 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_18 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_20 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_21 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_22 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_23 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_24 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_26 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_27 | 1.0 | 0.0 | 1.0 |
| EEECPGCG_28 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_29 | 1.0 | 0.0 | 1.0 |
| EEECPGCG_30 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_31 | 1.0 | 0.0 | 1.0 |
| EEECPGCG_33 | 1.0 | 0.0 | 1.0 |
| EEECPGCG_34 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_35 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_36 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_38 | 1.0 | 0.0 | 1.0 |
| EEECPGCG_41 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_43 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_45 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_46 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_47 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_50 | 0.0 | 1.0 | 0.0 |
| EEECPGCG_52 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)