safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	32	47.42

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MEBJBEIB_55	60320	61189	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
MEBJBEIB_47	231	1646	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
MEBJBEIB_54	86364	87344	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
MEBJBEIB_54	85187	86371	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
MEBJBEIB_54	84118	85170	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
MEBJBEIB_54	83195	84103	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
MEBJBEIB_54	82113	83174	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
MEBJBEIB_54	81585	82076	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
MEBJBEIB_54	81097	81573	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
MEBJBEIB_54	78177	78917	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
MEBJBEIB_54	77475	78167	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
MEBJBEIB_54	76735	77466	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
MEBJBEIB_54	75274	76734	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
MEBJBEIB_55	61189	61782	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
MEBJBEIB_44	18392	20137	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
MEBJBEIB_44	20139	21839	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
MEBJBEIB_44	21857	23059	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
MEBJBEIB_44	23059	23865	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
MEBJBEIB_44	23865	24806	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
MEBJBEIB_44	24803	25951	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
MEBJBEIB_44	26070	26846	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
MEBJBEIB_44	27192	27908	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
MEBJBEIB_44	27909	28877	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
MEBJBEIB_44	28895	29872	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
MEBJBEIB_44	29869	31128	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
MEBJBEIB_54	78974	80341	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
MEBJBEIB_55	61189	61782	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
MEBJBEIB_44	20139	21839	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
MEBJBEIB_55	61189	61782	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
MEBJBEIB_32	89717	91375	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
MEBJBEIB_44	23059	23865	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
MEBJBEIB_44	23865	24806	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
MEBJBEIB_44	21857	23059	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
MEBJBEIB_44	24803	25951	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
MEBJBEIB_17	18867	19835	cbpD	Autolysin	Exoenzyme	98.86	100.00	WP_082302295
MEBJBEIB_54	1352	2142	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
MEBJBEIB_54	78177	78912	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
MEBJBEIB_54	78177	78917	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
MEBJBEIB_54	76735	77466	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
MEBJBEIB_44	18392	20137	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
MEBJBEIB_54	76735	77466	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
MEBJBEIB_55	25659	26420	srtA	Sortase A	Exoenzyme	97.64	100.00	-
MEBJBEIB_54	75274	76734	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
MEBJBEIB_54	75274	76734	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
MEBJBEIB_44	31247	32098	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
MEBJBEIB_54	78974	80337	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
MEBJBEIB_54	77475	78167	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
MEBJBEIB_54	77475	78167	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
MEBJBEIB_12	75059	76362	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
MEBJBEIB_24	14294	15304	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
MEBJBEIB_44	23059	23865	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
MEBJBEIB_1	2215	3498	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
MEBJBEIB_55	62211	63257	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
MEBJBEIB_44	22322	23059	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MEBJBEIB_55	22972	21989	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
MEBJBEIB_10	13975	12902	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
MEBJBEIB_22	63435	61132	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
MEBJBEIB_5	37294	36326	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
MEBJBEIB_60	53024	54505	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
MEBJBEIB_16	16954	19200	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
MEBJBEIB_18	1863	865	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
MEBJBEIB_19	4177	5148	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
MEBJBEIB_44	80236	79085	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
MEBJBEIB_44	45137	43149	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
MEBJBEIB_55	39171	40901	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
MEBJBEIB_3	13825	15303	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
MEBJBEIB_12	76362	75061	Eno	0.0	enolase	86.21	99.77	ABP90468
MEBJBEIB_3	28560	30452	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
MEBJBEIB_16	22125	20416	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
MEBJBEIB_2	22967	23977	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
MEBJBEIB_32	65282	66643	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
MEBJBEIB_12	90798	89908	bglB	3.47e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
MEBJBEIB_6	39362	40300	SP_0676	2.25e-169	transcription facot	81.47	97.20	AAK74821
MEBJBEIB_16	19392	20249	metF	2.30e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
MEBJBEIB_54	38451	39155	covR	2.06e-132	responder protein	85.53	100.00	AAK71319
MEBJBEIB_60	28417	27746	rr01	1.06e-125	response regulator	83.93	99.56	CAB54566
MEBJBEIB_54	38451	39155	walR	8.68e-123	DNA-binding response regulator	82.13	100.00	ANC99504
MEBJBEIB_14	7669	6983	CovR	1.71e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
MEBJBEIB_16	26080	26748	CiaR	3.06e-119	two-component response regulator	84.31	99.55	AAK74935
MEBJBEIB_16	26080	26751	ciaR	3.01e-117	two component system response regulator	82.14	99.12	AIK75708
MEBJBEIB_18	589	215	SpxA2	1.39e-71	transcriptional regulator	91.20	94.70	ERL21461
MEBJBEIB_32	53919	53518	SpxA1	2.12e-67	transcriptional regulator	80.60	100.00	ERL19273
MEBJBEIB_13	25411	25124	spr0479	2.93e-49	essential gene	86.46	98.97	AAK99283
MEBJBEIB_11	33675	33929	spr1327	4.16e-45	essential gene	94.12	100.00	AAL00131
MEBJBEIB_20	1215	952	spr0175	6.41e-43	pathogenitcity related	84.09	100.00	AAK98979
MEBJBEIB_20	388	122	spr0177	1.40e-34	essential gene	84.27	88.12	AAK98981
MEBJBEIB_1	3656	4156	RopE (M5005_Spy_1611)	1.68e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
MEBJBEIB_22	63435	61138	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
MEBJBEIB_2	58828	57809	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MEBJBEIB_1	0.0	1.0	0.0
MEBJBEIB_2	0.0	1.0	0.0
MEBJBEIB_3	0.0	1.0	0.0
MEBJBEIB_4	0.0	1.0	0.0
MEBJBEIB_5	0.0	1.0	0.0
MEBJBEIB_6	0.0	1.0	0.0
MEBJBEIB_7	0.0	1.0	0.0
MEBJBEIB_8	0.0	1.0	0.0
MEBJBEIB_10	0.0	1.0	0.0
MEBJBEIB_11	0.0	1.0	0.0
MEBJBEIB_12	0.0	1.0	0.0
MEBJBEIB_13	0.0	1.0	0.0
MEBJBEIB_14	0.0	1.0	0.0
MEBJBEIB_15	0.0	1.0	0.0
MEBJBEIB_16	0.0	1.0	0.0
MEBJBEIB_17	0.0	1.0	0.0
MEBJBEIB_18	0.0	1.0	0.0
MEBJBEIB_19	0.0	1.0	0.0
MEBJBEIB_20	0.0	1.0	0.0
MEBJBEIB_21	0.0	1.0	0.0
MEBJBEIB_22	0.0	1.0	0.0
MEBJBEIB_23	0.0	1.0	0.0
MEBJBEIB_24	0.0	1.0	0.0
MEBJBEIB_25	0.0	1.0	0.0
MEBJBEIB_26	0.0	1.0	0.0
MEBJBEIB_27	0.0	1.0	0.0
MEBJBEIB_28	0.0	1.0	0.0
MEBJBEIB_29	0.0	1.0	0.0
MEBJBEIB_30	0.0	1.0	0.0
MEBJBEIB_31	0.0	1.0	0.0
MEBJBEIB_32	0.0	1.0	0.0
MEBJBEIB_33	0.0	1.0	0.0
MEBJBEIB_34	0.0	1.0	0.0
MEBJBEIB_36	0.0	1.0	0.0
MEBJBEIB_37	1.0	0.0	1.0
MEBJBEIB_38	0.0	1.0	0.0
MEBJBEIB_39	0.0	1.0	0.0
MEBJBEIB_42	1.0	0.0	1.0
MEBJBEIB_43	1.0	0.0	1.0
MEBJBEIB_44	0.0	1.0	0.0
MEBJBEIB_45	0.0	1.0	0.0
MEBJBEIB_46	0.0	1.0	0.0
MEBJBEIB_47	1.0	0.0	1.0
MEBJBEIB_49	0.0	1.0	0.0
MEBJBEIB_50	0.0	1.0	0.0
MEBJBEIB_54	0.0	1.0	0.0
MEBJBEIB_55	0.0	1.0	0.0
MEBJBEIB_57	0.0	1.0	0.0
MEBJBEIB_60	0.0	1.0	0.0
MEBJBEIB_62	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found