safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	32	47.42

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IGOBICIB_64	60320	61189	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
IGOBICIB_58	231	1646	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
IGOBICIB_52	705	1685	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
IGOBICIB_52	1678	2862	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
IGOBICIB_52	2879	3931	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
IGOBICIB_52	3946	4854	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
IGOBICIB_52	4875	5936	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
IGOBICIB_52	5973	6464	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
IGOBICIB_52	6476	6952	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
IGOBICIB_52	9132	9872	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
IGOBICIB_52	9882	10574	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
IGOBICIB_52	10583	11314	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
IGOBICIB_52	11315	12775	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
IGOBICIB_64	61189	61782	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
IGOBICIB_15	104907	106652	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
IGOBICIB_15	103205	104905	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
IGOBICIB_15	101985	103187	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
IGOBICIB_15	101179	101985	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
IGOBICIB_15	100238	101179	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
IGOBICIB_15	99093	100241	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
IGOBICIB_15	98198	98974	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
IGOBICIB_15	97136	97852	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
IGOBICIB_15	96167	97135	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
IGOBICIB_15	95172	96149	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
IGOBICIB_15	93916	95175	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
IGOBICIB_52	7708	9075	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
IGOBICIB_64	61189	61782	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
IGOBICIB_15	103205	104905	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
IGOBICIB_64	61189	61782	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
IGOBICIB_32	13069	14727	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
IGOBICIB_15	101179	101985	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
IGOBICIB_15	100238	101179	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
IGOBICIB_15	101985	103187	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
IGOBICIB_15	99093	100241	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
IGOBICIB_21	18867	19835	cbpD	Autolysin	Exoenzyme	98.86	100.00	WP_082302295
IGOBICIB_52	85907	86697	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
IGOBICIB_52	9137	9872	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
IGOBICIB_52	9132	9872	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
IGOBICIB_52	10583	11314	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
IGOBICIB_15	104907	106652	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
IGOBICIB_52	10583	11314	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
IGOBICIB_64	25659	26420	srtA	Sortase A	Exoenzyme	97.64	100.00	-
IGOBICIB_52	11315	12775	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
IGOBICIB_52	11315	12775	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
IGOBICIB_15	92946	93797	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
IGOBICIB_52	7712	9075	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
IGOBICIB_52	9882	10574	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
IGOBICIB_52	9882	10574	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
IGOBICIB_9	126	1429	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
IGOBICIB_35	14294	15304	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
IGOBICIB_15	101179	101985	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
IGOBICIB_69	2215	3498	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
IGOBICIB_64	62211	63257	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
IGOBICIB_15	101985	102722	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IGOBICIB_64	22972	21989	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IGOBICIB_13	13975	12902	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IGOBICIB_43	40710	43013	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IGOBICIB_7	37290	36322	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
IGOBICIB_66	12910	11429	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
IGOBICIB_20	17204	19450	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IGOBICIB_22	1863	865	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
IGOBICIB_25	4177	5148	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IGOBICIB_15	44940	46091	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
IGOBICIB_15	79907	81895	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
IGOBICIB_64	39171	40901	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
IGOBICIB_5	13824	15302	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IGOBICIB_9	1429	128	Eno	0.0	enolase	86.21	99.77	ABP90468
IGOBICIB_5	28559	30451	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
IGOBICIB_20	22375	20666	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
IGOBICIB_4	22675	23685	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IGOBICIB_32	39162	37801	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
IGOBICIB_9	15865	14975	bglB	1.34e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
IGOBICIB_15	119330	118392	SP_0676	7.01e-169	transcription facot	81.47	97.20	AAK74821
IGOBICIB_20	19642	20499	metF	2.32e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IGOBICIB_52	49598	48894	covR	2.07e-132	responder protein	85.53	100.00	AAK71319
IGOBICIB_66	37517	38188	rr01	1.06e-125	response regulator	83.93	99.56	CAB54566
IGOBICIB_52	49598	48894	walR	8.72e-123	DNA-binding response regulator	82.13	100.00	ANC99504
IGOBICIB_19	7737	7051	CovR	1.71e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IGOBICIB_20	26330	26998	CiaR	3.08e-119	two-component response regulator	84.31	99.55	AAK74935
IGOBICIB_20	26330	27001	ciaR	3.04e-117	two component system response regulator	82.14	99.12	AIK75708
IGOBICIB_22	589	215	SpxA2	1.34e-71	transcriptional regulator	91.20	94.70	ERL21461
IGOBICIB_32	50525	50926	SpxA1	2.12e-67	transcriptional regulator	80.60	100.00	ERL19273
IGOBICIB_18	25411	25124	spr0479	2.93e-49	essential gene	86.46	98.97	AAK99283
IGOBICIB_14	6517	6263	spr1327	4.16e-45	essential gene	94.12	100.00	AAL00131
IGOBICIB_31	1215	952	spr0175	6.41e-43	pathogenitcity related	84.09	100.00	AAK98979
IGOBICIB_31	388	122	spr0177	1.40e-34	essential gene	84.27	88.12	AAK98981
IGOBICIB_69	3656	4156	RopE (M5005_Spy_1611)	1.68e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
IGOBICIB_43	40710	43007	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IGOBICIB_4	58536	57517	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IGOBICIB_3	0.0	1.0	0.0
IGOBICIB_4	0.0	1.0	0.0
IGOBICIB_5	0.0	1.0	0.0
IGOBICIB_7	0.0	1.0	0.0
IGOBICIB_9	0.0	1.0	0.0
IGOBICIB_10	0.0	1.0	0.0
IGOBICIB_11	0.0	1.0	0.0
IGOBICIB_12	0.0	1.0	0.0
IGOBICIB_13	0.0	1.0	0.0
IGOBICIB_14	0.0	1.0	0.0
IGOBICIB_15	0.0	1.0	0.0
IGOBICIB_17	0.0	1.0	0.0
IGOBICIB_18	0.0	1.0	0.0
IGOBICIB_19	0.0	1.0	0.0
IGOBICIB_20	0.0	1.0	0.0
IGOBICIB_21	0.0	1.0	0.0
IGOBICIB_22	0.0	1.0	0.0
IGOBICIB_25	0.0	1.0	0.0
IGOBICIB_27	0.0	1.0	0.0
IGOBICIB_29	0.0	1.0	0.0
IGOBICIB_31	0.0	1.0	0.0
IGOBICIB_32	0.0	1.0	0.0
IGOBICIB_33	0.0	1.0	0.0
IGOBICIB_34	0.0	1.0	0.0
IGOBICIB_35	0.0	1.0	0.0
IGOBICIB_36	0.0	1.0	0.0
IGOBICIB_37	0.0	1.0	0.0
IGOBICIB_38	0.0	1.0	0.0
IGOBICIB_39	0.0	1.0	0.0
IGOBICIB_40	0.0	1.0	0.0
IGOBICIB_41	0.0	1.0	0.0
IGOBICIB_42	0.0	1.0	0.0
IGOBICIB_43	0.0	1.0	0.0
IGOBICIB_44	0.0	1.0	0.0
IGOBICIB_45	0.0	1.0	0.0
IGOBICIB_46	0.0	1.0	0.0
IGOBICIB_48	0.0	1.0	0.0
IGOBICIB_49	0.0	1.0	0.0
IGOBICIB_50	1.0	0.0	1.0
IGOBICIB_51	0.0	1.0	0.0
IGOBICIB_52	0.0	1.0	0.0
IGOBICIB_53	1.0	0.0	1.0
IGOBICIB_54	1.0	0.0	1.0
IGOBICIB_55	0.0	1.0	0.0
IGOBICIB_56	1.0	0.0	1.0
IGOBICIB_57	0.0	1.0	0.0
IGOBICIB_58	1.0	0.0	1.0
IGOBICIB_59	0.0	1.0	0.0
IGOBICIB_60	1.0	0.0	1.0
IGOBICIB_61	0.0	1.0	0.0
IGOBICIB_64	0.0	1.0	0.0
IGOBICIB_65	0.0	1.0	0.0
IGOBICIB_66	0.0	1.0	0.0
IGOBICIB_69	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found