safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	33	48.10

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
IDKLJMEJ_53	9521	10390	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
IDKLJMEJ_47	231	1646	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
IDKLJMEJ_50	86313	87293	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
IDKLJMEJ_50	85136	86320	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
IDKLJMEJ_50	84067	85119	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
IDKLJMEJ_50	83144	84052	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
IDKLJMEJ_50	82062	83123	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
IDKLJMEJ_50	81534	82025	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
IDKLJMEJ_50	81046	81522	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
IDKLJMEJ_50	78126	78866	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
IDKLJMEJ_50	77424	78116	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
IDKLJMEJ_50	76684	77415	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
IDKLJMEJ_50	75223	76683	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
IDKLJMEJ_53	8928	9521	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
IDKLJMEJ_33	43918	45663	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
IDKLJMEJ_33	42216	43916	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
IDKLJMEJ_33	40996	42198	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
IDKLJMEJ_33	40190	40996	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
IDKLJMEJ_33	39249	40190	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
IDKLJMEJ_33	38104	39252	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
IDKLJMEJ_33	37209	37985	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
IDKLJMEJ_33	36147	36863	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
IDKLJMEJ_33	35178	36146	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
IDKLJMEJ_33	34183	35160	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
IDKLJMEJ_33	32927	34186	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
IDKLJMEJ_50	78923	80290	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
IDKLJMEJ_53	8928	9521	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
IDKLJMEJ_33	42216	43916	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
IDKLJMEJ_53	8928	9521	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
IDKLJMEJ_20	2393	4051	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
IDKLJMEJ_33	40190	40996	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
IDKLJMEJ_33	39249	40190	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
IDKLJMEJ_33	40996	42198	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
IDKLJMEJ_33	38104	39252	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
IDKLJMEJ_8	34181	35149	cbpD	Autolysin	Exoenzyme	98.86	100.00	WP_082302295
IDKLJMEJ_50	1301	2091	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
IDKLJMEJ_50	78126	78861	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
IDKLJMEJ_50	78126	78866	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
IDKLJMEJ_50	76684	77415	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
IDKLJMEJ_33	43918	45663	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
IDKLJMEJ_50	76684	77415	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
IDKLJMEJ_53	44290	45051	srtA	Sortase A	Exoenzyme	97.64	100.00	-
IDKLJMEJ_50	75223	76683	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
IDKLJMEJ_50	75223	76683	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
IDKLJMEJ_33	31957	32808	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
IDKLJMEJ_50	78923	80286	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
IDKLJMEJ_50	77424	78116	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
IDKLJMEJ_50	77424	78116	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
IDKLJMEJ_11	75060	76363	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
IDKLJMEJ_25	14294	15304	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
IDKLJMEJ_33	40190	40996	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
IDKLJMEJ_64	59407	60690	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
IDKLJMEJ_53	7453	8499	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
IDKLJMEJ_33	40996	41733	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IDKLJMEJ_53	47738	48721	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IDKLJMEJ_10	27677	28750	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IDKLJMEJ_22	57528	59831	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IDKLJMEJ_5	18760	19728	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
IDKLJMEJ_60	53024	54505	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
IDKLJMEJ_41	80996	83242	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IDKLJMEJ_9	40292	41290	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
IDKLJMEJ_9	4177	5148	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IDKLJMEJ_2	44939	46090	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
IDKLJMEJ_33	18918	20906	uvrB	0.0	excinuclease ABC, B subunit	89.14	100.00	AE014259_2
IDKLJMEJ_53	31539	29809	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
IDKLJMEJ_3	13824	15302	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IDKLJMEJ_11	76363	75062	Eno	0.0	enolase	86.21	99.77	ABP90468
IDKLJMEJ_3	28559	30451	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
IDKLJMEJ_41	86167	84458	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
IDKLJMEJ_1	40149	39139	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IDKLJMEJ_41	47712	49073	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
IDKLJMEJ_18	10286	8394	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
IDKLJMEJ_11	90799	89909	bglB	3.47e-179	putative phospho--glucosidase	88.55	99.66	AAK33487
IDKLJMEJ_33	58341	57403	SP_0676	4.30e-169	transcription facot	81.47	97.20	AAK74821
IDKLJMEJ_41	83434	84291	metF	7.59e-166	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IDKLJMEJ_50	38400	39104	covR	2.07e-132	responder protein	85.53	100.00	AAK71319
IDKLJMEJ_60	28417	27746	rr01	1.06e-125	response regulator	83.93	99.56	CAB54566
IDKLJMEJ_50	38400	39104	walR	8.72e-123	DNA-binding response regulator	82.13	100.00	ANC99504
IDKLJMEJ_12	23741	24427	CovR	2.22e-119	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IDKLJMEJ_41	90122	90790	CiaR	1.00e-118	two-component response regulator	84.31	99.55	AAK74935
IDKLJMEJ_41	90122	90793	ciaR	9.88e-117	two component system response regulator	82.14	99.12	AIK75708
IDKLJMEJ_9	41566	41940	SpxA2	2.61e-71	transcriptional regulator	91.20	94.70	ERL21461
IDKLJMEJ_41	36349	35948	SpxA1	1.87e-67	transcriptional regulator	80.60	100.00	ERL19273
IDKLJMEJ_14	25411	25124	spr0479	2.93e-49	essential gene	86.46	98.97	AAK99283
IDKLJMEJ_13	33674	33928	spr1327	4.16e-45	essential gene	94.12	100.00	AAL00131
IDKLJMEJ_21	15506	15769	spr0175	6.41e-43	pathogenitcity related	84.09	100.00	AAK98979
IDKLJMEJ_21	16333	16599	spr0177	1.40e-34	essential gene	84.27	88.12	AAK98981
IDKLJMEJ_64	59249	58749	RopE (M5005_Spy_1611)	1.66e-76	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
IDKLJMEJ_22	57528	59825	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IDKLJMEJ_1	4288	5307	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IDKLJMEJ_1	0.0	1.0	0.0
IDKLJMEJ_2	0.0	1.0	0.0
IDKLJMEJ_3	0.0	1.0	0.0
IDKLJMEJ_4	0.0	1.0	0.0
IDKLJMEJ_5	0.0	1.0	0.0
IDKLJMEJ_6	0.0	1.0	0.0
IDKLJMEJ_7	0.0	1.0	0.0
IDKLJMEJ_8	0.0	1.0	0.0
IDKLJMEJ_9	0.0	1.0	0.0
IDKLJMEJ_10	0.0	1.0	0.0
IDKLJMEJ_11	0.0	1.0	0.0
IDKLJMEJ_12	0.0	1.0	0.0
IDKLJMEJ_13	0.0	1.0	0.0
IDKLJMEJ_14	0.0	1.0	0.0
IDKLJMEJ_15	0.0	1.0	0.0
IDKLJMEJ_16	0.0	1.0	0.0
IDKLJMEJ_17	0.0	1.0	0.0
IDKLJMEJ_18	0.0	1.0	0.0
IDKLJMEJ_19	0.0	1.0	0.0
IDKLJMEJ_20	0.0	1.0	0.0
IDKLJMEJ_21	0.0	1.0	0.0
IDKLJMEJ_22	0.0	1.0	0.0
IDKLJMEJ_23	0.0	1.0	0.0
IDKLJMEJ_24	0.0	1.0	0.0
IDKLJMEJ_25	0.0	1.0	0.0
IDKLJMEJ_26	0.0	1.0	0.0
IDKLJMEJ_27	0.0	1.0	0.0
IDKLJMEJ_28	0.0	1.0	0.0
IDKLJMEJ_29	0.0	1.0	0.0
IDKLJMEJ_30	0.0	1.0	0.0
IDKLJMEJ_31	0.0	1.0	0.0
IDKLJMEJ_32	0.0	1.0	0.0
IDKLJMEJ_33	0.0	1.0	0.0
IDKLJMEJ_34	0.0	1.0	0.0
IDKLJMEJ_35	0.0	1.0	0.0
IDKLJMEJ_36	0.0	1.0	0.0
IDKLJMEJ_37	0.0	1.0	0.0
IDKLJMEJ_38	1.0	0.0	1.0
IDKLJMEJ_39	0.0	1.0	0.0
IDKLJMEJ_40	0.0	1.0	0.0
IDKLJMEJ_41	0.0	1.0	0.0
IDKLJMEJ_42	0.0	1.0	0.0
IDKLJMEJ_43	1.0	0.0	1.0
IDKLJMEJ_44	1.0	0.0	1.0
IDKLJMEJ_45	0.0	1.0	0.0
IDKLJMEJ_46	0.0	1.0	0.0
IDKLJMEJ_47	1.0	0.0	1.0
IDKLJMEJ_48	0.0	1.0	0.0
IDKLJMEJ_50	0.0	1.0	0.0
IDKLJMEJ_53	0.0	1.0	0.0
IDKLJMEJ_58	0.0	1.0	0.0
IDKLJMEJ_60	0.0	1.0	0.0
IDKLJMEJ_64	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found