Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ACGIIBIL_56 | 64489 | 65298 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 95.68 | 100.00 | WP_002948020 |
| ACGIIBIL_3 | 44040 | 45405 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.61 | 99.85 | WP_011681173 |
| ACGIIBIL_3 | 44038 | 45405 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.10 | 100.00 | WP_011226045 |
| ACGIIBIL_56 | 68644 | 69495 | rfbD | 0.0 | Capsule | Immune modulation | 94.48 | 100.00 | WP_014621820 |
| ACGIIBIL_3 | 45462 | 46202 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.20 | 100.00 | WP_011681174 |
| ACGIIBIL_3 | 47645 | 49105 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| ACGIIBIL_3 | 47645 | 49105 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 93.84 | 100.14 | WP_011226049 |
| ACGIIBIL_3 | 45462 | 46202 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.66 | 100.00 | WP_011226046 |
| ACGIIBIL_3 | 47645 | 49105 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| ACGIIBIL_3 | 45467 | 46202 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.21 | 98.13 | WP_011227246 |
| ACGIIBIL_56 | 60448 | 62232 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 92.94 | 100.00 | WP_011226325 |
| ACGIIBIL_3 | 46913 | 47644 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 92.35 | 100.00 | WP_011226048 |
| ACGIIBIL_56 | 63284 | 64489 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 92.21 | 100.00 | WP_011226326 |
| ACGIIBIL_48 | 68654 | 69247 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 91.92 | 100.00 | WP_011226149 |
| ACGIIBIL_56 | 66235 | 67383 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 91.91 | 100.00 | WP_041828290 |
| ACGIIBIL_48 | 68654 | 69247 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 91.75 | 100.00 | WP_011226149 |
| ACGIIBIL_48 | 68654 | 69247 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 91.75 | 100.00 | WP_011226149 |
| ACGIIBIL_3 | 46913 | 47644 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.67 | 100.00 | WP_011681176 |
| ACGIIBIL_56 | 62229 | 63263 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 91.67 | 99.05 | WP_002948022 |
| ACGIIBIL_56 | 65282 | 66238 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 91.64 | 100.00 | WP_011226328 |
| ACGIIBIL_48 | 67785 | 68654 | rfbA | 0.0 | Capsule | Immune modulation | 91.49 | 100.00 | WP_011681286 |
| ACGIIBIL_3 | 46913 | 47644 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.39 | 100.00 | WP_011227248 |
| ACGIIBIL_39 | 82539 | 84197 | fbp54 | 0.0 | FBPs | Adherence | 91.08 | 99.94 | WP_011227179 |
| ACGIIBIL_3 | 46212 | 46904 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.91 | 99.86 | WP_011227247 |
| ACGIIBIL_3 | 46212 | 46904 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.91 | 99.86 | WP_011226047 |
| ACGIIBIL_23 | 258122 | 259132 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.65 | 99.80 | WP_002986042 |
| ACGIIBIL_12 | 189637 | 190940 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.40 | 99.54 | WP_000022832 |
| ACGIIBIL_11 | 18911 | 19750 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.87 | 99.88 | WP_011226733 |
| ACGIIBIL_3 | 46212 | 46904 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.18 | 99.86 | WP_011681175 |
| ACGIIBIL_48 | 69449 | 70495 | rfbB | 0.0 | Capsule | Immune modulation | 84.06 | 99.90 | WP_002263087 |
| ACGIIBIL_33 | 60348 | 61109 | srtA | 0.0 | Sortase A | Exoenzyme | 83.77 | 99.74 | - |
| ACGIIBIL_13 | 31068 | 32351 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.76 | 99.61 | WP_000107753 |
| ACGIIBIL_3 | 32625 | 33208 | SPD_RS01775 | 1.95e-151 | Capsule | Immune modulation | 83.42 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ACGIIBIL_33 | 63796 | 64779 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.95 | 100.00 | AAK75326 |
| ACGIIBIL_33 | 52218 | 51145 | MntE | 0.0 | manganese homeostasis | 95.53 | 99.72 | ABF32167 |
| ACGIIBIL_23 | 129548 | 131854 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| ACGIIBIL_33 | 170081 | 171562 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| ACGIIBIL_33 | 33162 | 30916 | metE | 0.0 | methionine biosynthesis enzymes | 92.66 | 100.00 | AAK74738 |
| ACGIIBIL_12 | 83277 | 84245 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| ACGIIBIL_11 | 34940 | 33969 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| ACGIIBIL_48 | 42403 | 44133 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| ACGIIBIL_56 | 82931 | 80943 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.74 | 100.00 | AE014259_2 |
| ACGIIBIL_9 | 20710 | 19559 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| ACGIIBIL_4 | 45260 | 43782 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| ACGIIBIL_12 | 189637 | 190938 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| ACGIIBIL_4 | 29939 | 28047 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.10 | 98.90 | ABP91129 |
| ACGIIBIL_33 | 27995 | 29704 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| ACGIIBIL_31 | 1860 | 769 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| ACGIIBIL_54 | 19629 | 20639 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| ACGIIBIL_39 | 109520 | 108159 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| ACGIIBIL_12 | 175227 | 176117 | bglB | 3.15e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| ACGIIBIL_39 | 13040 | 12090 | SP_0676 | 2.21e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| ACGIIBIL_33 | 30726 | 29869 | metF | 1.82e-168 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| ACGIIBIL_58 | 61324 | 62028 | covR | 1.85e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| ACGIIBIL_33 | 126685 | 126014 | rr01 | 7.09e-128 | response regulator | 85.27 | 99.56 | CAB54566 |
| ACGIIBIL_33 | 24027 | 23356 | ciaR | 2.21e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| ACGIIBIL_33 | 24027 | 23359 | CiaR | 4.09e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| ACGIIBIL_58 | 61324 | 62028 | walR | 1.16e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| ACGIIBIL_5 | 53810 | 53124 | CovR | 1.35e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| ACGIIBIL_31 | 593 | 198 | SpxA2 | 4.40e-77 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| ACGIIBIL_39 | 120989 | 121390 | SpxA1 | 1.54e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| ACGIIBIL_8 | 6606 | 6193 | GrpE | 9.72e-68 | protein grpE | 81.16 | 80.59 | ABP89267 |
| ACGIIBIL_5 | 30598 | 30311 | spr0479 | 1.64e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| ACGIIBIL_12 | 49175 | 49429 | spr1327 | 1.57e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| ACGIIBIL_54 | 108018 | 108281 | spr0175 | 5.64e-43 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| ACGIIBIL_54 | 108844 | 109110 | spr0177 | 7.86e-42 | essential gene | 83.15 | 88.12 | AAK98981 |
| ACGIIBIL_13 | 30901 | 30410 | RopE (M5005_Spy_1611) | 1.66e-73 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| ACGIIBIL_23 | 129548 | 131848 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| ACGIIBIL_54 | 54749 | 53730 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ACGIIBIL_2 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_3 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_4 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_5 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_6 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_7 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_8 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_9 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_10 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_11 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_12 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_13 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_14 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_15 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_16 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_17 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_18 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_19 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_20 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_21 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_22 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_23 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_25 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_26 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_27 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_28 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_29 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_30 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_31 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_33 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_34 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_36 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_37 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_38 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_39 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_48 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_51 | 1.0 | 0.0 | 1.0 |
| ACGIIBIL_54 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_56 | 0.0 | 1.0 | 0.0 |
| ACGIIBIL_58 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ACGIIBIL_12 | 165478 | 169175 | False | Siphoviridae | VOG6440, VOG0198, VOG0189, VOG4566, VOG8336, VOG8353, VOG2978 |
Phigaro v2.4.0 (Database: Jan. 2024 release)