Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 27 | 33 | 42.64 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EPOOICEK_50 | 60320 | 61189 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| EPOOICEK_97 | 1656 | 2396 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681174 |
| EPOOICEK_97 | 2406 | 3098 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681175 |
| EPOOICEK_97 | 3107 | 3838 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681176 |
| EPOOICEK_97 | 3839 | 5299 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681177 |
| EPOOICEK_50 | 61189 | 61782 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| EPOOICEK_4 | 12308 | 14053 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681434 |
| EPOOICEK_4 | 10606 | 12306 | STER_RS07050 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_041827753 |
| EPOOICEK_4 | 9386 | 10588 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681436 |
| EPOOICEK_4 | 8580 | 9386 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681437 |
| EPOOICEK_4 | 7639 | 8580 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681438 |
| EPOOICEK_4 | 6494 | 7642 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681439 |
| EPOOICEK_4 | 5599 | 6375 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681440 |
| EPOOICEK_4 | 4537 | 5253 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681442 |
| EPOOICEK_4 | 3568 | 4536 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681443 |
| EPOOICEK_4 | 2573 | 3550 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681444 |
| EPOOICEK_4 | 1317 | 2576 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| EPOOICEK_97 | 232 | 1599 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 99.85 | 100.00 | WP_011681173 |
| EPOOICEK_50 | 61189 | 61782 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| EPOOICEK_4 | 10606 | 12306 | STR_RS06890 | 0.0 | Capsule | Immune modulation | 99.71 | 100.00 | WP_041827077 |
| EPOOICEK_50 | 61189 | 61782 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| EPOOICEK_47 | 443 | 2101 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| EPOOICEK_4 | 8580 | 9386 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| EPOOICEK_4 | 7639 | 8580 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | WP_011227430 |
| EPOOICEK_4 | 9386 | 10588 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 99.42 | 100.00 | WP_011227428 |
| EPOOICEK_4 | 6494 | 7642 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.22 | 100.00 | WP_011227431 |
| EPOOICEK_15 | 34376 | 35344 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.86 | 100.00 | WP_082302295 |
| EPOOICEK_38 | 1301 | 2091 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.74 | 100.00 | - |
| EPOOICEK_97 | 1661 | 2396 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.50 | 98.13 | WP_011227246 |
| EPOOICEK_97 | 1656 | 2396 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.25 | 100.00 | WP_011226046 |
| EPOOICEK_97 | 3107 | 3838 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| EPOOICEK_4 | 12308 | 14053 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227426 |
| EPOOICEK_97 | 3107 | 3838 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011226048 |
| EPOOICEK_50 | 25659 | 26420 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| EPOOICEK_97 | 3839 | 5299 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.47 | 100.21 | WP_011226049 |
| EPOOICEK_97 | 3839 | 5299 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_041827043 |
| EPOOICEK_4 | 347 | 1198 | rfbD | 0.0 | Capsule | Immune modulation | 96.83 | 100.00 | WP_014621820 |
| EPOOICEK_97 | 236 | 1599 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.94 | 99.71 | WP_011226045 |
| EPOOICEK_97 | 2406 | 3098 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 89.90 | 100.00 | WP_011226047 |
| EPOOICEK_97 | 2406 | 3098 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 89.61 | 100.00 | WP_011227247 |
| EPOOICEK_12 | 18781 | 20084 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| EPOOICEK_45 | 51 | 1061 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| EPOOICEK_4 | 8580 | 9386 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 82.90 | 100.00 | WP_012000440 |
| EPOOICEK_22 | 33098 | 34381 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| EPOOICEK_50 | 62211 | 63257 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| EPOOICEK_4 | 9386 | 10123 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.96 | 60.29 | WP_012000441 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EPOOICEK_50 | 22972 | 21989 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| EPOOICEK_17 | 27701 | 28774 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| EPOOICEK_23 | 40706 | 43009 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| EPOOICEK_80 | 17885 | 18853 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| EPOOICEK_55 | 403 | 1884 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.51 | 100.00 | ABJ54480 |
| EPOOICEK_42 | 11490 | 9244 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| EPOOICEK_36 | 20654 | 21652 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| EPOOICEK_41 | 4177 | 5148 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| EPOOICEK_39 | 46964 | 45813 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| EPOOICEK_39 | 11865 | 9877 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.14 | 100.00 | AE014259_2 |
| EPOOICEK_50 | 39171 | 40901 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| EPOOICEK_70 | 13824 | 15302 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| EPOOICEK_12 | 20084 | 18783 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| EPOOICEK_70 | 28559 | 30451 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| EPOOICEK_42 | 6319 | 8028 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| EPOOICEK_60 | 22675 | 23685 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| EPOOICEK_20 | 35570 | 36931 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| EPOOICEK_46 | 4864 | 6756 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| EPOOICEK_12 | 34520 | 33630 | bglB | 1.21e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| EPOOICEK_8 | 38571 | 39509 | SP_0676 | 2.03e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| EPOOICEK_42 | 9052 | 8195 | metF | 1.47e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| EPOOICEK_97 | 42122 | 41418 | covR | 1.18e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| EPOOICEK_37 | 3780 | 3109 | rr01 | 3.57e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| EPOOICEK_97 | 42122 | 41418 | walR | 4.97e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| EPOOICEK_42 | 2364 | 1696 | CiaR | 1.96e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| EPOOICEK_4 | 33615 | 32929 | CovR | 2.71e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| EPOOICEK_42 | 2364 | 1693 | ciaR | 1.93e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| EPOOICEK_36 | 21928 | 22302 | SpxA2 | 1.39e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| EPOOICEK_20 | 24207 | 23806 | SpxA1 | 7.78e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| EPOOICEK_21 | 25411 | 25124 | spr0479 | 2.94e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| EPOOICEK_19 | 5684 | 5430 | spr1327 | 4.08e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| EPOOICEK_58 | 1215 | 952 | spr0175 | 4.46e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| EPOOICEK_58 | 388 | 122 | spr0177 | 9.72e-35 | essential gene | 84.27 | 88.12 | AAK98981 |
| EPOOICEK_22 | 32940 | 32440 | RopE (M5005_Spy_1611) | 9.63e-77 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| EPOOICEK_23 | 40706 | 43003 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| EPOOICEK_60 | 58536 | 57517 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EPOOICEK_4 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_8 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_11 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_12 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_15 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_17 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_19 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_20 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_21 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_22 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_23 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_24 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_28 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_29 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_31 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_32 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_34 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_35 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_36 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_37 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_38 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_39 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_40 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_41 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_42 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_43 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_44 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_45 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_46 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_47 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_48 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_49 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_50 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_51 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_52 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_53 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_54 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_55 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_56 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_57 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_58 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_59 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_60 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_61 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_62 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_63 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_64 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_65 | 1.0 | 0.0 | 1.0 |
| EPOOICEK_66 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_67 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_68 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_69 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_70 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_71 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_72 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_73 | 1.0 | 0.0 | 1.0 |
| EPOOICEK_74 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_75 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_76 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_77 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_78 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_80 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_86 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_92 | 0.0 | 1.0 | 0.0 |
| EPOOICEK_97 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)