Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 4 | 6 | 14.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHKJOOJG_3 | 733383 | 733721 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MHKJOOJG_3 | 733066 | 733383 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MHKJOOJG_8 | 567804 | 568658 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| MHKJOOJG_8 | 550730 | 551932 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| MHKJOOJG_8 | 319971 | 321140 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| MHKJOOJG_2 | 275267 | 275860 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| MHKJOOJG_6 | 264209 | 266779 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| MHKJOOJG_4 | 28929 | 30575 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| MHKJOOJG_2 | 28927 | 30573 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| MHKJOOJG_1 | 7928 | 8245 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MHKJOOJG_1 | 7590 | 7928 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MHKJOOJG_8 | 567804 | 568658 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MHKJOOJG_2 | 28927 | 30570 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| MHKJOOJG_4 | 28929 | 30572 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| MHKJOOJG_8 | 567807 | 568658 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| MHKJOOJG_4 | 108638 | 109156 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MHKJOOJG_3 | 307377 | 308558 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MHKJOOJG_8 | 119251 | 119892 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| MHKJOOJG_3 | 306913 | 307362 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| MHKJOOJG_3 | 305752 | 306894 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MHKJOOJG_1 | 319301 | 320077 | CodY | 4.14e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MHKJOOJG_3 | 149250 | 149819 | ClpP | 4.56e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MHKJOOJG_5 | 127366 | 126974 | spxA1 | 2.58e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MHKJOOJG_5 | 127366 | 126974 | spx | 1.54e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MHKJOOJG_4 | 77735 | 77538 | CspA | 6.36e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MHKJOOJG_8 | 131121 | 131315 | CspD | 6.14e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| MHKJOOJG_5 | 372833 | 373027 | CspB | 3.72e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MHKJOOJG_1 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_2 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_3 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_4 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_5 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_6 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_7 | 0.0 | 1.0 | 0.0 |
| MHKJOOJG_8 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MHKJOOJG_5 | 4684 | 29914 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG0749, VOG4685, VOG4773, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| MHKJOOJG_8 | 501868 | 530926 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3892, VOG3891, VOG3890, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG4773, VOG10227, VOG0796, VOG8642, VOG4673, VOG9587, VOG4693, VOG0189, VOG1309 |
Phigaro v2.4.0 (Database: Jan. 2024 release)