Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IOPMGPNG_1 | 2561147 | 2562001 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| IOPMGPNG_1 | 2542331 | 2543533 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| IOPMGPNG_1 | 2340891 | 2342060 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| IOPMGPNG_1 | 1347600 | 1347917 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IOPMGPNG_1 | 1347262 | 1347600 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| IOPMGPNG_1 | 882269 | 884839 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| IOPMGPNG_1 | 586174 | 587817 | vmlR | ARO:3004476 | vmlR | 98.72 | 100.00 | NC_000964.3 |
| IOPMGPNG_1 | 332330 | 332923 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| IOPMGPNG_1 | 281780 | 283213 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| IOPMGPNG_1 | 268969 | 269889 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IOPMGPNG_1 | 4061362 | 4062738 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
| IOPMGPNG_1 | 2561149 | 2562001 | aadK | Streptomycin | 98.12 | 99.77 | M26879 |
| IOPMGPNG_1 | 268969 | 269889 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IOPMGPNG_1 | 4061365 | 4062738 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| IOPMGPNG_1 | 586177 | 587817 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.72 | 100.00 | WP_003234144.1 |
| IOPMGPNG_1 | 268969 | 269886 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| IOPMGPNG_1 | 2561150 | 2562001 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| IOPMGPNG_1 | 4058846 | 4059364 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| IOPMGPNG_1 | 2033235 | 2035832 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IOPMGPNG_1 | 3580182 | 3581363 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| IOPMGPNG_1 | 1982792 | 1984555 | dep/capD | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | NP_389723 |
| IOPMGPNG_1 | 3159414 | 3160199 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| IOPMGPNG_1 | 3155578 | 3156516 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| IOPMGPNG_1 | 3579718 | 3580167 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| IOPMGPNG_1 | 3158192 | 3159388 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| IOPMGPNG_1 | 2141289 | 2141930 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.06 | 100.00 | NP_390062 |
| IOPMGPNG_1 | 3148422 | 3155558 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| IOPMGPNG_1 | 3578557 | 3579699 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| IOPMGPNG_1 | 3156544 | 3158163 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IOPMGPNG_1 | 132400 | 133536 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IOPMGPNG_1 | 18665 | 19534 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IOPMGPNG_1 | 1658074 | 1658850 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IOPMGPNG_1 | 3413821 | 3414390 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IOPMGPNG_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IOPMGPNG_1 | 1291308 | 1317789 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| IOPMGPNG_1 | 2732169 | 2754353 | False | Myoviridae | VOG6116, VOG5859, VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555 |
Phigaro v2.4.0 (Database: Jan. 2024 release)