Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 29 | 33 | 43.93 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCCBJNFP_13 | 9227 | 9820 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| MCCBJNFP_13 | 9820 | 10689 | rfbA | 0.0 | Capsule | Immune modulation | 99.89 | 100.00 | WP_011681286 |
| MCCBJNFP_12 | 38495 | 39304 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.88 | 100.00 | WP_002948020 |
| MCCBJNFP_29 | 9920 | 10612 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 99.86 | 100.00 | WP_011226047 |
| MCCBJNFP_28 | 1352 | 1975 | STU_RS14555 | 0.0 | Capsule | Immune modulation | 99.84 | 100.00 | WP_011226030 |
| MCCBJNFP_13 | 9227 | 9820 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| MCCBJNFP_12 | 36410 | 37558 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | WP_041828290 |
| MCCBJNFP_13 | 9227 | 9820 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| MCCBJNFP_7 | 47184 | 48842 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| MCCBJNFP_47 | 287 | 1430 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.56 | 100.00 | - |
| MCCBJNFP_28 | 123 | 1241 | STU_RS14550 | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | WP_011226029 |
| MCCBJNFP_12 | 41581 | 43365 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.55 | 100.00 | WP_011226325 |
| MCCBJNFP_19 | 315 | 1154 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| MCCBJNFP_29 | 11353 | 12813 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 99.52 | 100.00 | WP_011681177 |
| MCCBJNFP_12 | 37555 | 38511 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.37 | 100.00 | WP_011226328 |
| MCCBJNFP_29 | 9920 | 10612 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011227247 |
| MCCBJNFP_29 | 10621 | 11352 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011226048 |
| MCCBJNFP_12 | 39304 | 40509 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011226326 |
| MCCBJNFP_12 | 40529 | 41584 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | WP_002948022 |
| MCCBJNFP_29 | 10621 | 11352 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011227248 |
| MCCBJNFP_29 | 10621 | 11352 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| MCCBJNFP_8 | 4211 | 5626 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.80 | 100.00 | WP_011681165 |
| MCCBJNFP_12 | 32477 | 33751 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | WP_011226331 |
| MCCBJNFP_12 | 33751 | 34713 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.65 | 95.25 | WP_011226330 |
| MCCBJNFP_21 | 3734 | 4495 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| MCCBJNFP_29 | 11353 | 12813 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.40 | 100.21 | WP_011226049 |
| MCCBJNFP_28 | 2890 | 3339 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 97.33 | 100.00 | WP_011226042 |
| MCCBJNFP_29 | 9170 | 9910 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.17 | 100.00 | WP_011681174 |
| MCCBJNFP_29 | 9170 | 9910 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.03 | 100.00 | WP_011226046 |
| MCCBJNFP_29 | 9175 | 9910 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| MCCBJNFP_29 | 11353 | 12813 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.44 | 100.21 | WP_041827043 |
| MCCBJNFP_12 | 30525 | 31376 | rfbD | 0.0 | Capsule | Immune modulation | 96.24 | 100.00 | WP_014621820 |
| MCCBJNFP_29 | 7746 | 9113 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.81 | 99.93 | WP_011226045 |
| MCCBJNFP_29 | 7748 | 9113 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.44 | 99.71 | WP_011681173 |
| MCCBJNFP_28 | 3348 | 4343 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 92.08 | 99.80 | - |
| MCCBJNFP_29 | 9920 | 10612 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.75 | 100.00 | WP_011681175 |
| MCCBJNFP_55 | 35711 | 37014 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| MCCBJNFP_27 | 14204 | 15209 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 87.40 | 99.31 | WP_002986042 |
| MCCBJNFP_2 | 6176 | 7459 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| MCCBJNFP_13 | 7752 | 8798 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| MCCBJNFP_28 | 2581 | 2890 | STU_RS14615 | 5.54e-111 | Capsule | Immune modulation | 90.32 | 63.58 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCCBJNFP_21 | 1047 | 64 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| MCCBJNFP_66 | 13659 | 12586 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| MCCBJNFP_1 | 15824 | 18127 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| MCCBJNFP_77 | 13162 | 11681 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.71 | 100.00 | ABJ54480 |
| MCCBJNFP_3 | 46507 | 45539 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| MCCBJNFP_25 | 11238 | 8992 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| MCCBJNFP_19 | 19949 | 20947 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| MCCBJNFP_22 | 15769 | 14798 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| MCCBJNFP_44 | 18952 | 17801 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| MCCBJNFP_12 | 17347 | 19335 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.14 | 100.00 | AE014259_2 |
| MCCBJNFP_21 | 17252 | 18982 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| MCCBJNFP_4 | 27286 | 28764 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| MCCBJNFP_55 | 35711 | 37012 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| MCCBJNFP_4 | 42475 | 44367 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| MCCBJNFP_25 | 6067 | 7776 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| MCCBJNFP_18 | 1977 | 967 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| MCCBJNFP_7 | 21943 | 23304 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| MCCBJNFP_5 | 48423 | 46531 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| MCCBJNFP_55 | 21276 | 22166 | bglB | 2.19e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| MCCBJNFP_10 | 4053 | 3115 | SP_0676 | 4.81e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| MCCBJNFP_25 | 8800 | 7943 | metF | 1.38e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| MCCBJNFP_82 | 36905 | 36201 | covR | 7.54e-133 | responder protein | 85.53 | 100.00 | AAK71319 |
| MCCBJNFP_77 | 37827 | 38498 | rr01 | 1.11e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| MCCBJNFP_82 | 36905 | 36201 | walR | 7.09e-123 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| MCCBJNFP_25 | 2113 | 1445 | CiaR | 1.84e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| MCCBJNFP_23 | 47781 | 47095 | CovR | 6.56e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| MCCBJNFP_25 | 2113 | 1442 | ciaR | 1.81e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| MCCBJNFP_19 | 21223 | 21597 | SpxA2 | 1.35e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| MCCBJNFP_7 | 10583 | 10182 | SpxA1 | 9.98e-68 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| MCCBJNFP_23 | 25399 | 25112 | spr0479 | 9.27e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| MCCBJNFP_6 | 30556 | 30810 | spr1327 | 5.49e-45 | essential gene | 94.12 | 100.00 | AAL00131 |
| MCCBJNFP_17 | 17418 | 17155 | spr0175 | 9.26e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| MCCBJNFP_17 | 16591 | 16325 | spr0177 | 2.02e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| MCCBJNFP_2 | 7617 | 8117 | RopE (M5005_Spy_1611) | 1.62e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| MCCBJNFP_1 | 15824 | 18121 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| MCCBJNFP_5 | 33952 | 32933 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCCBJNFP_1 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_2 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_3 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_4 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_5 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_6 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_7 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_8 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_9 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_10 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_11 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_12 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_13 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_14 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_15 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_16 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_17 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_18 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_19 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_20 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_21 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_22 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_23 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_24 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_25 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_26 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_27 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_28 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_29 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_30 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_31 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_32 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_33 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_34 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_35 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_36 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_37 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_38 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_40 | 1.0 | 0.0 | 1.0 |
| MCCBJNFP_41 | 1.0 | 0.0 | 1.0 |
| MCCBJNFP_42 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_43 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_44 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_45 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_46 | 1.0 | 0.0 | 1.0 |
| MCCBJNFP_47 | 1.0 | 0.0 | 1.0 |
| MCCBJNFP_48 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_49 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_50 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_51 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_52 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_55 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_66 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_77 | 0.0 | 1.0 | 0.0 |
| MCCBJNFP_82 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)