Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 33 | 41.40 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KNMGHEDA_23 | 54524 | 55393 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| KNMGHEDA_23 | 53931 | 54524 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| KNMGHEDA_23 | 53931 | 54524 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| KNMGHEDA_55 | 78378 | 79187 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| KNMGHEDA_23 | 53931 | 54524 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| KNMGHEDA_12 | 122829 | 123972 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.56 | 100.00 | - |
| KNMGHEDA_55 | 86308 | 87159 | rfbD | 0.0 | Capsule | Immune modulation | 99.53 | 100.00 | WP_014621820 |
| KNMGHEDA_11 | 13026 | 14684 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| KNMGHEDA_55 | 79171 | 80127 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011226328 |
| KNMGHEDA_55 | 82969 | 83931 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 99.38 | 95.25 | WP_011226330 |
| KNMGHEDA_55 | 74317 | 76101 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226325 |
| KNMGHEDA_44 | 88601 | 90016 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 99.29 | 100.00 | WP_011681165 |
| KNMGHEDA_55 | 77173 | 78378 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011226326 |
| KNMGHEDA_55 | 76098 | 77153 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 99.05 | 100.00 | WP_002948022 |
| KNMGHEDA_44 | 78548 | 79279 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011226048 |
| KNMGHEDA_44 | 80788 | 82155 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 98.83 | 100.00 | WP_011226045 |
| KNMGHEDA_44 | 79990 | 80730 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011681174 |
| KNMGHEDA_44 | 78548 | 79279 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.77 | 100.00 | WP_011227248 |
| KNMGHEDA_44 | 78548 | 79279 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| KNMGHEDA_55 | 80124 | 81272 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_041828290 |
| KNMGHEDA_44 | 79990 | 80725 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.23 | 98.13 | WP_011227246 |
| KNMGHEDA_44 | 79990 | 80730 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011226046 |
| KNMGHEDA_44 | 77087 | 78547 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681177 |
| KNMGHEDA_44 | 79288 | 79980 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| KNMGHEDA_23 | 89293 | 90054 | srtA | 0.0 | Sortase A | Exoenzyme | 97.64 | 100.00 | - |
| KNMGHEDA_12 | 53791 | 54750 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.63 | 99.90 | WP_082302295 |
| KNMGHEDA_44 | 79288 | 79980 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.40 | 100.00 | WP_011227247 |
| KNMGHEDA_44 | 77087 | 78547 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.23 | 100.21 | WP_011226049 |
| KNMGHEDA_44 | 77087 | 78547 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.55 | 100.21 | WP_041827043 |
| KNMGHEDA_44 | 80788 | 82151 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.72 | 99.71 | WP_011681173 |
| KNMGHEDA_44 | 79288 | 79980 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 91.05 | 100.00 | WP_011681175 |
| KNMGHEDA_55 | 83934 | 85205 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 90.18 | 99.69 | WP_011226331 |
| KNMGHEDA_1 | 95899 | 97202 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| KNMGHEDA_22 | 14235 | 15245 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.66 | 99.90 | WP_002986042 |
| KNMGHEDA_76 | 2213 | 3496 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.73 | 99.77 | WP_000107753 |
| KNMGHEDA_23 | 52456 | 53502 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KNMGHEDA_23 | 92741 | 93724 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| KNMGHEDA_1 | 202419 | 203492 | MntE | 0.0 | manganese homeostasis | 94.69 | 99.72 | ABF32167 |
| KNMGHEDA_33 | 66205 | 63902 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| KNMGHEDA_1 | 12257 | 13225 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| KNMGHEDA_77 | 55937 | 57418 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| KNMGHEDA_10 | 31899 | 29653 | metE | 0.0 | methionine biosynthesis enzymes | 91.59 | 100.00 | AAK74738 |
| KNMGHEDA_25 | 1899 | 901 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| KNMGHEDA_14 | 29653 | 28682 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| KNMGHEDA_66 | 47377 | 46226 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| KNMGHEDA_66 | 12009 | 10021 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| KNMGHEDA_23 | 76543 | 74813 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| KNMGHEDA_15 | 1487 | 9 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| KNMGHEDA_1 | 95899 | 97200 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| KNMGHEDA_7 | 13257 | 15149 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.74 | 98.90 | ABP91129 |
| KNMGHEDA_10 | 26728 | 28437 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| KNMGHEDA_18 | 2081 | 1071 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KNMGHEDA_3 | 53031 | 54392 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| KNMGHEDA_5 | 48411 | 46519 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| KNMGHEDA_1 | 81461 | 82351 | bglB | 1.49e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| KNMGHEDA_2 | 44078 | 45016 | SP_0676 | 7.05e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| KNMGHEDA_10 | 29461 | 28604 | metF | 1.09e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| KNMGHEDA_44 | 40256 | 40960 | covR | 2.13e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| KNMGHEDA_77 | 31242 | 30571 | rr01 | 9.74e-125 | response regulator | 83.48 | 99.56 | CAB54566 |
| KNMGHEDA_44 | 40256 | 40960 | walR | 8.98e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KNMGHEDA_4 | 45373 | 46059 | CovR | 2.87e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| KNMGHEDA_10 | 22774 | 22106 | CiaR | 4.11e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| KNMGHEDA_10 | 22774 | 22103 | ciaR | 4.04e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| KNMGHEDA_25 | 625 | 251 | SpxA2 | 9.53e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| KNMGHEDA_3 | 41613 | 41212 | SpxA1 | 1.12e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| KNMGHEDA_9 | 25339 | 25052 | spr0479 | 3.00e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| KNMGHEDA_12 | 95635 | 95889 | spr1327 | 1.31e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| KNMGHEDA_26 | 3972 | 4235 | spr0175 | 4.06e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| KNMGHEDA_26 | 4799 | 5065 | spr0177 | 8.85e-35 | essential gene | 84.27 | 88.12 | AAK98981 |
| KNMGHEDA_76 | 3654 | 4154 | RopE (M5005_Spy_1611) | 1.91e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| KNMGHEDA_33 | 66205 | 63908 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| KNMGHEDA_5 | 33952 | 32933 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KNMGHEDA_1 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_2 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_3 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_4 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_5 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_6 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_7 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_8 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_9 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_10 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_11 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_12 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_13 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_14 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_15 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_16 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_17 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_18 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_19 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_20 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_21 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_22 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_23 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_24 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_25 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_26 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_27 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_28 | 1.0 | 0.0 | 1.0 |
| KNMGHEDA_29 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_30 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_31 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_33 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_34 | 1.0 | 0.0 | 1.0 |
| KNMGHEDA_35 | 1.0 | 0.0 | 1.0 |
| KNMGHEDA_36 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_37 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_38 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_39 | 1.0 | 0.0 | 1.0 |
| KNMGHEDA_40 | 1.0 | 0.0 | 1.0 |
| KNMGHEDA_41 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_42 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_43 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_44 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_55 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_66 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_76 | 0.0 | 1.0 | 0.0 |
| KNMGHEDA_77 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)