Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 7 | 1 | 1 | 7.14 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KCEKGKEI_2 | 137432 | 137920 | nimE | ARO:3007107 | nimE | 80.86 | 95.29 | CP036548.1 |
| KCEKGKEI_301 | 17070 | 19043 | tet(Q) | ARO:3000191 | tet(Q) | 96.35 | 100.00 | Z21523.1 |
| KCEKGKEI_374 | 3678 | 4412 | ErmG | ARO:3000522 | ErmG | 99.18 | 100.00 | L42817.1 |
| KCEKGKEI_331 | 2344 | 3549 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.50 | 100.00 | AF251288.1 |
| KCEKGKEI_359 | 113 | 1078 | CfxA2 | ARO:3003002 | CfxA2 | 99.69 | 100.00 | AF118110.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KCEKGKEI_374 | 3678 | 4412 | erm(G) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.86 | 100.00 | M15332 |
| KCEKGKEI_301 | 17118 | 19043 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 99.84 | 100.00 | L33696 |
| KCEKGKEI_359 | 113 | 1078 | cfxA3 | Ampicillin | 99.79 | 100.00 | AF472622 |
| KCEKGKEI_359 | 113 | 1078 | cfxA4 | Unknown Beta-lactam | 99.79 | 100.00 | AY769933 |
| KCEKGKEI_359 | 113 | 1078 | cfxA5 | Unknown Beta-lactam | 99.79 | 100.00 | AY769934 |
| KCEKGKEI_359 | 113 | 1078 | cfxA | Cefoxitin, Ampicillin | 99.79 | 100.00 | U38243 |
| KCEKGKEI_315 | 42616 | 43833 | mef(A) | Erythromycin, Azithromycin | 94.99 | 100.00 | AF227520 |
| KCEKGKEI_2 | 137432 | 137911 | nimE | Metronidazole | 81.25 | 93.57 | AM042593 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KCEKGKEI_331 | 1810 | 2319 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| KCEKGKEI_359 | 113 | 1075 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 99.69 | 100.00 | WP_004339683.1 |
| KCEKGKEI_374 | 3681 | 4412 | erm(G) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G) | BLASTX | 99.59 | 100.00 | WP_014387027.1 |
| KCEKGKEI_301 | 17118 | 19040 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 99.53 | 100.00 | WP_063856407.1 |
| KCEKGKEI_331 | 2347 | 3549 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.50 | 100.00 | WP_063853729.1 |
| KCEKGKEI_315 | 42616 | 43836 | mef(A) | macrolide efflux MFS transporter Mef(A) | INTERNAL_STOP | 98.53 | 99.75 | WP_012102963.1 |
| KCEKGKEI_2 | 137432 | 137917 | nimE | 5-nitroimidazole reductase NimE | BLASTX | 80.86 | 95.29 | WP_005812773.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KCEKGKEI_92 | 1 | 213 | virulence_entfm_entls | glsB-Entfm | E. faecium variant Efm_DO | 99.53 | 87.65 | CP003583.1 |
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KCEKGKEI_157 | 32324 | 30744 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 80.64 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KCEKGKEI_17 | 26 | 420 | rep14a | 398 / 402 | 82.41 | EFU01917 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KCEKGKEI_1 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_2 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_3 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_4 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_5 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_6 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_7 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_8 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_9 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_10 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_11 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_12 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_13 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_14 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_15 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_24 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_35 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_46 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_57 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_68 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_79 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_90 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_101 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_112 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_113 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_124 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_135 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_146 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_157 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_168 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_179 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_190 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_201 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_212 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_223 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_224 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_235 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_246 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_257 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_268 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_279 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_290 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_301 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_312 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_313 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_314 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_315 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_316 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_317 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_318 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_319 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_320 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_321 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_322 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_323 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_324 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_325 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_326 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_327 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_328 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_329 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_330 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_331 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_332 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_333 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_334 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_335 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_336 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_337 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_338 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_339 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_340 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_341 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_342 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_343 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_344 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_345 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_346 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_347 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_348 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_349 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_350 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_351 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_352 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_353 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_354 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_355 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_356 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_357 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_358 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_359 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_360 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_361 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_362 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_363 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_364 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_365 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_366 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_367 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_368 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_369 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_370 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_372 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_373 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_374 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_375 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_376 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_377 | 1.0 | 0.0 | 1.0 |
| KCEKGKEI_378 | 0.0 | 1.0 | 0.0 |
| KCEKGKEI_379 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KCEKGKEI_223 | 6820 | 17694 | False | Siphoviridae | VOG3992, |
| KCEKGKEI_369 | 24946 | 27591 | False | Myoviridae | VOG10050, |
Phigaro v2.4.0 (Database: Jan. 2024 release)