Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 22 | 33 | 39.66 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHHNPJGN_18 | 10001 | 10870 | rfbA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681286 |
| BHHNPJGN_18 | 9408 | 10001 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| BHHNPJGN_22 | 33841 | 35100 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| BHHNPJGN_18 | 9408 | 10001 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| BHHNPJGN_18 | 9408 | 10001 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| BHHNPJGN_22 | 32868 | 33844 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.59 | 99.90 | WP_011681444 |
| BHHNPJGN_11 | 5807 | 7465 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| BHHNPJGN_1 | 21090 | 21929 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.52 | 100.00 | WP_011226733 |
| BHHNPJGN_22 | 31882 | 32850 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.28 | 100.00 | WP_011681443 |
| BHHNPJGN_19 | 14651 | 15412 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| BHHNPJGN_15 | 17282 | 18013 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011226048 |
| BHHNPJGN_15 | 17282 | 18013 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011227248 |
| BHHNPJGN_15 | 17282 | 18013 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| BHHNPJGN_22 | 31165 | 31880 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.18 | 99.86 | WP_011681442 |
| BHHNPJGN_22 | 30049 | 30819 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.92 | 99.23 | WP_011681440 |
| BHHNPJGN_15 | 7313 | 8728 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681165 |
| BHHNPJGN_15 | 18014 | 19474 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| BHHNPJGN_16 | 21670 | 22468 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 97.62 | 100.88 | - |
| BHHNPJGN_22 | 35219 | 36070 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| BHHNPJGN_15 | 16581 | 17273 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.11 | 100.00 | WP_011226047 |
| BHHNPJGN_15 | 16581 | 17273 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.97 | 100.00 | WP_011227247 |
| BHHNPJGN_15 | 18014 | 19474 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.65 | 100.21 | WP_011226049 |
| BHHNPJGN_15 | 18014 | 19474 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 95.96 | 100.21 | WP_041827043 |
| BHHNPJGN_15 | 14391 | 15756 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 95.46 | 99.85 | WP_011681173 |
| BHHNPJGN_15 | 14389 | 15756 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.08 | 100.00 | WP_011226045 |
| BHHNPJGN_15 | 15813 | 16571 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 93.41 | 100.94 | WP_011681174 |
| BHHNPJGN_15 | 15813 | 16571 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.02 | 100.94 | WP_011226046 |
| BHHNPJGN_15 | 15818 | 16571 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 92.71 | 99.07 | WP_011227246 |
| BHHNPJGN_15 | 16581 | 17273 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.06 | 99.86 | WP_011681175 |
| BHHNPJGN_40 | 95732 | 97035 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| BHHNPJGN_26 | 4709 | 5719 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| BHHNPJGN_18 | 7933 | 8979 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| BHHNPJGN_34 | 5938 | 7221 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHHNPJGN_19 | 18099 | 19082 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| BHHNPJGN_4 | 27708 | 28781 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| BHHNPJGN_24 | 138267 | 140570 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| BHHNPJGN_40 | 12130 | 13098 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| BHHNPJGN_20 | 46213 | 47694 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| BHHNPJGN_6 | 9374 | 11620 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| BHHNPJGN_12 | 12642 | 13634 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| BHHNPJGN_1 | 4608 | 5579 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| BHHNPJGN_24 | 24455 | 23304 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| BHHNPJGN_22 | 49108 | 47120 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| BHHNPJGN_19 | 1901 | 171 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.87 | 100.00 | AAK34199 |
| BHHNPJGN_29 | 94901 | 93423 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| BHHNPJGN_40 | 95732 | 97033 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| BHHNPJGN_29 | 79712 | 77820 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| BHHNPJGN_6 | 14545 | 12836 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| BHHNPJGN_27 | 21347 | 22357 | pdh | 0.0 | pyruvate dehydrogenase | 82.79 | 99.70 | ABP91006 |
| BHHNPJGN_14 | 25729 | 24368 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| BHHNPJGN_28 | 48556 | 46664 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| BHHNPJGN_40 | 81297 | 82187 | bglB | 3.55e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| BHHNPJGN_10 | 10045 | 9110 | SP_0676 | 2.29e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| BHHNPJGN_6 | 11812 | 12669 | metF | 1.67e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| BHHNPJGN_15 | 56294 | 55590 | covR | 1.71e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| BHHNPJGN_20 | 27238 | 26567 | rr01 | 1.05e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| BHHNPJGN_15 | 56294 | 55590 | walR | 7.21e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| BHHNPJGN_6 | 18499 | 19167 | CiaR | 2.22e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| BHHNPJGN_37 | 21755 | 22441 | CovR | 3.84e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| BHHNPJGN_6 | 18499 | 19170 | ciaR | 2.18e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| BHHNPJGN_12 | 13931 | 14305 | SpxA2 | 9.06e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| BHHNPJGN_14 | 37093 | 37494 | SpxA1 | 1.78e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| BHHNPJGN_37 | 44134 | 44421 | spr0479 | 5.33e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| BHHNPJGN_38 | 133438 | 133692 | spr1327 | 1.44e-44 | essential gene | 94.12 | 100.00 | AAL00131 |
| BHHNPJGN_29 | 46083 | 46346 | spr0175 | 4.70e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| BHHNPJGN_29 | 46910 | 47176 | spr0177 | 1.03e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| BHHNPJGN_34 | 7379 | 7879 | RopE (M5005_Spy_1611) | 2.26e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| BHHNPJGN_24 | 138267 | 140564 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| BHHNPJGN_28 | 34082 | 33063 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHHNPJGN_1 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_2 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_3 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_4 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_5 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_6 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_7 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_8 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_9 | 1.0 | 0.0 | 1.0 |
| BHHNPJGN_10 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_11 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_12 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_13 | 1.0 | 0.0 | 1.0 |
| BHHNPJGN_14 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_15 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_16 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_17 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_18 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_19 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_20 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_21 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_22 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_23 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_24 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_25 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_26 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_27 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_28 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_29 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_31 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_32 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_33 | 1.0 | 0.0 | 1.0 |
| BHHNPJGN_34 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_35 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_36 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_37 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_38 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_39 | 0.0 | 1.0 | 0.0 |
| BHHNPJGN_40 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)