safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
1	0	0	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
FCICFAKG_1	1090027	1091946	tet(W)	ARO:3000194	tet(W)	96.71	100.00	AJ222769.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
FCICFAKG_1	1090027	1091946	tet(W)	Doxycycline, Tetracycline, Minocycline	98.96	100.00	FN396364

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
FCICFAKG_1	1090027	1091943	tet(W)	tetracycline resistance ribosomal protection protein Tet(W)	BLASTX	99.53	100.00	WP_000691721.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FCICFAKG_1	0.0	1.0	0.0
FCICFAKG_2	1.0	0.0	1.0
FCICFAKG_3	0.0	1.0	0.0
FCICFAKG_4	0.0	1.0	0.0
FCICFAKG_5	1.0	0.0	1.0
FCICFAKG_6	1.0	0.0	1.0
FCICFAKG_7	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
FCICFAKG_1	62507	94399	False	Siphoviridae	VOG0703, VOG0641, VOG4705, VOG8438, VOG4752, VOG3462, VOG0801, VOG6163, VOG9471, VOG2225, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0866, VOG3691, VOG0198, VOG0686, VOG5317, VOG2246, VOG5280, VOG0195, VOG0275
FCICFAKG_1	94777	98690	False	Unknown	VOG2228, VOG11077, VOG3622, VOG9667
FCICFAKG_1	262907	292451	False	Siphoviridae	VOG0054, VOG1637, VOG9998, VOG4856, VOG9997, VOG4655, VOG10972, VOG3462, VOG0801, VOG4545, VOG0209, VOG4586, VOG0207, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG1345, VOG4544, VOG4581, VOG4841, VOG0866
FCICFAKG_1	319631	331765	False	Siphoviridae	VOG0226, VOG3478, VOG0707, VOG9469, VOG6459, VOG8214, VOG8520, VOG8520, VOG4602, VOG10077, VOG0982, VOG0275
FCICFAKG_1	416241	432371	False	Siphoviridae	VOG0204, VOG0198, VOG4568, VOG4556, VOG4544, VOG4581, VOG4841, VOG0205, VOG4606, VOG0650, VOG9667, VOG0275
FCICFAKG_1	1872105	1901678	False	Siphoviridae	VOG4548, VOG0322, VOG6545, VOG4632, VOG4609, VOG0327, VOG1183, VOG3307, VOG0198, VOG0796, VOG10904, VOG10227, VOG0720, VOG4564, VOG7533, VOG4555, VOG1319, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG4545, VOG4633, VOG4910, VOG5008
FCICFAKG_3	26153	54820	False	Siphoviridae	VOG5088, VOG8294, VOG0641, VOG0648, VOG9888, VOG4619, VOG10608, VOG1335, VOG6163, VOG1334, VOG1333, VOG4634, VOG0704, VOG1900, VOG1330, VOG4713, VOG1887, VOG3434, VOG4555, VOG4564, VOG0720, VOG4544, VOG0796, VOG9331, VOG4972