Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CJLEMKCN_21 | 57908 | 58957 | clbA | ARO:3002814 | clbA | 98.57 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CJLEMKCN_21 | 57922 | 58957 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.42 | 98.67 | KR610408 |
| CJLEMKCN_26 | 215045 | 216431 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 86.23 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CJLEMKCN_21 | 57911 | 58957 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 98.57 | 100.00 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CJLEMKCN_11 | 79959 | 81140 | capB | 0.0 | Capsule | Immune modulation | 82.29 | 99.66 | NP_391471 |
| CJLEMKCN_11 | 79495 | 79944 | capC | 2.16e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CJLEMKCN_30 | 31731 | 32867 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| CJLEMKCN_4 | 4307 | 5176 | pdxS | 1.35e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| CJLEMKCN_1 | 346724 | 345948 | CodY | 1.27e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CJLEMKCN_1 | 820602 | 820210 | spxA1 | 2.32e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| CJLEMKCN_21 | 72498 | 72301 | CspA | 2.35e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| CJLEMKCN_24 | 235219 | 235025 | CspD | 9.74e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| CJLEMKCN_24 | 235219 | 235022 | CspB | 2.02e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| CJLEMKCN_21 | 72498 | 72304 | CspR | 3.08e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| CJLEMKCN_17 | 289407 | 290279 | lipA (SAUSA300_0829) | 1.38e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CJLEMKCN_1 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_2 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_3 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_4 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_5 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_6 | 1.0 | 0.0 | 1.0 |
| CJLEMKCN_7 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_8 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_11 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_12 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_14 | 1.0 | 0.0 | 1.0 |
| CJLEMKCN_17 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_21 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_24 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_26 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_28 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_29 | 0.0 | 1.0 | 0.0 |
| CJLEMKCN_30 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CJLEMKCN_1 | 727045 | 730035 | False | Myoviridae | VOG4885, VOG5447, VOG4861 |
| CJLEMKCN_24 | 191849 | 217160 | False | Siphoviridae | VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG10230 |
Phigaro v2.4.0 (Database: Jan. 2024 release)