Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 0 | 1 | 10.05 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBHJIKKP_1 | 732215 | 733624 | fexB | ARO:3007019 | fexB | 99.36 | 100.00 | JN201336.1 |
| KBHJIKKP_1 | 725208 | 726833 | poxtA | ARO:3004470 | poxtA | 99.07 | 99.82 | MF095097.1 |
| KBHJIKKP_2 | 25768 | 27693 | tet(M) | ARO:3000186 | tet(M) | 98.75 | 100.31 | AB039845.1 |
| KBHJIKKP_2 | 24198 | 25574 | tet(L) | ARO:3000179 | tet(L) | 98.25 | 100.00 | M11036.1 |
| KBHJIKKP_4 | 13249 | 14688 | AAC(6')-Ie-APH(2'')-Ia bifunctional protein | ARO:3002597 | AAC6_Ie_APH2_Ia | 100.00 | 100.00 | GU565967.1 |
| KBHJIKKP_5 | 13066 | 13800 | ErmC | ARO:3000250 | ErmC | 93.44 | 100.00 | M12730.1 |
| KBHJIKKP_4 | 11888 | 12751 | ANT(6)-Ia | ARO:3002626 | ANT(6)-Ia | 100.00 | 100.00 | KF648874.1 |
| KBHJIKKP_5 | 9765 | 10409 | vatE | ARO:3002844 | vatE | 100.00 | 100.00 | AF242872.1 |
| KBHJIKKP_5 | 6315 | 7928 | poxtA | ARO:3004470 | poxtA | 99.44 | 99.08 | MF095097.1 |
| KBHJIKKP_5 | 3221 | 4630 | fexB | ARO:3007019 | fexB | 99.36 | 100.00 | JN201336.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KBHJIKKP_4 | 13249 | 14688 | aac(6')-aph(2'') | Gentamicin, Tobramycin, Netilmicin, Kanamycin, Amikacin, Dibekacin, Isepamicin, Fortimicin, Streptomycin | 100.00 | 100.00 | M13771 |
| KBHJIKKP_4 | 11888 | 12751 | ant(6)-Ia | Streptomycin | 100.00 | 100.00 | KF421157 |
| KBHJIKKP_5 | 9765 | 10409 | vat(E) | Dalfopristin, Pristinamycin IIA, Virginiamycin M | 100.00 | 100.00 | AJ488494 |
| KBHJIKKP_5 | 6315 | 7943 | poxtA | Chloramphenicol, Florfenicol, Linezolid, Doxycycline, Tetracycline | 99.82 | 100.00 | MF095097 |
| KBHJIKKP_1 | 732215 | 733624 | fexB | Chloramphenicol, Florfenicol | 99.79 | 100.00 | JN201336 |
| KBHJIKKP_2 | 24198 | 25573 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.49 | 99.93 | AY081910 |
| KBHJIKKP_2 | 25768 | 27693 | tet(M) | Doxycycline, Tetracycline, Minocycline | 99.48 | 100.00 | X75073 |
| KBHJIKKP_5 | 13066 | 13800 | erm(C) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.32 | 100.00 | Y09003 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KBHJIKKP_4 | 13249 | 14685 | aac(6')-Ie/aph(2'')-Ia | bifunctional aminoglycoside N-acetyltransferase AAC(6')-Ie/aminoglycoside O-phosphotransferase APH(2'')-Ia | EXACTX | 100.00 | 100.00 | WP_001028144.1 |
| KBHJIKKP_4 | 11888 | 12748 | ant(6)-Ia | aminoglycoside nucleotidyltransferase ANT(6)-Ia | EXACTX | 100.00 | 100.00 | WP_002294505.1 |
| KBHJIKKP_5 | 9768 | 10409 | vat(E) | streptogramin A O-acetyltransferase Vat(E) | EXACTX | 100.00 | 100.00 | WP_002328813.1 |
| KBHJIKKP_5 | 6318 | 7943 | poxtA | ABC-F type ribosomal protection protein PoxtA | BLASTX | 99.45 | 100.00 | WP_002415638.1 |
| KBHJIKKP_2 | 25771 | 27693 | tet(M) | tetracycline resistance ribosomal protection protein Tet(M) | BLASTX | 99.38 | 100.00 | WP_000691727.1 |
| KBHJIKKP_1 | 732215 | 733621 | fexB | chloramphenicol/florfenicol efflux MFS transporter FexB | BLASTX | 99.36 | 100.00 | WP_002415211.1 |
| KBHJIKKP_5 | 3224 | 4630 | fexB | chloramphenicol/florfenicol efflux MFS transporter FexB | BLASTX | 99.36 | 100.00 | WP_002415211.1 |
| KBHJIKKP_5 | 13069 | 13800 | erm(C) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(C) | BLASTX | 99.18 | 100.00 | WP_032492416.1 |
| KBHJIKKP_1 | 725208 | 726812 | poxtA | ABC-F type ribosomal protection protein PoxtA | BLASTX | 99.07 | 98.71 | WP_002415638.1 |
| KBHJIKKP_2 | 24201 | 25574 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_002294500.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KBHJIKKP_1 | 1048485 | 1047778 | WalR | 5.52e-115 | transcriptional regulatory protein | 85.17 | 100.43 | EPH95667 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KBHJIKKP_5 | 1 | 1494 | rep2 | 1494 / 1494 | 100.00 | X92945 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KBHJIKKP_1 | 0.0 | 1.0 | 0.0 |
| KBHJIKKP_2 | 1.0 | 0.0 | 1.0 |
| KBHJIKKP_4 | 1.0 | 0.0 | 1.0 |
| KBHJIKKP_5 | 1.0 | 0.0 | 1.0 |
| KBHJIKKP_6 | 1.0 | 0.0 | 1.0 |
| KBHJIKKP_7 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KBHJIKKP_1 | 454739 | 478200 | False | Siphoviridae | VOG10868, VOG4683, VOG3462, VOG0801, VOG6163, VOG0817, VOG0725, VOG0799, VOG0704, VOG4713, VOG1329, VOG4555, VOG4564, VOG0720, VOG4544, VOG1517, VOG4618, VOG4618, VOG0376, VOG2545, VOG0198 |
| KBHJIKKP_1 | 1695686 | 1702385 | False | Siphoviridae | VOG4632, VOG7006, VOG8353, VOG0831, VOG4552, VOG0437, VOG8520, VOG8520, VOG4602, VOG0275 |
| KBHJIKKP_4 | 96652 | 98491 | False | Myoviridae | VOG5458, VOG8288, VOG0267, VOG0267, VOG0267, VOG0267 |
| KBHJIKKP_4 | 232891 | 255356 | False | Siphoviridae | VOG8733, VOG4705, VOG3390, VOG10868, VOG4683, VOG4619, VOG0801, VOG5793, VOG0701, VOG9763, VOG0698, VOG0697, VOG0696, VOG0695, VOG4555, VOG0692, VOG0691, VOG4544, VOG3699 |
| KBHJIKKP_4 | 262958 | 265822 | False | Siphoviridae | VOG0514, VOG6545, VOG0322, VOG0535 |
Phigaro v2.4.0 (Database: Jan. 2024 release)