Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGKBOILC_10 | 1395556 | 1396410 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| MGKBOILC_10 | 1377183 | 1378385 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| MGKBOILC_10 | 1157953 | 1159122 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MGKBOILC_7 | 412604 | 414247 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| MGKBOILC_8 | 265980 | 268550 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| MGKBOILC_7 | 163747 | 164340 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| MGKBOILC_7 | 113185 | 114624 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| MGKBOILC_7 | 100370 | 101290 | mphK | ARO:3004541 | mphK | 98.02 | 100.00 | CP010314.1 |
| MGKBOILC_10 | 41666 | 41983 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MGKBOILC_10 | 41328 | 41666 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MGKBOILC_10 | 1395558 | 1396410 | aadK | Streptomycin | 98.94 | 99.77 | M26879 |
| MGKBOILC_12 | 322430 | 323806 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.91 | 100.00 | D12567 |
| MGKBOILC_7 | 100370 | 101290 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGKBOILC_10 | 1395559 | 1396410 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| MGKBOILC_12 | 322433 | 323806 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| MGKBOILC_7 | 412607 | 414247 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| MGKBOILC_7 | 100370 | 101278 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.02 | 99.02 | WP_003246254.1 |
| MGKBOILC_12 | 319959 | 320477 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MGKBOILC_10 | 850130 | 852727 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MGKBOILC_11 | 109176 | 110114 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| MGKBOILC_11 | 102020 | 109156 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.78 | 100.00 | NP_391076 |
| MGKBOILC_11 | 527869 | 529050 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| MGKBOILC_11 | 110142 | 111761 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391078 |
| MGKBOILC_11 | 527405 | 527854 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| MGKBOILC_11 | 113012 | 113797 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| MGKBOILC_10 | 800826 | 802589 | dep/capD | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | NP_389723 |
| MGKBOILC_11 | 111790 | 112986 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| MGKBOILC_10 | 957984 | 958625 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| MGKBOILC_11 | 526244 | 527386 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MGKBOILC_6 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MGKBOILC_1 | 4308 | 5177 | pdxS | 1.95e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| MGKBOILC_10 | 354960 | 355736 | CodY | 1.49e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MGKBOILC_11 | 368666 | 369235 | ClpP | 4.14e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MGKBOILC_9 | 276425 | 276817 | spxA1 | 2.48e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MGKBOILC_9 | 276425 | 276817 | spx | 1.48e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| MGKBOILC_5 | 20590 | 20832 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| MGKBOILC_7 | 364678 | 364875 | CspA | 1.91e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MGKBOILC_9 | 33198 | 33004 | CspB | 3.58e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MGKBOILC_1 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_2 | 1.0 | 0.0 | 1.0 |
| MGKBOILC_3 | 1.0 | 0.0 | 1.0 |
| MGKBOILC_5 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_6 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_7 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_8 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_9 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_10 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_11 | 0.0 | 1.0 | 0.0 |
| MGKBOILC_12 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MGKBOILC_3 | 9291 | 33027 | False | Siphoviridae | VOG0221, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024 |
| MGKBOILC_9 | 374077 | 383999 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685 |
| MGKBOILC_10 | 4421 | 11518 | False | Myoviridae | VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| MGKBOILC_10 | 632385 | 643207 | False | Siphoviridae | VOG6211, VOG0322, VOG7476, VOG0025, VOG4551, VOG0918 |
| MGKBOILC_10 | 1330629 | 1360655 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)