Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 10 | 5 | 14.35 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGOJJELI_2 | 1852816 | 1853736 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| MGOJJELI_2 | 1839490 | 1840923 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
| MGOJJELI_2 | 1788302 | 1788895 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| MGOJJELI_2 | 1521387 | 1523033 | vmlR | ARO:3004476 | vmlR | 98.18 | 100.18 | NC_000964.3 |
| MGOJJELI_2 | 1237125 | 1239695 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| MGOJJELI_2 | 739877 | 740215 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| MGOJJELI_2 | 739560 | 739877 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| MGOJJELI_1 | 318677 | 319846 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MGOJJELI_2 | 1852816 | 1853736 | mph(K) | Spiramycin, Telithromycin | 97.50 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MGOJJELI_2 | 1521387 | 1523030 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.18 | 100.00 | WP_003234144.1 |
| MGOJJELI_2 | 1852819 | 1853736 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| MGOJJELI_2 | 2147155 | 2147673 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MGOJJELI_2 | 2620832 | 2622013 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| MGOJJELI_2 | 2622495 | 2623637 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| MGOJJELI_2 | 3041510 | 3042295 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| MGOJJELI_2 | 2622028 | 2622476 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 99.78 | NP_391470 |
| MGOJJELI_2 | 165198 | 166961 | dep/capD | 0.0 | Capsule | Immune modulation | 98.81 | 100.00 | NP_389723 |
| MGOJJELI_1 | 1055762 | 1056554 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 84.45 | NP_391077 |
| MGOJJELI_2 | 3042321 | 3043517 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| MGOJJELI_1 | 1048606 | 1055742 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.98 | 100.00 | NP_391076 |
| MGOJJELI_1 | 115131 | 115772 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.82 | 100.00 | NP_390062 |
| MGOJJELI_2 | 3043535 | 3045153 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.28 | 99.94 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MGOJJELI_2 | 1989267 | 1988131 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| MGOJJELI_2 | 2103433 | 2102564 | pdxS | 2.27e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MGOJJELI_2 | 419546 | 418770 | CodY | 2.48e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MGOJJELI_2 | 2767317 | 2766748 | ClpP | 1.84e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| MGOJJELI_2 | 937943 | 937551 | spxA1 | 1.95e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| MGOJJELI_2 | 937943 | 937551 | spx | 1.16e-67 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MGOJJELI_1 | 0.0 | 1.0 | 0.0 |
| MGOJJELI_2 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MGOJJELI_1 | 54394 | 76948 | False | Siphoviridae | VOG7718, VOG3390, VOG4705, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4660, VOG8440 |
| MGOJJELI_2 | 273201 | 304926 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4581, VOG4841, VOG0198, VOG1884, VOG0025, VOG0025, VOG9600, VOG11060, VOG4552, VOG0737, VOG0181 |
| MGOJJELI_2 | 769711 | 796326 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG9315, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667, VOG4602 |
| MGOJJELI_2 | 869219 | 888346 | False | Siphoviridae | VOG5535, VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG6548, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0691, VOG10227, VOG0796, VOG0052 |
| MGOJJELI_2 | 891570 | 902716 | False | Siphoviridae | VOG0536, VOG0044, VOG0189, VOG4566, VOG1309, VOG4600, VOG0125, VOG1878, VOG4552, VOG8241, VOG4603, VOG6495, VOG4662, VOG4602, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)