Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ACDAMKMM_1 | 2737460 | 2738314 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| ACDAMKMM_1 | 2718788 | 2719990 | blt | ARO:3003006 | blt | 98.75 | 100.00 | L32599.1 |
| ACDAMKMM_1 | 2516387 | 2517556 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| ACDAMKMM_1 | 1357500 | 1357817 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ACDAMKMM_1 | 1357162 | 1357500 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ACDAMKMM_1 | 894699 | 897269 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| ACDAMKMM_1 | 592786 | 594429 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
| ACDAMKMM_1 | 340861 | 341454 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| ACDAMKMM_1 | 290308 | 291741 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| ACDAMKMM_1 | 277494 | 278414 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ACDAMKMM_1 | 277494 | 278414 | mph(K) | Spiramycin, Telithromycin | 99.13 | 100.00 | NC_000964 |
| ACDAMKMM_1 | 2737462 | 2738314 | aadK | Streptomycin | 98.83 | 99.77 | M26879 |
| ACDAMKMM_1 | 4200706 | 4202082 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.40 | 100.00 | D12567 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ACDAMKMM_1 | 277494 | 278411 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| ACDAMKMM_1 | 592789 | 594429 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| ACDAMKMM_1 | 2737463 | 2738314 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| ACDAMKMM_1 | 4200709 | 4202082 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.25 | 100.00 | WP_063856081.1 |
| ACDAMKMM_1 | 4198183 | 4198701 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| ACDAMKMM_1 | 2084117 | 2086714 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ACDAMKMM_1 | 3293244 | 3294029 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| ACDAMKMM_1 | 2317181 | 2317822 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| ACDAMKMM_1 | 3705977 | 3707158 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| ACDAMKMM_1 | 3704352 | 3705494 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| ACDAMKMM_1 | 3705513 | 3705962 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| ACDAMKMM_1 | 3289408 | 3290346 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| ACDAMKMM_1 | 3292022 | 3293218 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| ACDAMKMM_1 | 2033977 | 2035740 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| ACDAMKMM_1 | 3282252 | 3289388 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391076 |
| ACDAMKMM_1 | 3290374 | 3291993 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.03 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ACDAMKMM_1 | 132474 | 133610 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ACDAMKMM_1 | 18665 | 19534 | pdxS | 3.14e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ACDAMKMM_1 | 1696488 | 1697264 | CodY | 3.43e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ACDAMKMM_1 | 3544628 | 3545197 | ClpP | 2.55e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ACDAMKMM_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ACDAMKMM_1 | 1299867 | 1327362 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| ACDAMKMM_1 | 2194080 | 2203905 | False | Siphoviridae | VOG1198, VOG0918, VOG4551, VOG0025, VOG7476 |
| ACDAMKMM_1 | 2263646 | 2282232 | False | Unknown | VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900 |
Phigaro v2.4.0 (Database: Jan. 2024 release)