Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EENHFNNH_1 | 3981880 | 3982473 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| EENHFNNH_1 | 3931318 | 3932751 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| EENHFNNH_1 | 3918500 | 3919420 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| EENHFNNH_1 | 2134314 | 2135168 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| EENHFNNH_1 | 2115625 | 2116827 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| EENHFNNH_1 | 1894215 | 1895384 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EENHFNNH_1 | 897108 | 897425 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EENHFNNH_1 | 896770 | 897108 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EENHFNNH_1 | 435881 | 438451 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| EENHFNNH_1 | 138910 | 140553 | vmlR | ARO:3004476 | vmlR | 98.90 | 100.00 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EENHFNNH_1 | 3614276 | 3615652 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.49 | 100.00 | D12567 |
| EENHFNNH_1 | 2134316 | 2135168 | aadK | Streptomycin | 99.30 | 99.77 | M26879 |
| EENHFNNH_1 | 3918500 | 3919420 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EENHFNNH_1 | 3614279 | 3615652 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| EENHFNNH_1 | 3918500 | 3919417 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| EENHFNNH_1 | 138913 | 140553 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.90 | 100.00 | WP_003234144.1 |
| EENHFNNH_1 | 2134317 | 2135168 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| EENHFNNH_1 | 3611755 | 3612273 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| EENHFNNH_1 | 1586820 | 1589417 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EENHFNNH_1 | 2692011 | 2692796 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| EENHFNNH_1 | 2688175 | 2689113 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| EENHFNNH_1 | 2681020 | 2688155 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.59 | 99.97 | NP_391076 |
| EENHFNNH_1 | 3106250 | 3107431 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| EENHFNNH_1 | 3104625 | 3105767 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| EENHFNNH_1 | 1694819 | 1695460 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| EENHFNNH_1 | 3105786 | 3106235 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| EENHFNNH_1 | 2690789 | 2691985 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391079 |
| EENHFNNH_1 | 1536825 | 1538588 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| EENHFNNH_1 | 2689141 | 2690760 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EENHFNNH_1 | 3773210 | 3774346 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EENHFNNH_1 | 3659483 | 3660352 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EENHFNNH_1 | 1207283 | 1208059 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EENHFNNH_1 | 2944077 | 2944646 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EENHFNNH_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EENHFNNH_1 | 840838 | 867291 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| EENHFNNH_1 | 2065926 | 2095962 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)