Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFDCLBDA_1 | 3038803 | 3039657 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| KFDCLBDA_1 | 3015842 | 3017044 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| KFDCLBDA_1 | 2788920 | 2790089 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KFDCLBDA_1 | 1655921 | 1656238 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KFDCLBDA_1 | 1655583 | 1655921 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| KFDCLBDA_1 | 1156228 | 1158798 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| KFDCLBDA_1 | 607523 | 608116 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| KFDCLBDA_1 | 559605 | 561038 | lmrB | ARO:3002813 | lmrB | 98.95 | 100.00 | JYFL01000006.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KFDCLBDA_1 | 3038805 | 3039657 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| KFDCLBDA_1 | 533410 | 534329 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KFDCLBDA_1 | 3038806 | 3039657 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| KFDCLBDA_1 | 853767 | 855407 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 97.81 | 100.00 | WP_003234144.1 |
| KFDCLBDA_1 | 233223 | 233615 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 93.13 | 75.72 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KFDCLBDA_1 | 3596170 | 3597108 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| KFDCLBDA_1 | 4008771 | 4009952 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| KFDCLBDA_1 | 4007146 | 4008288 | capA | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391469 |
| KFDCLBDA_1 | 4008307 | 4008756 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| KFDCLBDA_1 | 2586131 | 2586772 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| KFDCLBDA_1 | 3599995 | 3600780 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| KFDCLBDA_1 | 2325008 | 2326771 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| KFDCLBDA_1 | 3589014 | 3596150 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.78 | 100.00 | NP_391076 |
| KFDCLBDA_1 | 3598773 | 3599969 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| KFDCLBDA_1 | 3597136 | 3598755 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.78 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KFDCLBDA_1 | 392872 | 394008 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KFDCLBDA_1 | 277382 | 278251 | pdxS | 3.17e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KFDCLBDA_1 | 1967586 | 1968362 | CodY | 3.46e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KFDCLBDA_1 | 3857208 | 3857777 | ClpP | 2.57e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KFDCLBDA_1 | 0.0 | 1.0 | 0.0 |
| KFDCLBDA_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KFDCLBDA_1 | 1599672 | 1625776 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| KFDCLBDA_1 | 2097924 | 2120505 | True | Siphoviridae | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG8610, VOG5008, VOG4705, VOG3390, VOG7718, VOG5534, VOG6940, VOG0944, VOG5532, VOG9713 |
| KFDCLBDA_1 | 2466890 | 2471771 | False | Siphoviridae | VOG0918, VOG4551, VOG0025 |
| KFDCLBDA_1 | 2530406 | 2550614 | False | Siphoviridae | VOG7718, VOG3390, VOG7895, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG5070, VOG10230 |
| KFDCLBDA_1 | 2963517 | 2988190 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG7060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)