safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	16	1	16.03

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
BFCFNPKO_121	18261	18737	aaiA	0.0	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_000072549
BFCFNPKO_121	15832	16254	aaiD	0.0	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_000555547
BFCFNPKO_121	10641	11108	aaiH	0.0	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_000005080
BFCFNPKO_121	9252	10328	aaiJ	0.0	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_001033155
BFCFNPKO_121	11119	13101	vgrG	0.0	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_000198270
BFCFNPKO_121	14037	15839	aaiE	0.0	AAI/SCI-II T6SS	Effector delivery system	99.94	100.00	WP_001390300
BFCFNPKO_121	2923	6327	icmF/aaiO	0.0	AAI/SCI-II T6SS	Effector delivery system	99.94	100.00	WP_000914956
BFCFNPKO_121	16780	18258	aaiB	0.0	AAI/SCI-II T6SS	Effector delivery system	99.93	100.00	WP_000215053
BFCFNPKO_154	755	1909	EC042_RS24460	0.0	AAI/SCI-II T6SS	Effector delivery system	99.91	100.00	WP_000359994
BFCFNPKO_121	13114	14046	aaiF	0.0	AAI/SCI-II T6SS	Effector delivery system	99.89	100.00	WP_001173975
BFCFNPKO_121	370	2919	clpV/aaiP	0.0	AAI/SCI-II T6SS	Effector delivery system	99.88	100.00	WP_000029846
BFCFNPKO_121	7262	8674	aaiL	0.0	AAI/SCI-II T6SS	Effector delivery system	99.86	100.00	WP_001154665
BFCFNPKO_121	6333	6923	aaiN	0.0	AAI/SCI-II T6SS	Effector delivery system	99.83	100.00	WP_001028113
BFCFNPKO_121	8693	9244	aaiK	0.0	AAI/SCI-II T6SS	Effector delivery system	99.82	100.00	WP_000152746
BFCFNPKO_121	16264	16770	aaiC/hcp	0.0	AAI/SCI-II T6SS	Effector delivery system	99.80	100.00	WP_000233190
BFCFNPKO_121	10332	10631	aaiI	7.41e-156	AAI/SCI-II T6SS	Effector delivery system	100.00	100.00	WP_001390299

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
BFCFNPKO_121	16264	16770	virulence_ecoli	aaiC	Type VI secretion protein	100.0	100.00	FN554766

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
BFCFNPKO_121	16770	16267	Hcp2	1.71e-109	type VI secretion system protein	100.00	100.00	EFG2884257

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
BFCFNPKO_1	0.0	1.0	0.0
BFCFNPKO_2	0.0	1.0	0.0
BFCFNPKO_3	0.0	1.0	0.0
BFCFNPKO_4	0.0	1.0	0.0
BFCFNPKO_5	1.0	0.0	1.0
BFCFNPKO_6	0.0	1.0	0.0
BFCFNPKO_7	0.0	1.0	0.0
BFCFNPKO_8	0.0	1.0	0.0
BFCFNPKO_9	0.0	1.0	0.0
BFCFNPKO_10	0.0	1.0	0.0
BFCFNPKO_11	1.0	0.0	1.0
BFCFNPKO_12	1.0	0.0	1.0
BFCFNPKO_13	1.0	0.0	1.0
BFCFNPKO_14	0.0	1.0	0.0
BFCFNPKO_15	0.0	1.0	0.0
BFCFNPKO_16	0.0	1.0	0.0
BFCFNPKO_17	1.0	0.0	1.0
BFCFNPKO_18	0.0	1.0	0.0
BFCFNPKO_19	0.0	1.0	0.0
BFCFNPKO_20	0.0	1.0	0.0
BFCFNPKO_21	1.0	0.0	1.0
BFCFNPKO_22	1.0	0.0	1.0
BFCFNPKO_23	1.0	0.0	1.0
BFCFNPKO_24	0.0	1.0	0.0
BFCFNPKO_25	0.0	1.0	0.0
BFCFNPKO_26	0.0	1.0	0.0
BFCFNPKO_27	0.0	1.0	0.0
BFCFNPKO_28	1.0	0.0	1.0
BFCFNPKO_29	1.0	0.0	1.0
BFCFNPKO_31	1.0	0.0	1.0
BFCFNPKO_32	0.0	1.0	0.0
BFCFNPKO_33	0.0	1.0	0.0
BFCFNPKO_34	0.0	1.0	0.0
BFCFNPKO_35	1.0	0.0	1.0
BFCFNPKO_36	0.0	1.0	0.0
BFCFNPKO_37	0.0	1.0	0.0
BFCFNPKO_38	1.0	0.0	1.0
BFCFNPKO_39	1.0	0.0	1.0
BFCFNPKO_40	1.0	0.0	1.0
BFCFNPKO_41	0.0	1.0	0.0
BFCFNPKO_42	0.0	1.0	0.0
BFCFNPKO_43	0.0	1.0	0.0
BFCFNPKO_44	0.0	1.0	0.0
BFCFNPKO_45	0.0	1.0	0.0
BFCFNPKO_46	0.0	1.0	0.0
BFCFNPKO_47	0.0	1.0	0.0
BFCFNPKO_48	0.0	1.0	0.0
BFCFNPKO_49	1.0	0.0	1.0
BFCFNPKO_50	0.0	1.0	0.0
BFCFNPKO_51	0.0	1.0	0.0
BFCFNPKO_52	0.0	1.0	0.0
BFCFNPKO_53	0.0	1.0	0.0
BFCFNPKO_54	0.0	1.0	0.0
BFCFNPKO_55	0.0	1.0	0.0
BFCFNPKO_56	1.0	0.0	1.0
BFCFNPKO_57	1.0	0.0	1.0
BFCFNPKO_58	0.0	1.0	0.0
BFCFNPKO_59	0.0	1.0	0.0
BFCFNPKO_60	0.0	1.0	0.0
BFCFNPKO_61	0.0	1.0	0.0
BFCFNPKO_62	0.0	1.0	0.0
BFCFNPKO_63	0.0	1.0	0.0
BFCFNPKO_64	0.0	1.0	0.0
BFCFNPKO_65	0.0	1.0	0.0
BFCFNPKO_66	0.0	1.0	0.0
BFCFNPKO_67	0.0	1.0	0.0
BFCFNPKO_68	0.0	1.0	0.0
BFCFNPKO_69	0.0	1.0	0.0
BFCFNPKO_70	0.0	1.0	0.0
BFCFNPKO_71	0.0	1.0	0.0
BFCFNPKO_72	0.0	1.0	0.0
BFCFNPKO_73	0.0	1.0	0.0
BFCFNPKO_74	0.0	1.0	0.0
BFCFNPKO_75	0.0	1.0	0.0
BFCFNPKO_76	0.0	1.0	0.0
BFCFNPKO_77	0.0	1.0	0.0
BFCFNPKO_78	0.0	1.0	0.0
BFCFNPKO_79	0.0	1.0	0.0
BFCFNPKO_80	0.0	1.0	0.0
BFCFNPKO_81	0.0	1.0	0.0
BFCFNPKO_82	1.0	0.0	1.0
BFCFNPKO_83	0.0	1.0	0.0
BFCFNPKO_84	0.0	1.0	0.0
BFCFNPKO_85	0.0	1.0	0.0
BFCFNPKO_86	0.0	1.0	0.0
BFCFNPKO_87	0.0	1.0	0.0
BFCFNPKO_88	0.0	1.0	0.0
BFCFNPKO_89	0.0	1.0	0.0
BFCFNPKO_90	0.0	1.0	0.0
BFCFNPKO_91	0.0	1.0	0.0
BFCFNPKO_92	0.0	1.0	0.0
BFCFNPKO_93	1.0	0.0	1.0
BFCFNPKO_94	0.0	1.0	0.0
BFCFNPKO_95	0.0	1.0	0.0
BFCFNPKO_96	1.0	0.0	1.0
BFCFNPKO_97	1.0	0.0	1.0
BFCFNPKO_98	0.0	1.0	0.0
BFCFNPKO_99	0.0	1.0	0.0
BFCFNPKO_100	1.0	0.0	1.0
BFCFNPKO_101	0.0	1.0	0.0
BFCFNPKO_102	0.0	1.0	0.0
BFCFNPKO_103	0.0	1.0	0.0
BFCFNPKO_104	0.0	1.0	0.0
BFCFNPKO_105	0.0	1.0	0.0
BFCFNPKO_106	0.0	1.0	0.0
BFCFNPKO_107	0.0	1.0	0.0
BFCFNPKO_108	0.0	1.0	0.0
BFCFNPKO_109	0.0	1.0	0.0
BFCFNPKO_110	1.0	0.0	1.0
BFCFNPKO_111	0.0	1.0	0.0
BFCFNPKO_112	0.0	1.0	0.0
BFCFNPKO_113	0.0	1.0	0.0
BFCFNPKO_114	1.0	0.0	1.0
BFCFNPKO_115	0.0	1.0	0.0
BFCFNPKO_116	0.0	1.0	0.0
BFCFNPKO_117	0.0	1.0	0.0
BFCFNPKO_118	0.0	1.0	0.0
BFCFNPKO_119	0.0	1.0	0.0
BFCFNPKO_120	0.0	1.0	0.0
BFCFNPKO_121	0.0	1.0	0.0
BFCFNPKO_122	0.0	1.0	0.0
BFCFNPKO_123	1.0	0.0	1.0
BFCFNPKO_124	0.0	1.0	0.0
BFCFNPKO_125	0.0	1.0	0.0
BFCFNPKO_126	1.0	0.0	1.0
BFCFNPKO_127	0.0	1.0	0.0
BFCFNPKO_128	0.0	1.0	0.0
BFCFNPKO_129	0.0	1.0	0.0
BFCFNPKO_130	0.0	1.0	0.0
BFCFNPKO_131	1.0	0.0	1.0
BFCFNPKO_132	0.0	1.0	0.0
BFCFNPKO_133	0.0	1.0	0.0
BFCFNPKO_134	1.0	0.0	1.0
BFCFNPKO_135	0.0	1.0	0.0
BFCFNPKO_136	1.0	0.0	1.0
BFCFNPKO_137	0.0	1.0	0.0
BFCFNPKO_138	1.0	0.0	1.0
BFCFNPKO_139	1.0	0.0	1.0
BFCFNPKO_140	0.0	1.0	0.0
BFCFNPKO_141	0.0	1.0	0.0
BFCFNPKO_142	1.0	0.0	1.0
BFCFNPKO_143	1.0	0.0	1.0
BFCFNPKO_144	0.0	1.0	0.0
BFCFNPKO_145	0.0	1.0	0.0
BFCFNPKO_146	0.0	1.0	0.0
BFCFNPKO_147	0.0	1.0	0.0
BFCFNPKO_148	0.0	1.0	0.0
BFCFNPKO_149	1.0	0.0	1.0
BFCFNPKO_150	0.0	1.0	0.0
BFCFNPKO_151	0.0	1.0	0.0
BFCFNPKO_152	0.0	1.0	0.0
BFCFNPKO_153	0.0	1.0	0.0
BFCFNPKO_154	1.0	0.0	1.0
BFCFNPKO_155	0.0	1.0	0.0
BFCFNPKO_156	1.0	0.0	1.0
BFCFNPKO_157	0.0	1.0	0.0
BFCFNPKO_158	0.0	1.0	0.0
BFCFNPKO_159	0.0	1.0	0.0
BFCFNPKO_160	0.0	1.0	0.0
BFCFNPKO_162	0.0	1.0	0.0
BFCFNPKO_163	1.0	0.0	1.0
BFCFNPKO_164	0.0	1.0	0.0
BFCFNPKO_165	0.0	1.0	0.0
BFCFNPKO_166	0.0	1.0	0.0
BFCFNPKO_167	1.0	0.0	1.0
BFCFNPKO_168	0.0	1.0	0.0
BFCFNPKO_169	0.0	1.0	0.0
BFCFNPKO_170	0.0	1.0	0.0
BFCFNPKO_171	0.0	1.0	0.0
BFCFNPKO_172	1.0	0.0	1.0
BFCFNPKO_173	0.0	1.0	0.0
BFCFNPKO_174	0.0	1.0	0.0
BFCFNPKO_175	0.0	1.0	0.0
BFCFNPKO_176	1.0	0.0	1.0
BFCFNPKO_177	0.0	1.0	0.0
BFCFNPKO_178	0.0	1.0	0.0
BFCFNPKO_179	1.0	0.0	1.0
BFCFNPKO_180	0.0	1.0	0.0
BFCFNPKO_181	0.0	1.0	0.0
BFCFNPKO_182	0.0	1.0	0.0
BFCFNPKO_183	0.0	1.0	0.0
BFCFNPKO_184	0.0	1.0	0.0
BFCFNPKO_185	1.0	0.0	1.0
BFCFNPKO_186	0.0	1.0	0.0
BFCFNPKO_187	0.0	1.0	0.0
BFCFNPKO_188	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found