Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBEMHNKK_9 | 50514 | 51563 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MBEMHNKK_9 | 50528 | 51563 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 89.00 | 98.67 | KR610408 |
| MBEMHNKK_1 | 374849 | 375789 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 85.35 | 68.34 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MBEMHNKK_9 | 50517 | 51563 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| MBEMHNKK_2 | 173172 | 175766 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.11 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MBEMHNKK_3 | 144296 | 145477 | capB | 0.0 | Capsule | Immune modulation | 82.12 | 99.66 | NP_391471 |
| MBEMHNKK_3 | 143832 | 144281 | capC | 1.89e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MBEMHNKK_10 | 31636 | 32772 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| MBEMHNKK_14 | 4267 | 5136 | pdxS | 1.34e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| MBEMHNKK_2 | 375398 | 374622 | CodY | 8.61e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MBEMHNKK_5 | 83408 | 83016 | spxA1 | 7.29e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MBEMHNKK_9 | 69079 | 68882 | CspA | 8.34e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MBEMHNKK_5 | 299177 | 299371 | CspB | 6.95e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| MBEMHNKK_9 | 69079 | 68885 | CspR | 1.09e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| MBEMHNKK_4 | 227189 | 228061 | lipA (SAUSA300_0829) | 1.15e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| MBEMHNKK_15 | 1902 | 2088 | Col(SD853) | 187 / 194 | 86.63 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MBEMHNKK_1 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_2 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_3 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_4 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_5 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_6 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_7 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_8 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_9 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_10 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_11 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_12 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_13 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_14 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_15 | 1.0 | 0.0 | 1.0 |
| MBEMHNKK_16 | 0.0 | 1.0 | 0.0 |
| MBEMHNKK_17 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MBEMHNKK_1 | 936613 | 961264 | False | Siphoviridae | VOG0275, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896 |
| MBEMHNKK_2 | 697097 | 722157 | False | Myoviridae | VOG0753, VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG5660, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)