Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 32 | 40.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PKNEEJFP_125 | 1588 | 2181 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| PKNEEJFP_79 | 185 | 1645 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 99.93 | 100.00 | WP_011681177 |
| PKNEEJFP_125 | 1588 | 2181 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| PKNEEJFP_111 | 186107 | 186916 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_002948020 |
| PKNEEJFP_125 | 2181 | 3050 | rfbA | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011681286 |
| PKNEEJFP_125 | 1588 | 2181 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| PKNEEJFP_104 | 101428 | 103086 | fbp54 | 0.0 | FBPs | Adherence | 99.64 | 100.00 | WP_011227179 |
| PKNEEJFP_111 | 185167 | 186123 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011226328 |
| PKNEEJFP_111 | 189205 | 190989 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.44 | 100.00 | WP_011226325 |
| PKNEEJFP_111 | 186916 | 188121 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.17 | 100.00 | WP_011226326 |
| PKNEEJFP_111 | 178129 | 178980 | rfbD | 0.0 | Capsule | Immune modulation | 99.06 | 100.00 | WP_014621820 |
| PKNEEJFP_79 | 1646 | 2377 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011681176 |
| PKNEEJFP_42 | 46571 | 47539 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.86 | 100.00 | WP_082302295 |
| PKNEEJFP_89 | 24898 | 25659 | srtA | 0.0 | Sortase A | Exoenzyme | 98.82 | 100.00 | - |
| PKNEEJFP_111 | 184022 | 185170 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_041828290 |
| PKNEEJFP_79 | 1646 | 2377 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011227248 |
| PKNEEJFP_79 | 1646 | 2377 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.36 | 100.00 | WP_011226048 |
| PKNEEJFP_56 | 1350 | 2143 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.24 | 100.25 | - |
| PKNEEJFP_79 | 12394 | 13809 | STER_RS05250 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_011681165 |
| PKNEEJFP_79 | 3088 | 3828 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.11 | 100.00 | WP_011226046 |
| PKNEEJFP_79 | 3088 | 3828 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011681174 |
| PKNEEJFP_79 | 185 | 1645 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.40 | 100.21 | WP_011226049 |
| PKNEEJFP_79 | 3088 | 3823 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.15 | 98.13 | WP_011227246 |
| PKNEEJFP_79 | 3885 | 5252 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.00 | 100.00 | WP_011226045 |
| PKNEEJFP_79 | 185 | 1645 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.85 | 100.21 | WP_041827043 |
| PKNEEJFP_79 | 2386 | 3078 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 95.38 | 100.00 | WP_011227247 |
| PKNEEJFP_79 | 2386 | 3078 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 95.38 | 100.00 | WP_011226047 |
| PKNEEJFP_111 | 188141 | 189208 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 95.27 | 101.14 | WP_002948022 |
| PKNEEJFP_79 | 3885 | 5250 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.58 | 99.85 | WP_011681173 |
| PKNEEJFP_79 | 2386 | 3078 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.48 | 100.00 | WP_011681175 |
| PKNEEJFP_111 | 180083 | 181357 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 89.42 | 99.92 | WP_011226331 |
| PKNEEJFP_13 | 96006 | 97309 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| PKNEEJFP_21 | 262 | 1272 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.54 | 99.41 | WP_002986042 |
| PKNEEJFP_10 | 59470 | 60753 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.81 | 99.77 | WP_000107753 |
| PKNEEJFP_125 | 113 | 1159 | rfbB | 0.0 | Capsule | Immune modulation | 80.61 | 100.00 | WP_009659423 |
| PKNEEJFP_79 | 15629 | 16212 | SPD_RS01775 | 9.71e-154 | Capsule | Immune modulation | 83.59 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PKNEEJFP_89 | 22211 | 21228 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| PKNEEJFP_32 | 59454 | 60527 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| PKNEEJFP_111 | 15572 | 13269 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| PKNEEJFP_13 | 12368 | 13336 | spr0084 | 0.0 | pathogenitcity related | 92.57 | 98.48 | AAK98888 |
| PKNEEJFP_72 | 14125 | 16371 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| PKNEEJFP_91 | 17269 | 15788 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.70 | 100.00 | ABJ54480 |
| PKNEEJFP_71 | 39649 | 40647 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| PKNEEJFP_71 | 3535 | 4506 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| PKNEEJFP_111 | 129589 | 130740 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| PKNEEJFP_111 | 164952 | 166940 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| PKNEEJFP_89 | 38410 | 40140 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| PKNEEJFP_41 | 27387 | 28865 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| PKNEEJFP_13 | 96006 | 97307 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| PKNEEJFP_41 | 42576 | 44468 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| PKNEEJFP_72 | 19296 | 17587 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| PKNEEJFP_78 | 18063 | 19073 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| PKNEEJFP_104 | 79574 | 80935 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| PKNEEJFP_78 | 68407 | 66515 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| PKNEEJFP_13 | 81570 | 82460 | bglB | 3.36e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| PKNEEJFP_81 | 33376 | 34314 | SP_0676 | 5.66e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| PKNEEJFP_72 | 16563 | 17420 | metF | 2.06e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| PKNEEJFP_56 | 29823 | 30527 | covR | 1.57e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| PKNEEJFP_118 | 18231 | 18902 | rr01 | 7.49e-126 | response regulator | 83.93 | 99.56 | CAB54566 |
| PKNEEJFP_56 | 29823 | 30527 | walR | 6.60e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| PKNEEJFP_29 | 16855 | 17541 | CovR | 1.33e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| PKNEEJFP_72 | 23250 | 23918 | CiaR | 2.73e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| PKNEEJFP_72 | 23250 | 23921 | ciaR | 2.69e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| PKNEEJFP_71 | 40923 | 41297 | SpxA2 | 2.58e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| PKNEEJFP_104 | 68212 | 67811 | SpxA1 | 2.35e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| PKNEEJFP_37 | 25830 | 25543 | spr0479 | 3.01e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| PKNEEJFP_42 | 5689 | 5435 | spr1327 | 1.88e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| PKNEEJFP_68 | 20927 | 20664 | spr0175 | 8.78e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| PKNEEJFP_68 | 20100 | 19834 | spr0177 | 1.92e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| PKNEEJFP_111 | 15572 | 13275 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| PKNEEJFP_78 | 53947 | 52928 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PKNEEJFP_1 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_5 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_6 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_10 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_13 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_16 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_17 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_21 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_29 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_32 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_33 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_36 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_37 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_39 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_41 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_42 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_47 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_51 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_56 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_60 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_62 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_65 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_67 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_68 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_69 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_70 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_71 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_72 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_73 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_76 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_78 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_79 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_80 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_81 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_82 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_83 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_84 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_86 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_89 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_90 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_91 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_93 | 1.0 | 0.0 | 1.0 |
| PKNEEJFP_94 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_96 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_97 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_102 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_104 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_109 | 1.0 | 0.0 | 1.0 |
| PKNEEJFP_111 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_118 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_121 | 0.0 | 1.0 | 0.0 |
| PKNEEJFP_125 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)