safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
IHPIBCPB_1	1131671	1132264	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
IHPIBCPB_1	1005354	1006814	STER_RS05320	0.0	Capsule	Immune modulation	99.93	100.00	WP_011681177
IHPIBCPB_1	1131671	1132264	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
IHPIBCPB_1	1344317	1345126	STU_RS16245	0.0	Capsule	Immune modulation	99.75	100.00	WP_002948020
IHPIBCPB_1	1132264	1133133	rfbA	0.0	Capsule	Immune modulation	99.66	100.00	WP_011681286
IHPIBCPB_1	1131671	1132264	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
IHPIBCPB_1	885391	887049	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
IHPIBCPB_1	1345110	1346066	STU_RS16250	0.0	Capsule	Immune modulation	99.58	100.00	WP_011226328
IHPIBCPB_1	43670	44509	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
IHPIBCPB_1	1340244	1342028	STU_RS16230	0.0	Capsule	Immune modulation	99.44	100.00	WP_011226325
IHPIBCPB_1	1343112	1344317	STU_RS16240	0.0	Capsule	Immune modulation	99.17	100.00	WP_011226326
IHPIBCPB_1	1352253	1353104	rfbD	0.0	Capsule	Immune modulation	99.06	100.00	WP_014621820
IHPIBCPB_1	1004622	1005353	STER_RS05315	0.0	Capsule	Immune modulation	98.91	100.00	WP_011681176
IHPIBCPB_1	1168457	1169218	srtA	0.0	Sortase A	Exoenzyme	98.82	100.00	-
IHPIBCPB_1	1346063	1347211	STU_RS16255	0.0	Capsule	Immune modulation	98.78	100.00	WP_041828290
IHPIBCPB_1	1004622	1005353	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
IHPIBCPB_1	1004622	1005353	STU_RS14645	0.0	Capsule	Immune modulation	98.36	100.00	WP_011226048
IHPIBCPB_1	1072696	1073489	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.24	100.25	-
IHPIBCPB_1	993190	994605	STER_RS05250	0.0	Capsule	Immune modulation	98.23	100.00	WP_011681165
IHPIBCPB_1	1003171	1003911	STU_RS14635	0.0	Capsule	Immune modulation	97.98	100.00	WP_011226046
IHPIBCPB_1	1003171	1003911	STER_RS05305	0.0	Capsule	Immune modulation	97.57	100.00	WP_011681174
IHPIBCPB_1	1005354	1006814	STU_RS14650	0.0	Capsule	Immune modulation	97.40	100.21	WP_011226049
IHPIBCPB_1	1003176	1003911	STR_RS05250	0.0	Capsule	Immune modulation	97.01	98.13	WP_011227246
IHPIBCPB_1	1001747	1003114	STU_RS14630	0.0	Capsule	Immune modulation	97.00	100.00	WP_011226045
IHPIBCPB_1	1005354	1006814	STR_RS05265	0.0	Capsule	Immune modulation	96.85	100.21	WP_041827043
IHPIBCPB_1	1003921	1004613	STR_RS05255	0.0	Capsule	Immune modulation	95.38	100.00	WP_011227247
IHPIBCPB_1	1003921	1004613	STU_RS14640	0.0	Capsule	Immune modulation	95.38	100.00	WP_011226047
IHPIBCPB_1	1342025	1343092	STU_RS16235	0.0	Capsule	Immune modulation	95.27	101.14	WP_002948022
IHPIBCPB_1	1001749	1003114	STER_RS05300	0.0	Capsule	Immune modulation	94.58	99.85	WP_011681173
IHPIBCPB_1	1003921	1004613	STER_RS05310	0.0	Capsule	Immune modulation	90.48	100.00	WP_011681175
IHPIBCPB_1	1349876	1351150	STU_RS16265	0.0	Capsule	Immune modulation	89.42	99.92	WP_011226331
IHPIBCPB_1	604685	605988	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.46	WP_000022832
IHPIBCPB_1	1638641	1639651	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
IHPIBCPB_1	145707	146990	tig/ropA	0.0	Trigger factor	Stress survival	80.81	99.77	WP_000107753
IHPIBCPB_1	1130196	1131242	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
IHPIBCPB_1	990787	991370	SPD_RS01775	1.09e-151	Capsule	Immune modulation	83.59	96.20	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
IHPIBCPB_1	1171905	1172888	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
IHPIBCPB_1	723956	725029	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
IHPIBCPB_1	1515661	1517964	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
IHPIBCPB_1	521047	522015	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
IHPIBCPB_1	752276	754522	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
IHPIBCPB_1	1242217	1243698	SPD_0310	0.0	UPF0371 protein SPD_0310	91.70	100.00	ABJ54480
IHPIBCPB_1	63301	64299	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
IHPIBCPB_1	27187	28158	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
IHPIBCPB_1	1401644	1400493	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
IHPIBCPB_1	1366281	1364293	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
IHPIBCPB_1	1155706	1153976	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
IHPIBCPB_1	1845681	1844203	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
IHPIBCPB_1	604685	605986	Eno	0.0	enolase	86.21	99.77	ABP90468
IHPIBCPB_1	1830492	1828600	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
IHPIBCPB_1	757447	755738	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
IHPIBCPB_1	1682039	1683049	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
IHPIBCPB_1	910224	908863	mnmE	0.0	central tRNA-modifying GTPase	82.02	99.56	ABP90420
IHPIBCPB_1	1732383	1730491	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
IHPIBCPB_1	590249	591139	bglB	5.26e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
IHPIBCPB_1	830294	829356	SP_0676	2.36e-167	transcription facot	81.15	97.20	AAK74821
IHPIBCPB_1	754714	755571	metF	1.53e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
IHPIBCPB_1	1045016	1044312	covR	4.39e-131	responder protein	85.53	100.00	AAK71319
IHPIBCPB_1	1223242	1222571	rr01	8.31e-124	response regulator	83.93	99.56	CAB54566
IHPIBCPB_1	1045016	1044312	walR	1.85e-121	DNA-binding response regulator	82.13	100.00	ANC99504
IHPIBCPB_1	298737	299423	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
IHPIBCPB_1	761401	762069	CiaR	2.02e-117	two-component response regulator	84.31	99.55	AAK74935
IHPIBCPB_1	761401	762072	ciaR	1.99e-115	two component system response regulator	82.14	99.12	AIK75708
IHPIBCPB_1	64575	64949	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
IHPIBCPB_1	921586	921987	SpxA1	1.48e-65	transcriptional regulator	80.60	100.00	ERL19273
IHPIBCPB_1	147148	147648	RopE (M5005_Spy_1611)	4.93e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
IHPIBCPB_1	1515661	1517958	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
IHPIBCPB_1	1717923	1716904	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IHPIBCPB_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found