safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
DFKCLBBC_1	1122323	1123192	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
DFKCLBBC_1	1121730	1122323	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
DFKCLBBC_1	1339231	1340040	STU_RS16245	Capsule	Immune modulation	99.88	100.00	WP_002948020
DFKCLBBC_1	1121730	1122323	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
DFKCLBBC_1	1340024	1340980	STU_RS16250	Capsule	Immune modulation	99.79	100.00	WP_011226328
DFKCLBBC_1	1340977	1342125	STU_RS16255	Capsule	Immune modulation	99.74	100.00	WP_041828290
DFKCLBBC_1	1121730	1122323	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
DFKCLBBC_1	1345621	1346472	rfbD	Capsule	Immune modulation	99.65	100.00	WP_014621820
DFKCLBBC_1	913818	915476	fbp54	FBPs	Adherence	99.58	100.00	WP_011227179
DFKCLBBC_1	1338026	1339231	STU_RS16240	Capsule	Immune modulation	99.58	100.00	WP_011226326
DFKCLBBC_1	1335170	1336954	STU_RS16230	Capsule	Immune modulation	99.50	100.00	WP_011226325
DFKCLBBC_1	1342234	1343244	STU_RS16260	Capsule	Immune modulation	99.50	100.00	WP_011226330
DFKCLBBC_1	1336951	1338006	STU_RS16235	Capsule	Immune modulation	99.34	100.00	WP_002948022
DFKCLBBC_1	819778	821193	STER_RS05250	Capsule	Immune modulation	99.08	100.00	WP_011681165
DFKCLBBC_1	1158513	1159274	srtA	Sortase A	Exoenzyme	98.82	100.00	-
DFKCLBBC_1	43734	44573	cbpD	Autolysin	Exoenzyme	98.69	100.00	WP_011226733
DFKCLBBC_1	1062657	1063445	cshA	Cell surface hydrophobicity proteins	Adherence	98.48	99.75	-
DFKCLBBC_1	794872	795603	STU_RS14645	Capsule	Immune modulation	98.36	100.00	WP_011226048
DFKCLBBC_1	794872	795603	STER_RS05315	Capsule	Immune modulation	98.09	100.00	WP_011681176
DFKCLBBC_1	794872	795603	STR_RS05260	Capsule	Immune modulation	97.68	100.00	WP_011227248
DFKCLBBC_1	1343247	1344518	STU_RS16265	Capsule	Immune modulation	97.48	99.76	WP_011226331
DFKCLBBC_1	793411	794871	STER_RS05320	Capsule	Immune modulation	97.06	100.00	WP_011681177
DFKCLBBC_1	796314	797054	STER_RS05305	Capsule	Immune modulation	97.03	100.00	WP_011681174
DFKCLBBC_1	796314	797054	STU_RS14635	Capsule	Immune modulation	96.36	100.00	WP_011226046
DFKCLBBC_1	793411	794871	STU_RS14650	Capsule	Immune modulation	96.30	100.21	WP_011226049
DFKCLBBC_1	793411	794871	STR_RS05265	Capsule	Immune modulation	96.17	100.21	WP_041827043
DFKCLBBC_1	796314	797049	STR_RS05250	Capsule	Immune modulation	96.06	98.13	WP_011227246
DFKCLBBC_1	797111	798474	STU_RS14630	Capsule	Immune modulation	95.53	99.71	WP_011226045
DFKCLBBC_1	795612	796304	STR_RS05255	Capsule	Immune modulation	95.25	99.86	WP_011227247
DFKCLBBC_1	795612	796304	STU_RS14640	Capsule	Immune modulation	95.25	99.86	WP_011226047
DFKCLBBC_1	797111	798478	STER_RS05300	Capsule	Immune modulation	94.52	99.93	WP_011681173
DFKCLBBC_1	795612	796304	STER_RS05310	Capsule	Immune modulation	89.32	100.00	WP_011681175
DFKCLBBC_1	600976	602279	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
DFKCLBBC_1	1633455	1634465	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	87.36	99.80	WP_002986042
DFKCLBBC_1	142298	143581	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
DFKCLBBC_1	1120255	1121301	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
DFKCLBBC_1	1161961	1162944	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
DFKCLBBC_1	708559	709632	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
DFKCLBBC_1	1511439	1513742	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
DFKCLBBC_1	517341	518309	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
DFKCLBBC_1	1236622	1238103	SPD_0310	0.0	UPF0371 protein SPD_0310	92.31	100.00	ABJ54480
DFKCLBBC_1	736937	739183	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
DFKCLBBC_1	63364	64356	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
DFKCLBBC_1	27248	28219	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
DFKCLBBC_1	1397746	1396595	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
DFKCLBBC_1	1361442	1359454	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
DFKCLBBC_1	1145766	1144036	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
DFKCLBBC_1	1837483	1836005	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.42	100.00	ADE32434
DFKCLBBC_1	600976	602277	Eno	0.0	enolase	86.21	99.77	ABP90468
DFKCLBBC_1	1822294	1820402	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
DFKCLBBC_1	1039023	1040732	PGM	0.0	phosphoglucomutase	84.06	99.65	ACH87041
DFKCLBBC_1	1676746	1677756	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
DFKCLBBC_1	885922	887283	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
DFKCLBBC_1	1727113	1725221	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
DFKCLBBC_1	586540	587430	bglB	5.24e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
DFKCLBBC_1	969281	970216	SP_0676	7.37e-168	transcription facot	81.73	96.89	AAK74821
DFKCLBBC_1	739375	740232	metF	1.53e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
DFKCLBBC_1	756556	757260	covR	4.37e-131	responder protein	85.53	100.00	AAK71319
DFKCLBBC_1	1217647	1216976	rr01	3.00e-124	response regulator	83.93	99.56	CAB54566
DFKCLBBC_1	756556	757260	walR	1.84e-121	DNA-binding response regulator	82.13	100.00	ANC99504
DFKCLBBC_1	297978	298664	CovR	1.02e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
DFKCLBBC_1	1035069	1034401	CiaR	2.02e-117	two-component response regulator	84.31	99.55	AAK74935
DFKCLBBC_1	1035069	1034398	ciaR	1.98e-115	two component system response regulator	82.14	99.12	AIK75708
DFKCLBBC_1	64653	65048	SpxA2	3.69e-74	transcriptional regulator	90.91	100.00	ERL21461
DFKCLBBC_1	874559	874158	SpxA1	3.75e-66	transcriptional regulator	80.60	100.00	ERL19273
DFKCLBBC_1	143739	144239	RopE (M5005_Spy_1611)	4.91e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
DFKCLBBC_1	1511439	1513736	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
DFKCLBBC_1	1712657	1711638	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
DFKCLBBC_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found