Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 9 | 17.38 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KKOACABF_2 | 253296 | 254216 | mphK | ARO:3004541 | mphK | 99.35 | 100.00 | CP010314.1 |
| KKOACABF_2 | 239964 | 241403 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.42 | JYFL01000006.1 |
| KKOACABF_2 | 190253 | 190846 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| KKOACABF_4 | 84230 | 84547 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KKOACABF_4 | 83892 | 84230 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KKOACABF_6 | 59210 | 60379 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KKOACABF_19 | 29136 | 30782 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| KKOACABF_12 | 27757 | 30327 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| KKOACABF_1 | 4853 | 5707 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KKOACABF_2 | 253296 | 254216 | mph(K) | Spiramycin, Telithromycin | 99.46 | 100.00 | NC_000964 |
| KKOACABF_1 | 4853 | 5707 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KKOACABF_2 | 253299 | 254216 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.35 | 100.00 | WP_003246254.1 |
| KKOACABF_19 | 29136 | 30779 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| KKOACABF_1 | 4856 | 5707 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| KKOACABF_18 | 38221 | 38739 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| KKOACABF_28 | 3426 | 6023 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KKOACABF_25 | 24601 | 25782 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| KKOACABF_3 | 292499 | 293284 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| KKOACABF_17 | 9694 | 10335 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| KKOACABF_3 | 293310 | 294506 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| KKOACABF_23 | 41744 | 43507 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| KKOACABF_3 | 296182 | 297120 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| KKOACABF_25 | 22976 | 24118 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| KKOACABF_25 | 24137 | 24586 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| KKOACABF_3 | 297140 | 304276 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.42 | 100.00 | NP_391076 |
| KKOACABF_3 | 294535 | 296154 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KKOACABF_22 | 28047 | 26911 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KKOACABF_30 | 11248 | 10379 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KKOACABF_13 | 93859 | 94635 | CodY | 1.10e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KKOACABF_3 | 29896 | 29327 | ClpP | 1.98e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| KKOACABF_11 | 35688 | 35296 | spxA1 | 8.81e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| KKOACABF_11 | 35688 | 35296 | spx | 5.26e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| KKOACABF_24 | 34773 | 34531 | SpoVG | 6.15e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| KKOACABF_38 | 391 | 194 | CspA | 3.25e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| KKOACABF_14 | 128932 | 128738 | CspD | 8.02e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| KKOACABF_8 | 133958 | 134152 | CspB | 1.39e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KKOACABF_1 | 0.0 | 1.0 | 0.0 |
| KKOACABF_2 | 0.0 | 1.0 | 0.0 |
| KKOACABF_3 | 0.0 | 1.0 | 0.0 |
| KKOACABF_4 | 0.0 | 1.0 | 0.0 |
| KKOACABF_5 | 0.0 | 1.0 | 0.0 |
| KKOACABF_6 | 0.0 | 1.0 | 0.0 |
| KKOACABF_7 | 0.0 | 1.0 | 0.0 |
| KKOACABF_8 | 0.0 | 1.0 | 0.0 |
| KKOACABF_9 | 0.0 | 1.0 | 0.0 |
| KKOACABF_10 | 0.0 | 1.0 | 0.0 |
| KKOACABF_11 | 0.0 | 1.0 | 0.0 |
| KKOACABF_12 | 0.0 | 1.0 | 0.0 |
| KKOACABF_13 | 0.0 | 1.0 | 0.0 |
| KKOACABF_14 | 0.0 | 1.0 | 0.0 |
| KKOACABF_15 | 0.0 | 1.0 | 0.0 |
| KKOACABF_16 | 0.0 | 1.0 | 0.0 |
| KKOACABF_17 | 0.0 | 1.0 | 0.0 |
| KKOACABF_18 | 0.0 | 1.0 | 0.0 |
| KKOACABF_19 | 0.0 | 1.0 | 0.0 |
| KKOACABF_20 | 0.0 | 1.0 | 0.0 |
| KKOACABF_21 | 0.0 | 1.0 | 0.0 |
| KKOACABF_22 | 0.0 | 1.0 | 0.0 |
| KKOACABF_23 | 0.0 | 1.0 | 0.0 |
| KKOACABF_24 | 0.0 | 1.0 | 0.0 |
| KKOACABF_25 | 0.0 | 1.0 | 0.0 |
| KKOACABF_26 | 0.0 | 1.0 | 0.0 |
| KKOACABF_27 | 0.0 | 1.0 | 0.0 |
| KKOACABF_28 | 0.0 | 1.0 | 0.0 |
| KKOACABF_29 | 0.0 | 1.0 | 0.0 |
| KKOACABF_30 | 0.0 | 1.0 | 0.0 |
| KKOACABF_31 | 0.0 | 1.0 | 0.0 |
| KKOACABF_32 | 0.0 | 1.0 | 0.0 |
| KKOACABF_33 | 0.0 | 1.0 | 0.0 |
| KKOACABF_34 | 0.0 | 1.0 | 0.0 |
| KKOACABF_35 | 0.0 | 1.0 | 0.0 |
| KKOACABF_36 | 0.0 | 1.0 | 0.0 |
| KKOACABF_37 | 0.0 | 1.0 | 0.0 |
| KKOACABF_38 | 1.0 | 0.0 | 1.0 |
| KKOACABF_39 | 0.0 | 1.0 | 0.0 |
| KKOACABF_40 | 0.0 | 1.0 | 0.0 |
| KKOACABF_41 | 1.0 | 0.0 | 1.0 |
| KKOACABF_42 | 0.0 | 1.0 | 0.0 |
| KKOACABF_43 | 0.0 | 1.0 | 0.0 |
| KKOACABF_44 | 0.0 | 1.0 | 0.0 |
| KKOACABF_45 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KKOACABF_4 | 28029 | 54341 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| KKOACABF_15 | 27017 | 31420 | False | Unknown | VOG6211, VOG0322, VOG7476 |
| KKOACABF_17 | 65440 | 66937 | False | Unknown | VOG7930, VOG8610, VOG7895 |
| KKOACABF_17 | 68587 | 74310 | False | Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG9867 |
Phigaro v2.4.0 (Database: Jan. 2024 release)