Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIPBMJPJ_1 | 3195931 | 3196785 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| DIPBMJPJ_1 | 3178850 | 3180052 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DIPBMJPJ_1 | 3161585 | 3162439 | aadK | ARO:3002627 | aadK | 88.73 | 100.00 | AL009126.1 |
| DIPBMJPJ_1 | 2975768 | 2976937 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DIPBMJPJ_1 | 1958450 | 1958767 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DIPBMJPJ_1 | 1958112 | 1958450 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DIPBMJPJ_1 | 1497503 | 1500073 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| DIPBMJPJ_1 | 1195199 | 1196845 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| DIPBMJPJ_1 | 945102 | 945695 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| DIPBMJPJ_1 | 894607 | 896040 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| DIPBMJPJ_1 | 881793 | 882713 | mphK | ARO:3004541 | mphK | 99.01 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DIPBMJPJ_1 | 881793 | 882713 | mph(K) | Spiramycin, Telithromycin | 99.13 | 100.00 | NC_000964 |
| DIPBMJPJ_1 | 3195931 | 3196785 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIPBMJPJ_1 | 1195202 | 1196845 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| DIPBMJPJ_1 | 881793 | 882701 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.01 | 99.02 | WP_003246254.1 |
| DIPBMJPJ_1 | 3195934 | 3196785 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| DIPBMJPJ_1 | 580618 | 581136 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| DIPBMJPJ_1 | 3161588 | 3162439 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 88.73 | 100.00 | WP_003229862.1 |
| DIPBMJPJ_1 | 2680861 | 2683458 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DIPBMJPJ_1 | 2778455 | 2779096 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| DIPBMJPJ_1 | 3740094 | 3740879 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| DIPBMJPJ_1 | 88337 | 89518 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| DIPBMJPJ_1 | 3738872 | 3740068 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| DIPBMJPJ_1 | 87873 | 88322 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| DIPBMJPJ_1 | 86712 | 87854 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| DIPBMJPJ_1 | 2626643 | 2628406 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| DIPBMJPJ_1 | 3729102 | 3736238 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.43 | 100.00 | NP_391076 |
| DIPBMJPJ_1 | 3736258 | 3737196 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| DIPBMJPJ_1 | 3737224 | 3738843 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DIPBMJPJ_1 | 742243 | 743379 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DIPBMJPJ_1 | 628510 | 629379 | pdxS | 3.03e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DIPBMJPJ_1 | 2269860 | 2270636 | CodY | 3.31e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DIPBMJPJ_1 | 4015999 | 4016568 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DIPBMJPJ_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DIPBMJPJ_1 | 1901517 | 1928599 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG9315, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DIPBMJPJ_1 | 2471723 | 2497497 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG7896, VOG0641 |
Phigaro v2.4.0 (Database: Jan. 2024 release)