Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EPKEEPDC_1 | 4115874 | 4117043 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| EPKEEPDC_1 | 3087278 | 3087595 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EPKEEPDC_1 | 3086940 | 3087278 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EPKEEPDC_1 | 2622008 | 2624578 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.42 | 100.00 | AL009126.3 |
| EPKEEPDC_1 | 2310111 | 2311757 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| EPKEEPDC_1 | 2031405 | 2031998 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| EPKEEPDC_1 | 1980905 | 1982338 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| EPKEEPDC_1 | 1968090 | 1969010 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| EPKEEPDC_1 | 220335 | 221189 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| EPKEEPDC_1 | 202039 | 203241 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EPKEEPDC_1 | 1968090 | 1969010 | mph(K) | Spiramycin, Telithromycin | 99.02 | 100.00 | NC_000964 |
| EPKEEPDC_1 | 220335 | 221189 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EPKEEPDC_1 | 1968090 | 1968998 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| EPKEEPDC_1 | 2310114 | 2311757 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| EPKEEPDC_1 | 220338 | 221189 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| EPKEEPDC_1 | 1670350 | 1670868 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| EPKEEPDC_1 | 3814340 | 3816937 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EPKEEPDC_1 | 1186467 | 1187648 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| EPKEEPDC_1 | 3918343 | 3918984 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| EPKEEPDC_1 | 777894 | 778679 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| EPKEEPDC_1 | 774058 | 774996 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| EPKEEPDC_1 | 1186003 | 1186452 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| EPKEEPDC_1 | 3768652 | 3770415 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| EPKEEPDC_1 | 1184843 | 1185984 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 99.91 | NP_391469 |
| EPKEEPDC_1 | 766902 | 774038 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391076 |
| EPKEEPDC_1 | 776672 | 777868 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.41 | 100.00 | NP_391079 |
| EPKEEPDC_1 | 775024 | 776643 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EPKEEPDC_1 | 1833552 | 1834688 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EPKEEPDC_1 | 1719814 | 1720683 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EPKEEPDC_1 | 3398919 | 3399695 | CodY | 3.36e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EPKEEPDC_1 | 1028298 | 1028867 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EPKEEPDC_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EPKEEPDC_1 | 146677 | 164411 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749 |
| EPKEEPDC_1 | 3029814 | 3057388 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| EPKEEPDC_1 | 3599449 | 3623908 | False | Siphoviridae | VOG0198, VOG0275, VOG6879, VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG7896 |
Phigaro v2.4.0 (Database: Jan. 2024 release)