safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MEHKOANC_1	2786700	2787554	aadK	ARO:3002627	aadK	97.54	100.00	AL009126.1
MEHKOANC_1	2767531	2768733	blt	ARO:3003006	blt	99.75	100.00	L32599.1
MEHKOANC_1	2539755	2540924	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MEHKOANC_1	1333064	1333381	ykkD	ARO:3003064	ykkD	99.05	100.00	AL009126.1
MEHKOANC_1	1332726	1333064	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MEHKOANC_1	869647	872217	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	99.88	100.00	AL009126.3
MEHKOANC_1	578337	579983	vmlR	ARO:3004476	vmlR	98.54	100.18	NC_000964.3
MEHKOANC_1	337299	337892	tmrB	ARO:3003059	tmrB	98.48	100.00	AL009126.3
MEHKOANC_1	286739	288172	lmrB	ARO:3002813	lmrB	99.58	100.00	JYFL01000006.1
MEHKOANC_1	273926	274846	mphK	ARO:3004541	mphK	98.37	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MEHKOANC_1	4262200	4263576	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034
MEHKOANC_1	2786702	2787554	aadK	Streptomycin	98.12	99.77	M26879
MEHKOANC_1	273926	274846	mph(K)	Spiramycin, Telithromycin	97.94	100.00	NC_000964

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MEHKOANC_1	4262203	4263576	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
MEHKOANC_1	578340	579983	vmlR	ABC-F type ribosomal protection protein VmlR	BLASTX	98.54	100.00	WP_003234144.1
MEHKOANC_1	273926	274843	mphK	macrolide 2'-phosphotransferase MphK	BLASTX	98.37	100.00	WP_003246254.1
MEHKOANC_1	2786703	2787554	aadK	aminoglycoside 6-adenylyltransferase AadK	BLASTX	97.54	100.00	WP_003229862.1
MEHKOANC_1	4259729	4260247	satA	streptothricin N-acetyltransferase SatA	BLASTX	90.17	100.00	WP_003242546.1
MEHKOANC_1	2072792	2075389	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.40	100.00	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MEHKOANC_1	2340587	2341228	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062
MEHKOANC_1	3765782	3766963	capB	Capsule	Immune modulation	99.75	100.00	NP_391471
MEHKOANC_1	3764157	3765299	capA	Capsule	Immune modulation	99.39	100.00	NP_391469
MEHKOANC_1	3347696	3348634	dhbB	Bacillibactin	Nutritional/Metabolic factor	99.36	100.00	NP_391077
MEHKOANC_1	3765318	3765767	capC	Capsule	Immune modulation	99.33	100.00	NP_391470
MEHKOANC_1	3351532	3352317	dhbA	Bacillibactin	Nutritional/Metabolic factor	99.24	100.00	NP_391080
MEHKOANC_1	3350310	3351506	dhbC	Bacillibactin	Nutritional/Metabolic factor	99.17	100.00	NP_391079
MEHKOANC_1	2022712	2024475	dep/capD	Capsule	Immune modulation	99.15	100.00	NP_389723
MEHKOANC_1	3340540	3347676	dhbF	Bacillibactin	Nutritional/Metabolic factor	99.05	100.00	NP_391076
MEHKOANC_1	3348662	3350281	dhbE	Bacillibactin	Nutritional/Metabolic factor	98.52	100.00	NP_391078

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MEHKOANC_1	132400	133536	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MEHKOANC_1	18666	19535	pdxS	3.19e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MEHKOANC_1	1643211	1643987	CodY	3.48e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
MEHKOANC_1	3604438	3605007	ClpP	8.73e-98	part of proteolytic complex	80.53	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MEHKOANC_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MEHKOANC_1	1275343	1303238	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705
MEHKOANC_1	1845621	1874684	False	Siphoviridae	VOG6809, VOG0198, VOG11015, VOG0713, VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG4705, VOG5221, VOG0136, VOG4666, VOG6495
MEHKOANC_1	2100758	2123487	False	Siphoviridae	VOG4972, VOG0136, VOG5221, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG9029
MEHKOANC_1	2288248	2307309	False	Unknown	VOG0275, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG7900, VOG10230
MEHKOANC_1	2718259	2740315	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773

Phigaro v2.4.0 (Database: Jan. 2024 release)