Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 9 | 18.03 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LHFEEFLP_2 | 637200 | 638369 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| LHFEEFLP_1 | 528858 | 529196 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| LHFEEFLP_1 | 528541 | 528858 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| LHFEEFLP_2 | 453078 | 454280 | blt | ARO:3003006 | blt | 98.50 | 100.00 | L32599.1 |
| LHFEEFLP_2 | 434127 | 434981 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| LHFEEFLP_4 | 351770 | 352690 | mphK | ARO:3004541 | mphK | 98.04 | 100.00 | CP010314.1 |
| LHFEEFLP_4 | 338437 | 339870 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| LHFEEFLP_4 | 288712 | 289305 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| LHFEEFLP_4 | 28916 | 30544 | vmlR | ARO:3004476 | vmlR | 95.38 | 99.09 | NC_000964.3 |
| LHFEEFLP_6 | 27455 | 30025 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LHFEEFLP_2 | 434127 | 434981 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
| LHFEEFLP_4 | 351770 | 352690 | mph(K) | Spiramycin, Telithromycin | 97.39 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LHFEEFLP_4 | 351773 | 352690 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.04 | 100.00 | WP_003246254.1 |
| LHFEEFLP_2 | 434127 | 434978 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| LHFEEFLP_4 | 28916 | 30538 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.38 | 98.90 | WP_003234144.1 |
| LHFEEFLP_5 | 327284 | 327802 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| LHFEEFLP_7 | 148078 | 150675 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.29 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LHFEEFLP_3 | 537352 | 538533 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| LHFEEFLP_3 | 536888 | 537337 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| LHFEEFLP_3 | 535727 | 536869 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| LHFEEFLP_3 | 113218 | 114003 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.98 | 100.00 | NP_391080 |
| LHFEEFLP_7 | 240768 | 241409 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.75 | 100.00 | NP_390062 |
| LHFEEFLP_3 | 109382 | 110320 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.72 | 100.00 | NP_391077 |
| LHFEEFLP_3 | 111996 | 113192 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391079 |
| LHFEEFLP_3 | 102232 | 109362 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.32 | 99.99 | NP_391076 |
| LHFEEFLP_7 | 103134 | 104897 | dep/capD | 0.0 | Capsule | Immune modulation | 98.02 | 100.00 | NP_389723 |
| LHFEEFLP_3 | 110348 | 111967 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.59 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LHFEEFLP_10 | 31783 | 32919 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| LHFEEFLP_12 | 4340 | 5209 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| LHFEEFLP_1 | 219563 | 218787 | CodY | 7.22e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LHFEEFLP_3 | 380852 | 381421 | ClpP | 4.27e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| LHFEEFLP_1 | 677184 | 676792 | spxA1 | 5.67e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| LHFEEFLP_1 | 677184 | 676792 | spx | 3.38e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| LHFEEFLP_11 | 34670 | 34428 | SpoVG | 7.50e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| LHFEEFLP_4 | 83977 | 83780 | CspA | 1.92e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| LHFEEFLP_2 | 823800 | 823606 | CspD | 5.10e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| LHFEEFLP_9 | 32076 | 31882 | CspB | 5.66e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LHFEEFLP_1 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_2 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_3 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_4 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_5 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_6 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_7 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_8 | 1.0 | 0.0 | 1.0 |
| LHFEEFLP_9 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_10 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_11 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_12 | 0.0 | 1.0 | 0.0 |
| LHFEEFLP_14 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LHFEEFLP_1 | 559030 | 585166 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)