Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KDFNNOCA_1 | 2565741 | 2566595 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| KDFNNOCA_1 | 2536217 | 2537419 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| KDFNNOCA_1 | 2360560 | 2361729 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| KDFNNOCA_1 | 1376961 | 1377278 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| KDFNNOCA_1 | 1376623 | 1376961 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| KDFNNOCA_1 | 915892 | 918462 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| KDFNNOCA_1 | 613860 | 615506 | vmlR | ARO:3004476 | vmlR | 99.27 | 100.18 | NC_000964.3 |
| KDFNNOCA_1 | 337627 | 338220 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| KDFNNOCA_1 | 287072 | 288505 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| KDFNNOCA_1 | 274260 | 275180 | mphK | ARO:3004541 | mphK | 96.73 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KDFNNOCA_1 | 4006856 | 4008232 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.42 | 100.00 | D12567 |
| KDFNNOCA_1 | 2565741 | 2566595 | aadK | Streptomycin | 98.48 | 100.00 | M26879 |
| KDFNNOCA_1 | 274260 | 275180 | mph(K) | Spiramycin, Telithromycin | 96.85 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KDFNNOCA_1 | 4006859 | 4008232 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.34 | 100.00 | WP_063856081.1 |
| KDFNNOCA_1 | 613863 | 615506 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.27 | 100.00 | WP_003234144.1 |
| KDFNNOCA_1 | 2565744 | 2566595 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| KDFNNOCA_1 | 274260 | 275177 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.73 | 100.00 | WP_003246254.1 |
| KDFNNOCA_1 | 4004328 | 4004846 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 92.49 | 100.00 | WP_003242546.1 |
| KDFNNOCA_1 | 2055041 | 2057638 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.94 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KDFNNOCA_1 | 3118064 | 3119002 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| KDFNNOCA_1 | 3529633 | 3530814 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| KDFNNOCA_1 | 3119030 | 3120649 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.63 | 100.00 | NP_391078 |
| KDFNNOCA_1 | 3121900 | 3122685 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| KDFNNOCA_1 | 3528008 | 3529150 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| KDFNNOCA_1 | 3110908 | 3118044 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.27 | 100.00 | NP_391076 |
| KDFNNOCA_1 | 2156967 | 2157608 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| KDFNNOCA_1 | 3529169 | 3529618 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| KDFNNOCA_1 | 2009202 | 2010965 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| KDFNNOCA_1 | 3120678 | 3121874 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KDFNNOCA_1 | 132399 | 133535 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| KDFNNOCA_1 | 18666 | 19535 | pdxS | 3.00e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KDFNNOCA_1 | 1690373 | 1691149 | CodY | 3.28e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KDFNNOCA_1 | 3375697 | 3376266 | ClpP | 2.43e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KDFNNOCA_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KDFNNOCA_1 | 1319693 | 1346775 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)