Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 4 | 14.70 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GNKBLEDE_1 | 2505187 | 2506041 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| GNKBLEDE_1 | 2488090 | 2489292 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| GNKBLEDE_1 | 1383301 | 1383618 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| GNKBLEDE_1 | 1382963 | 1383301 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| GNKBLEDE_1 | 891438 | 894008 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| GNKBLEDE_1 | 596615 | 598261 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| GNKBLEDE_1 | 327492 | 328085 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| GNKBLEDE_1 | 278441 | 279874 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| GNKBLEDE_1 | 265626 | 266546 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GNKBLEDE_1 | 2505187 | 2506041 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| GNKBLEDE_1 | 265626 | 266546 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GNKBLEDE_1 | 265626 | 266534 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| GNKBLEDE_1 | 2505190 | 2506041 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| GNKBLEDE_1 | 596618 | 598261 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| GNKBLEDE_1 | 4082486 | 4083004 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GNKBLEDE_1 | 3575851 | 3577032 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| GNKBLEDE_1 | 3061506 | 3062291 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| GNKBLEDE_1 | 3574226 | 3575368 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| GNKBLEDE_1 | 3050514 | 3057650 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| GNKBLEDE_1 | 1962220 | 1963983 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| GNKBLEDE_1 | 3057670 | 3058608 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| GNKBLEDE_1 | 3060284 | 3061480 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| GNKBLEDE_1 | 2093126 | 2093767 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| GNKBLEDE_1 | 3575387 | 3575836 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| GNKBLEDE_1 | 3058636 | 3060255 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GNKBLEDE_1 | 132417 | 133553 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GNKBLEDE_1 | 18600 | 19469 | pdxS | 5.35e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| GNKBLEDE_1 | 1693710 | 1694486 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GNKBLEDE_1 | 3418475 | 3419044 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GNKBLEDE_3 | 571 | 738 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GNKBLEDE_1 | 0.0 | 1.0 | 0.0 |
| GNKBLEDE_2 | 1.0 | 0.0 | 1.0 |
| GNKBLEDE_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GNKBLEDE_1 | 1324498 | 1353499 | False | Myoviridae | VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4843, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| GNKBLEDE_1 | 1819120 | 1849181 | False | Siphoviridae | VOG4552, VOG0650, VOG0535, VOG1936, VOG1227, VOG4606, VOG2752, VOG7372, VOG0198, VOG4841, VOG7403, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG0136 |
| GNKBLEDE_1 | 3255287 | 3293814 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
| GNKBLEDE_1 | 3384134 | 3395292 | False | Siphoviridae | VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801 |
| GNKBLEDE_1 | 4019443 | 4028122 | False | Unknown | VOG6422, VOG3239, VOG7443, VOG0944, VOG5532 |
Phigaro v2.4.0 (Database: Jan. 2024 release)