safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
5	1	0	5.10

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
NFJKBKGD_1	4221622	4222524	CepA-44	ARO:3006225	CepA-44	99.33	100.00	NG_050386.1
NFJKBKGD_1	4159474	4160208	ErmG	ARO:3000522	ErmG	98.77	100.00	L42817.1
NFJKBKGD_1	3851867	3853792	tet(Q)	ARO:3000191	tet(Q)	97.04	97.56	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
NFJKBKGD_1	3851867	3853792	tet(Q)	Doxycycline, Tetracycline, Minocycline	100.00	100.00	X58717
NFJKBKGD_1	4221622	4222524	cepA	Unknown Beta-lactam	99.89	100.00	U05887
NFJKBKGD_1	4159474	4160208	erm(G)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.18	100.00	M15332
NFJKBKGD_1	4157239	4158702	msr(D)	Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S	97.54	100.00	AF274302
NFJKBKGD_1	4155899	4157113	mef(A)	Erythromycin, Azithromycin	94.24	99.75	AF227520

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
NFJKBKGD_1	3851867	3853789	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	EXACTX	100.00	100.00	WP_004293868.1
NFJKBKGD_1	4221625	4222524	cepA	CepA family extended-spectrum class A beta-lactamase	BLASTX	99.67	100.00	WP_005816369.1
NFJKBKGD_1	4159474	4160205	erm(G)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(G)	BLASTX	99.18	100.00	WP_063844791.1
NFJKBKGD_1	4157239	4158699	msr(D)	ABC-F type ribosomal protection protein Msr(D)	BLASTX	97.13	100.00	WP_000420313.1
NFJKBKGD_1	4155899	4157122	mef(A)	macrolide efflux MFS transporter Mef(A)	BLASTX	96.57	100.00	WP_012102963.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
NFJKBKGD_1	2670205	2671398	bft-2	BFT B. fragilis toxin, subtype 2	Exotoxin	100.00	100.00	BAA77277
NFJKBKGD_1	2670205	2671398	bft-3	BFT B. fragilis toxin, subtype 3	Exotoxin	96.90	100.00	BAA77275
NFJKBKGD_1	2670205	2671398	bft-1	BFT B. fragilis toxin, subtype 1	Exotoxin	94.89	100.00	BAA77276

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
NFJKBKGD_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
NFJKBKGD_1	869999	881582	False	Siphoviridae	VOG4581,

Phigaro v2.4.0 (Database: Jan. 2024 release)