Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 1 | 4 | 5.74 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PIKALECD_93 | 38177 | 39229 | clbC | ARO:3002816 | clbC | 97.71 | 100.00 | AP006627.1 |
| PIKALECD_104 | 24726 | 25496 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 93.75 | 100.00 | EF540343.1 |
| PIKALECD_56 | 678 | 1523 | Erm(34) | ARO:3000600 | Erm(34) | 100.00 | 100.00 | AY234334.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| PIKALECD_56 | 678 | 1523 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AY234334 |
| PIKALECD_104 | 24726 | 25496 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 94.29 | 100.00 | AJ506108 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| PIKALECD_56 | 681 | 1523 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | EXACTX | 100.00 | 100.00 | WP_063844504.1 |
| PIKALECD_93 | 38180 | 39229 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 97.71 | 100.00 | WP_011245929.1 |
| PIKALECD_104 | 21410 | 22312 | blaBCL | BCL family class A beta-lactamase | BLASTX | 97.34 | 100.00 | WP_065825670.1 |
| PIKALECD_104 | 24729 | 25496 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 93.75 | 100.00 | WP_035202393.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| PIKALECD_49 | 332069 | 332719 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 97.39 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| PIKALECD_47 | 33361 | 34503 | tufA | 0.0 | elongation factor Tu | 87.14 | 96.19 | AHM69299 |
| PIKALECD_45 | 36887 | 37129 | SpoVG | 2.59e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| PIKALECD_62 | 1735 | 1932 | CspA | 6.05e-31 | cold shock protein | 86.36 | 100.00 | CAA62903 |
| PIKALECD_46 | 54697 | 54885 | CspB | 9.50e-26 | cold shock protein | 82.54 | 95.45 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| PIKALECD_11 | 0.0 | 1.0 | 0.0 |
| PIKALECD_22 | 0.0 | 1.0 | 0.0 |
| PIKALECD_33 | 0.0 | 1.0 | 0.0 |
| PIKALECD_42 | 0.0 | 1.0 | 0.0 |
| PIKALECD_43 | 0.0 | 1.0 | 0.0 |
| PIKALECD_44 | 0.0 | 1.0 | 0.0 |
| PIKALECD_45 | 0.0 | 1.0 | 0.0 |
| PIKALECD_46 | 0.0 | 1.0 | 0.0 |
| PIKALECD_47 | 0.0 | 1.0 | 0.0 |
| PIKALECD_48 | 0.0 | 1.0 | 0.0 |
| PIKALECD_49 | 0.0 | 1.0 | 0.0 |
| PIKALECD_50 | 0.0 | 1.0 | 0.0 |
| PIKALECD_51 | 0.0 | 1.0 | 0.0 |
| PIKALECD_52 | 0.0 | 1.0 | 0.0 |
| PIKALECD_53 | 0.0 | 1.0 | 0.0 |
| PIKALECD_54 | 0.0 | 1.0 | 0.0 |
| PIKALECD_55 | 0.0 | 1.0 | 0.0 |
| PIKALECD_56 | 0.0 | 1.0 | 0.0 |
| PIKALECD_57 | 0.0 | 1.0 | 0.0 |
| PIKALECD_58 | 0.0 | 1.0 | 0.0 |
| PIKALECD_59 | 0.0 | 1.0 | 0.0 |
| PIKALECD_60 | 0.0 | 1.0 | 0.0 |
| PIKALECD_61 | 0.0 | 1.0 | 0.0 |
| PIKALECD_62 | 0.0 | 1.0 | 0.0 |
| PIKALECD_63 | 0.0 | 1.0 | 0.0 |
| PIKALECD_64 | 0.0 | 1.0 | 0.0 |
| PIKALECD_65 | 0.0 | 1.0 | 0.0 |
| PIKALECD_66 | 0.0 | 1.0 | 0.0 |
| PIKALECD_67 | 0.0 | 1.0 | 0.0 |
| PIKALECD_68 | 1.0 | 0.0 | 1.0 |
| PIKALECD_69 | 0.0 | 1.0 | 0.0 |
| PIKALECD_70 | 0.0 | 1.0 | 0.0 |
| PIKALECD_71 | 0.0 | 1.0 | 0.0 |
| PIKALECD_73 | 1.0 | 0.0 | 1.0 |
| PIKALECD_74 | 1.0 | 0.0 | 1.0 |
| PIKALECD_75 | 1.0 | 0.0 | 1.0 |
| PIKALECD_76 | 0.0 | 1.0 | 0.0 |
| PIKALECD_77 | 0.0 | 1.0 | 0.0 |
| PIKALECD_79 | 1.0 | 0.0 | 1.0 |
| PIKALECD_81 | 1.0 | 0.0 | 1.0 |
| PIKALECD_82 | 0.0 | 1.0 | 0.0 |
| PIKALECD_83 | 1.0 | 0.0 | 1.0 |
| PIKALECD_93 | 0.0 | 1.0 | 0.0 |
| PIKALECD_104 | 0.0 | 1.0 | 0.0 |
| PIKALECD_115 | 0.0 | 1.0 | 0.0 |
| PIKALECD_126 | 0.0 | 1.0 | 0.0 |
| PIKALECD_137 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)