Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 10 | 10 | 9 | 16.76 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ACMMGGEE_101 | 206329 | 206922 | tmrB | ARO:3003059 | tmrB | 99.49 | 100.00 | AL009126.3 |
| ACMMGGEE_64 | 61079 | 62281 | blt | ARO:3003006 | blt | 98.00 | 100.00 | L32599.1 |
| ACMMGGEE_64 | 44330 | 45184 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| ACMMGGEE_63 | 40969 | 41889 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| ACMMGGEE_33 | 40711 | 41049 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| ACMMGGEE_33 | 40394 | 40711 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| ACMMGGEE_63 | 27635 | 29074 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| ACMMGGEE_44 | 27446 | 30016 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.53 | 100.00 | AL009126.3 |
| ACMMGGEE_87 | 5730 | 7376 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ACMMGGEE_64 | 44330 | 45184 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| ACMMGGEE_63 | 40969 | 41889 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ACMMGGEE_63 | 40981 | 41889 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| ACMMGGEE_64 | 44330 | 45181 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| ACMMGGEE_87 | 5733 | 7376 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| ACMMGGEE_89 | 10859 | 11377 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ACMMGGEE_68 | 398242 | 399423 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ACMMGGEE_134 | 114388 | 115173 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ACMMGGEE_68 | 399906 | 401048 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| ACMMGGEE_134 | 103396 | 110532 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| ACMMGGEE_96 | 19804 | 21567 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| ACMMGGEE_134 | 110552 | 111490 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.04 | 100.00 | NP_391077 |
| ACMMGGEE_134 | 113166 | 114362 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ACMMGGEE_112 | 143947 | 144588 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| ACMMGGEE_68 | 399438 | 399887 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| ACMMGGEE_134 | 111518 | 113137 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 96.73 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ACMMGGEE_70 | 28135 | 26999 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ACMMGGEE_98 | 11278 | 10409 | pdxS | 1.96e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| ACMMGGEE_90 | 96973 | 96197 | CodY | 2.01e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ACMMGGEE_80 | 4454 | 5023 | ClpP | 2.96e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| ACMMGGEE_66 | 41557 | 41165 | spxA1 | 4.13e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ACMMGGEE_66 | 41557 | 41165 | spx | 2.46e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| ACMMGGEE_76 | 34758 | 34516 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| ACMMGGEE_77 | 40527 | 40330 | CspA | 2.35e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| ACMMGGEE_112 | 132717 | 132523 | CspD | 1.09e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| ACMMGGEE_156 | 85831 | 86025 | CspB | 1.07e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| ACMMGGEE_110 | 3608 | 3775 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ACMMGGEE_11 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_22 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_33 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_44 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_55 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_63 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_64 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_65 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_66 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_67 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_68 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_69 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_70 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_71 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_72 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_73 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_74 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_75 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_76 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_77 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_78 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_79 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_80 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_81 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_82 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_83 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_84 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_85 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_86 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_87 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_88 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_89 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_90 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_91 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_92 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_93 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_94 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_95 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_96 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_97 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_98 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_99 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_100 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_101 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_102 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_103 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_104 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_105 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_106 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_107 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_108 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_109 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_110 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_111 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_112 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_113 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_114 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_115 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_116 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_117 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_118 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_119 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_120 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_121 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_122 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_123 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_124 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_125 | 1.0 | 0.0 | 1.0 |
| ACMMGGEE_126 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_134 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_145 | 0.0 | 1.0 | 0.0 |
| ACMMGGEE_156 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ACMMGGEE_33 | 70513 | 97196 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490 |
| ACMMGGEE_78 | 2 | 25888 | False | Siphoviridae | VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG7403, VOG4841, VOG0198, VOG7372, VOG2752, VOG4606, VOG1227, VOG1936, VOG0535, VOG0650 |
| ACMMGGEE_79 | 169060 | 207587 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)