Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 11 | 7 | 17.72 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HANOOMAO_30 | 1655583 | 1656437 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| HANOOMAO_30 | 1626683 | 1627885 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| HANOOMAO_30 | 1430124 | 1431293 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| HANOOMAO_30 | 439993 | 440310 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| HANOOMAO_30 | 439655 | 439993 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HANOOMAO_73 | 351086 | 352729 | vmlR | ARO:3004476 | vmlR | 98.17 | 100.00 | NC_000964.3 |
| HANOOMAO_84 | 252165 | 254735 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| HANOOMAO_73 | 98476 | 99069 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| HANOOMAO_73 | 47919 | 49358 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| HANOOMAO_73 | 35107 | 35973 | mphK | ARO:3004541 | mphK | 98.61 | 94.12 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HANOOMAO_30 | 1655585 | 1656437 | aadK | Streptomycin | 98.94 | 99.77 | M26879 |
| HANOOMAO_73 | 35107 | 36027 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HANOOMAO_30 | 1655586 | 1656437 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| HANOOMAO_73 | 351089 | 352729 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.17 | 100.00 | WP_003234144.1 |
| HANOOMAO_73 | 35107 | 36024 | mphK | macrolide 2'-phosphotransferase MphK | INTERNAL_STOP | 98.04 | 100.00 | WP_003246254.1 |
| HANOOMAO_41 | 535702 | 536220 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.33 | 100.00 | WP_003242546.1 |
| HANOOMAO_30 | 1116124 | 1118721 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HANOOMAO_41 | 50391 | 51572 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| HANOOMAO_52 | 109316 | 110254 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| HANOOMAO_52 | 102160 | 109296 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.58 | 100.00 | NP_391076 |
| HANOOMAO_41 | 49927 | 50376 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| HANOOMAO_52 | 111930 | 113126 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.42 | 100.00 | NP_391079 |
| HANOOMAO_41 | 48766 | 49908 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| HANOOMAO_30 | 1230578 | 1231219 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| HANOOMAO_52 | 113152 | 113937 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| HANOOMAO_30 | 1065819 | 1067582 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| HANOOMAO_52 | 110282 | 111901 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.64 | 100.00 | NP_391078 |
| HANOOMAO_88 | 1 | 431 | BPS_RS00060 | 9.11e-130 | Gsp | Effector delivery system | 86.17 | 71.17 | WP_004535320 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HANOOMAO_106 | 28140 | 27004 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| HANOOMAO_23 | 11291 | 10422 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| HANOOMAO_30 | 750499 | 751275 | CodY | 1.39e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HANOOMAO_52 | 361249 | 361818 | ClpP | 2.77e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| HANOOMAO_30 | 275620 | 276012 | spxA1 | 1.10e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| HANOOMAO_30 | 275620 | 276012 | spx | 6.53e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| HANOOMAO_117 | 34797 | 34555 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HANOOMAO_73 | 300196 | 300393 | CspA | 1.65e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HANOOMAO_11 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_21 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_22 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_23 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_24 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_25 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_27 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_28 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_29 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_30 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_31 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_32 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_34 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_35 | 1.0 | 0.0 | 1.0 |
| HANOOMAO_41 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_52 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_62 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_73 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_84 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_95 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_106 | 0.0 | 1.0 | 0.0 |
| HANOOMAO_117 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HANOOMAO_21 | 10289 | 35675 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG9667, VOG9667, VOG0650, VOG4552, VOG4603, VOG4600, VOG1309, VOG4566, VOG0189, VOG4693, VOG7365, VOG4694, VOG4567, VOG0198, VOG0713, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586 |
| HANOOMAO_30 | 382614 | 410152 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| HANOOMAO_62 | 274845 | 279678 | False | Myoviridae | VOG4600, VOG1309, VOG5263, VOG0189, VOG0044 |
| HANOOMAO_62 | 284918 | 310458 | False | Myoviridae | VOG0796, VOG10227, VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0641, VOG4574, VOG6422, VOG6116 |
| HANOOMAO_62 | 437108 | 447183 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541 |
Phigaro v2.4.0 (Database: Jan. 2024 release)