safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
EHCBDFOD_1	37745	38449	WalR	1.72e-113	transcriptional regulatory protein	84.25	100.00	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
EHCBDFOD_1	0.0	1.0	0.0
EHCBDFOD_2	1.0	0.0	1.0
EHCBDFOD_3	1.0	0.0	1.0
EHCBDFOD_4	1.0	0.0	1.0
EHCBDFOD_5	1.0	0.0	1.0
EHCBDFOD_6	1.0	0.0	1.0
EHCBDFOD_7	1.0	0.0	1.0
EHCBDFOD_8	1.0	0.0	1.0
EHCBDFOD_9	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
EHCBDFOD_1	975776	992727	False	Siphoviridae	VOG0044, VOG0198, VOG4841, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG6163, VOG4605, VOG4599, VOG10957
EHCBDFOD_1	1346280	1367419	False	Siphoviridae	VOG4841, VOG4153, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG5904, VOG4605, VOG4599, VOG10957, VOG10957
EHCBDFOD_1	2096662	2144915	False	Siphoviridae	VOG0054, VOG1637, VOG8294, VOG0703, VOG5008, VOG9531, VOG4619, VOG0801, VOG0701, VOG9763, VOG0698, VOG0697, VOG0696, VOG0695, VOG4572, VOG4555, VOG0692, VOG0691, VOG4544, VOG0796, VOG0996, VOG0198, VOG0044, VOG11003, VOG5039, VOG4693, VOG4566, VOG0226, VOG0283, VOG9667, VOG0181, VOG3622, VOG9667, VOG4602, VOG1521, VOG1347, VOG6317, VOG0275, VOG0198, VOG0204, VOG4568, VOG4556, VOG2935, VOG4544, VOG4581, VOG0205, VOG6414
EHCBDFOD_1	2164018	2191840	False	Siphoviridae	VOG8294, VOG0703, VOG8438, VOG4705, VOG0804, VOG9640, VOG4856, VOG4619, VOG4828, VOG4633, VOG1472, VOG0725, VOG0799, VOG0724, VOG9583, VOG4713, VOG1329, VOG4555, VOG4564, VOG0720, VOG10227, VOG0796, VOG0996, VOG0198, VOG3643
EHCBDFOD_2	5161	18609	False	Siphoviridae	VOG4552, VOG0181, VOG0535, VOG0322, VOG0514, VOG0514, VOG10904, VOG8647, VOG4605, VOG4605, VOG4605, VOG4605, VOG4599, VOG4626
EHCBDFOD_4	5079	32876	False	Siphoviridae	VOG4581, VOG1886, VOG5051, VOG4556, VOG4568, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG0660, VOG5904, VOG4605, VOG4605, VOG5904, VOG4605, VOG4599, VOG10957
EHCBDFOD_5	51755	54401	False	Unknown	VOG3624, VOG6396, VOG9404