Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFKIPINL_1 | 2589738 | 2590592 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| MFKIPINL_1 | 2571024 | 2572226 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| MFKIPINL_1 | 2362528 | 2363697 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| MFKIPINL_1 | 1349140 | 1349457 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| MFKIPINL_1 | 1348802 | 1349140 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| MFKIPINL_1 | 882738 | 885308 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.42 | 100.00 | AL009126.3 |
| MFKIPINL_1 | 592049 | 593692 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| MFKIPINL_1 | 344580 | 345173 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| MFKIPINL_1 | 294058 | 295491 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| MFKIPINL_1 | 281244 | 282164 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MFKIPINL_1 | 4062232 | 4063608 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.98 | 100.00 | X08034 |
| MFKIPINL_1 | 2589738 | 2590592 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| MFKIPINL_1 | 281244 | 282164 | mph(K) | Spiramycin, Telithromycin | 98.26 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MFKIPINL_1 | 4062235 | 4063608 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_003242953.1 |
| MFKIPINL_1 | 592052 | 593692 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| MFKIPINL_1 | 281244 | 282161 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| MFKIPINL_1 | 2589741 | 2590592 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| MFKIPINL_1 | 4059773 | 4060291 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| MFKIPINL_1 | 2055516 | 2058113 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MFKIPINL_1 | 3141534 | 3142472 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| MFKIPINL_1 | 2163117 | 2163758 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| MFKIPINL_1 | 3558311 | 3559492 | capB | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | NP_391471 |
| MFKIPINL_1 | 3134378 | 3141514 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391076 |
| MFKIPINL_1 | 3142500 | 3144119 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.51 | 100.00 | NP_391078 |
| MFKIPINL_1 | 3145370 | 3146155 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| MFKIPINL_1 | 3144148 | 3145344 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| MFKIPINL_1 | 3557847 | 3558296 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| MFKIPINL_1 | 3556686 | 3557828 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| MFKIPINL_1 | 2005218 | 2006981 | dep/capD | 0.0 | Capsule | Immune modulation | 98.70 | 100.00 | NP_389723 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MFKIPINL_1 | 132404 | 133540 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| MFKIPINL_1 | 18665 | 19534 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| MFKIPINL_1 | 1688524 | 1689300 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MFKIPINL_1 | 3398658 | 3399227 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MFKIPINL_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MFKIPINL_1 | 169237 | 169769 | False | Unknown | VOG9667 |
| MFKIPINL_1 | 1291802 | 1317844 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)