Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 2 | 9 | 9.70 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JHFHBKII_91 | 52329 | 53378 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| JHFHBKII_91 | 52343 | 53378 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.61 | 98.67 | KR610408 |
| JHFHBKII_64 | 372264 | 373640 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.87 | 99.93 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| JHFHBKII_91 | 52332 | 53378 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| JHFHBKII_64 | 372264 | 373634 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 87.31 | 99.78 | WP_003242953.1 |
| JHFHBKII_64 | 371533 | 372051 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 80.35 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JHFHBKII_1 | 515002 | 516183 | capB | 0.0 | Capsule | Immune modulation | 82.21 | 99.66 | NP_391471 |
| JHFHBKII_1 | 514538 | 514987 | capC | 3.75e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JHFHBKII_22 | 31684 | 32820 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| JHFHBKII_50 | 4316 | 5185 | pdxS | 1.37e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| JHFHBKII_53 | 284554 | 285330 | CodY | 7.37e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| JHFHBKII_82 | 251580 | 251972 | spxA1 | 6.28e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| JHFHBKII_91 | 73197 | 73000 | CspA | 1.30e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| JHFHBKII_74 | 128700 | 128894 | CspD | 1.97e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| JHFHBKII_82 | 35769 | 35575 | CspB | 5.98e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| JHFHBKII_91 | 73197 | 73003 | CspR | 1.70e-26 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| JHFHBKII_1 | 138043 | 137171 | lipA (SAUSA300_0829) | 3.25e-162 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
| JHFHBKII_73 | 62 | 1183 | BcactA (Bcin16g02020) | 0.0 | conserved actin protein | 87.43 | 99.73 | ATZ58410 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JHFHBKII_1 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_2 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_11 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_22 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_32 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_43 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_48 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_49 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_50 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_51 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_52 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_53 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_54 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_55 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_56 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_58 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_61 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_63 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_64 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_65 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_70 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_73 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_74 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_79 | 1.0 | 0.0 | 1.0 |
| JHFHBKII_82 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_91 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_102 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_113 | 0.0 | 1.0 | 0.0 |
| JHFHBKII_124 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| JHFHBKII_2 | 60745 | 88037 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG7947, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895 |
Phigaro v2.4.0 (Database: Jan. 2024 release)