Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HFDALOLN_1 | 2594639 | 2595493 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
| HFDALOLN_1 | 2575471 | 2576673 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| HFDALOLN_1 | 2350686 | 2351855 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| HFDALOLN_1 | 1356228 | 1356545 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| HFDALOLN_1 | 1355890 | 1356228 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| HFDALOLN_1 | 892699 | 895269 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| HFDALOLN_1 | 590270 | 591913 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| HFDALOLN_1 | 337275 | 337868 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| HFDALOLN_1 | 286753 | 288186 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| HFDALOLN_1 | 273937 | 274857 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HFDALOLN_1 | 4059522 | 4060898 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| HFDALOLN_1 | 2594641 | 2595493 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| HFDALOLN_1 | 273937 | 274857 | mph(K) | Spiramycin, Telithromycin | 97.50 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HFDALOLN_1 | 4059525 | 4060898 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| HFDALOLN_1 | 590273 | 591913 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| HFDALOLN_1 | 273937 | 274854 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| HFDALOLN_1 | 2594642 | 2595493 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| HFDALOLN_1 | 4057063 | 4057581 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| HFDALOLN_1 | 2046710 | 2049307 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HFDALOLN_1 | 3573741 | 3574922 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| HFDALOLN_1 | 1996685 | 1998448 | dep/capD | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_389723 |
| HFDALOLN_1 | 2151279 | 2151920 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| HFDALOLN_1 | 3159250 | 3160035 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| HFDALOLN_1 | 3155414 | 3156352 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| HFDALOLN_1 | 3158028 | 3159224 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| HFDALOLN_1 | 3148260 | 3155394 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.06 | 99.97 | NP_391076 |
| HFDALOLN_1 | 3572116 | 3573258 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| HFDALOLN_1 | 3156380 | 3157999 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
| HFDALOLN_1 | 3573277 | 3573726 | capC | 0.0 | Capsule | Immune modulation | 98.44 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HFDALOLN_1 | 132397 | 133533 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| HFDALOLN_1 | 18664 | 19533 | pdxS | 7.07e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| HFDALOLN_1 | 1666122 | 1666898 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| HFDALOLN_1 | 3412388 | 3412957 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HFDALOLN_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HFDALOLN_1 | 1298416 | 1326079 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| HFDALOLN_1 | 2523354 | 2541701 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685 |
Phigaro v2.4.0 (Database: Jan. 2024 release)