Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 9 | 18.71 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GBLIBGMN_2 | 274212 | 275132 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| GBLIBGMN_2 | 260878 | 262317 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| GBLIBGMN_2 | 211221 | 211814 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| GBLIBGMN_9 | 136091 | 138661 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
| GBLIBGMN_6 | 115623 | 115961 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| GBLIBGMN_6 | 115306 | 115623 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| GBLIBGMN_18 | 47355 | 48998 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| GBLIBGMN_4 | 36866 | 37720 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| GBLIBGMN_8 | 34629 | 35798 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| GBLIBGMN_4 | 18157 | 19359 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GBLIBGMN_4 | 36866 | 37720 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| GBLIBGMN_2 | 274212 | 275132 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| GBLIBGMN_3 | 36380 | 37756 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GBLIBGMN_4 | 36869 | 37720 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| GBLIBGMN_2 | 274215 | 275132 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| GBLIBGMN_3 | 39756 | 40274 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| GBLIBGMN_3 | 36380 | 37753 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| GBLIBGMN_18 | 47358 | 48998 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| GBLIBGMN_17 | 12563 | 15157 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GBLIBGMN_1 | 200167 | 201309 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| GBLIBGMN_1 | 201792 | 202973 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| GBLIBGMN_1 | 201328 | 201777 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| GBLIBGMN_17 | 60052 | 61815 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| GBLIBGMN_27 | 22284 | 23069 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| GBLIBGMN_27 | 25967 | 26905 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| GBLIBGMN_27 | 23095 | 24291 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| GBLIBGMN_27 | 24320 | 25939 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| GBLIBGMN_27 | 26925 | 34061 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| GBLIBGMN_35 | 8500 | 9141 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GBLIBGMN_32 | 9745 | 10881 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| GBLIBGMN_45 | 4231 | 5100 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| GBLIBGMN_7 | 35421 | 34645 | CodY | 1.37e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GBLIBGMN_1 | 55573 | 56142 | ClpP | 2.66e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| GBLIBGMN_28 | 2325 | 1933 | spxA1 | 9.88e-71 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| GBLIBGMN_23 | 34575 | 34333 | SpoVG | 7.48e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| GBLIBGMN_18 | 1883 | 2080 | CspA | 3.37e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| GBLIBGMN_29 | 37714 | 37520 | CspD | 2.52e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| GBLIBGMN_16 | 56442 | 56636 | CspB | 8.04e-28 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GBLIBGMN_1 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_2 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_3 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_4 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_5 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_6 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_7 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_8 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_9 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_10 | 1.0 | 0.0 | 1.0 |
| GBLIBGMN_11 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_12 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_13 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_14 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_15 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_16 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_17 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_18 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_19 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_20 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_21 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_22 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_23 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_24 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_25 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_26 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_27 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_28 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_29 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_30 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_31 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_32 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_33 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_34 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_35 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_36 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_37 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_38 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_39 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_40 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_41 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_42 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_43 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_44 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_45 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_46 | 1.0 | 0.0 | 1.0 |
| GBLIBGMN_47 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_48 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_49 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_50 | 1.0 | 0.0 | 1.0 |
| GBLIBGMN_51 | 0.0 | 1.0 | 0.0 |
| GBLIBGMN_52 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GBLIBGMN_6 | 145800 | 171908 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| GBLIBGMN_10 | 7155 | 45569 | False | Myoviridae | VOG7724, VOG5721, VOG6031, VOG6524, VOG6246, VOG6941, VOG6036, VOG6472, VOG7629, VOG7628, VOG6940, VOG5534, VOG8610, VOG8609, VOG0275, VOG3389, VOG5242, VOG9244, VOG3432, VOG5173, VOG0647, VOG7230, VOG4615, VOG9091, VOG0703, VOG4714 |
| GBLIBGMN_10 | 96988 | 105981 | False | Myoviridae / Siphoviridae | VOG0154, VOG0982, VOG0677, VOG0918, VOG5263, VOG4551, VOG0025, VOG7627 |
| GBLIBGMN_24 | 1950 | 12368 | False | Myoviridae | VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)