Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 1 | 2 | 8 | 8.31 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LNBFJIIK_13 | 87232 | 88281 | clbA | ARO:3002814 | clbA | 99.71 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LNBFJIIK_13 | 87232 | 88267 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 98.67 | KR610408 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LNBFJIIK_13 | 87232 | 88278 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.71 | 100.00 | WP_012116915.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LNBFJIIK_3 | 26740 | 27921 | capB | 0.0 | Capsule | Immune modulation | 81.96 | 99.66 | NP_391471 |
| LNBFJIIK_3 | 27936 | 28385 | capC | 6.54e-118 | Capsule | Immune modulation | 84.03 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LNBFJIIK_19 | 38125 | 39261 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| LNBFJIIK_23 | 4275 | 5144 | pdxS | 1.34e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| LNBFJIIK_6 | 235083 | 235859 | CodY | 3.86e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LNBFJIIK_4 | 175810 | 176202 | spxA1 | 8.04e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| LNBFJIIK_13 | 64826 | 65023 | CspA | 5.46e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| LNBFJIIK_5 | 351663 | 351469 | CspD | 9.32e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| LNBFJIIK_17 | 33066 | 32872 | CspB | 1.50e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| LNBFJIIK_13 | 64826 | 65020 | CspR | 7.16e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| LNBFJIIK_8 | 9835 | 10707 | lipA (SAUSA300_0829) | 4.50e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LNBFJIIK_1 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_2 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_3 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_4 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_5 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_6 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_7 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_8 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_9 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_10 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_11 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_12 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_13 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_14 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_15 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_16 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_17 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_18 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_19 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_20 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_21 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_22 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_23 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_24 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_25 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_26 | 0.0 | 1.0 | 0.0 |
| LNBFJIIK_29 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LNBFJIIK_11 | 70073 | 96187 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG3390, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)