safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	35	28	44.82

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
AELLADNN_1	879614	880483	rfbA	Capsule	Immune modulation	100.00	100.00	WP_011681286
AELLADNN_1	1025029	1026444	STER_RS05250	Capsule	Immune modulation	100.00	100.00	WP_011681165
AELLADNN_1	1022848	1023828	STER_RS05260	Capsule	Immune modulation	100.00	100.00	WP_011681166
AELLADNN_1	1021671	1022855	STER_RS05265	Capsule	Immune modulation	100.00	100.00	WP_011681167
AELLADNN_1	1020602	1021654	STER_RS05270	Capsule	Immune modulation	100.00	100.00	WP_011681168
AELLADNN_1	1019679	1020587	STER_RS05275	Capsule	Immune modulation	100.00	100.00	WP_011681169
AELLADNN_1	1018597	1019658	STER_RS05280	Capsule	Immune modulation	100.00	100.00	WP_202964935
AELLADNN_1	1018069	1018560	STER_RS05285	Capsule	Immune modulation	100.00	100.00	WP_011681171
AELLADNN_1	1017581	1018057	STER_RS05290	Capsule	Immune modulation	100.00	100.00	WP_011681172
AELLADNN_1	1014661	1015401	STER_RS05305	Capsule	Immune modulation	100.00	100.00	WP_011681174
AELLADNN_1	1013959	1014651	STER_RS05310	Capsule	Immune modulation	100.00	100.00	WP_011681175
AELLADNN_1	1013219	1013950	STER_RS05315	Capsule	Immune modulation	100.00	100.00	WP_011681176
AELLADNN_1	1011758	1013218	STER_RS05320	Capsule	Immune modulation	100.00	100.00	WP_011681177
AELLADNN_1	880483	881076	STER_RS06030	Capsule	Immune modulation	100.00	100.00	WP_011226149
AELLADNN_1	665669	667414	STER_RS07045	Capsule	Immune modulation	100.00	100.00	WP_011681434
AELLADNN_1	663967	665667	STER_RS07050	Capsule	Immune modulation	100.00	100.00	WP_041827753
AELLADNN_1	662747	663949	STER_RS07055	Capsule	Immune modulation	100.00	100.00	WP_011681436
AELLADNN_1	661941	662747	STER_RS07060	Capsule	Immune modulation	100.00	100.00	WP_011681437
AELLADNN_1	661000	661941	STER_RS07065	Capsule	Immune modulation	100.00	100.00	WP_011681438
AELLADNN_1	659855	661003	STER_RS07070	Capsule	Immune modulation	100.00	100.00	WP_011681439
AELLADNN_1	658960	659736	STER_RS07075	Capsule	Immune modulation	100.00	100.00	WP_011681440
AELLADNN_1	657898	658614	STER_RS07085	Capsule	Immune modulation	100.00	100.00	WP_011681442
AELLADNN_1	656929	657897	STER_RS07090	Capsule	Immune modulation	100.00	100.00	WP_011681443
AELLADNN_1	655934	656911	STER_RS07095	Capsule	Immune modulation	100.00	100.00	WP_011681444
AELLADNN_1	654678	655937	STER_RS07100	Capsule	Immune modulation	100.00	100.00	WP_011681445
AELLADNN_1	1015458	1016825	STER_RS05300	Capsule	Immune modulation	99.85	100.00	WP_011681173
AELLADNN_1	880483	881076	STR_RS05880	Capsule	Immune modulation	99.83	100.00	WP_011226149
AELLADNN_1	663967	665667	STR_RS06890	Capsule	Immune modulation	99.71	100.00	WP_041827077
AELLADNN_1	880483	881076	STU_RS15260	Capsule	Immune modulation	99.66	100.00	WP_011226149
AELLADNN_1	1117890	1119548	fbp54	FBPs	Adherence	99.64	100.00	WP_011227179
AELLADNN_1	104398	105237	cbpD	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
AELLADNN_1	661941	662747	STR_RS06900	Capsule	Immune modulation	99.50	100.00	WP_011227429
AELLADNN_1	661000	661941	STR_RS06905	Capsule	Immune modulation	99.47	100.00	WP_011227430
AELLADNN_1	662747	663949	STR_RS06895	Capsule	Immune modulation	99.42	100.00	WP_011227428
AELLADNN_1	659855	661003	STR_RS06910	Capsule	Immune modulation	99.22	100.00	WP_011227431
AELLADNN_1	937836	938626	cshA	Cell surface hydrophobicity proteins	Adherence	98.74	100.00	-
AELLADNN_1	1014661	1015396	STR_RS05250	Capsule	Immune modulation	98.50	98.13	WP_011227246
AELLADNN_1	1014661	1015401	STU_RS14635	Capsule	Immune modulation	98.25	100.00	WP_011226046
AELLADNN_1	1013219	1013950	STR_RS05260	Capsule	Immune modulation	98.22	100.00	WP_011227248
AELLADNN_1	665669	667414	STR_RS06885	Capsule	Immune modulation	98.22	100.00	WP_011227426
AELLADNN_1	1013219	1013950	STU_RS14645	Capsule	Immune modulation	98.09	100.00	WP_011226048
AELLADNN_1	844952	845713	srtA	Sortase A	Exoenzyme	97.64	100.00	-
AELLADNN_1	1011758	1013218	STU_RS14650	Capsule	Immune modulation	97.47	100.21	WP_011226049
AELLADNN_1	1011758	1013218	STR_RS05265	Capsule	Immune modulation	96.92	100.21	WP_041827043
AELLADNN_1	653708	654559	rfbD	Capsule	Immune modulation	96.83	100.00	WP_014621820
AELLADNN_1	1015458	1016821	STU_RS14630	Capsule	Immune modulation	94.94	99.71	WP_011226045
AELLADNN_1	1013959	1014651	STU_RS14640	Capsule	Immune modulation	89.90	100.00	WP_011226047
AELLADNN_1	1013959	1014651	STR_RS05255	Capsule	Immune modulation	89.61	100.00	WP_011227247
AELLADNN_1	1385522	1386825	eno	Streptococcal enolase	Exoenzyme	88.10	99.46	WP_000022832
AELLADNN_1	358647	359657	plr/gapA	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
AELLADNN_1	661941	662747	SGO_RS05020	Capsule	Immune modulation	82.90	100.00	WP_012000440
AELLADNN_1	1848219	1849502	tig/ropA	Trigger factor	Stress survival	80.73	99.77	WP_000107753
AELLADNN_1	881505	882551	rfbB	Capsule	Immune modulation	80.61	100.00	WP_009659423
AELLADNN_1	662747	663484	SGO_RS05025	Capsule	Immune modulation	84.96	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
AELLADNN_1	842265	841282	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
AELLADNN_1	1281456	1280383	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
AELLADNN_1	488817	486514	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
AELLADNN_1	1471884	1470916	spr0084	0.0	pathogenitcity related	92.88	98.48	AAK98888
AELLADNN_1	765509	764028	SPD_0310	0.0	UPF0371 protein SPD_0310	92.51	100.00	ABJ54480
AELLADNN_1	1249546	1247300	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
AELLADNN_1	85606	84608	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
AELLADNN_1	121721	120750	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
AELLADNN_1	605504	606655	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.32	99.74	AAL00050
AELLADNN_1	858465	860195	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
AELLADNN_1	161306	162784	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
AELLADNN_1	1386825	1385524	Eno	0.0	enolase	86.21	99.77	ABP90468
AELLADNN_1	176041	177933	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	85.74	98.90	ABP91129
AELLADNN_1	1244375	1246084	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
AELLADNN_1	316663	315653	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
AELLADNN_1	1093455	1094816	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
AELLADNN_1	264928	266820	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
AELLADNN_1	1401261	1400371	bglB	5.26e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
AELLADNN_1	1173094	1174032	SP_0676	8.22e-168	transcription facot	81.47	97.20	AAK74821
AELLADNN_1	1247108	1246251	metF	1.53e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
AELLADNN_1	974935	975639	covR	4.38e-131	responder protein	85.53	100.00	AAK71319
AELLADNN_1	790116	790787	rr01	3.01e-124	response regulator	83.93	99.56	CAB54566
AELLADNN_1	974935	975639	walR	1.85e-121	DNA-binding response regulator	82.13	100.00	ANC99504
AELLADNN_1	1696543	1695857	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
AELLADNN_1	1240420	1239752	CiaR	2.02e-117	two-component response regulator	84.31	99.55	AAK74935
AELLADNN_1	1240420	1239749	ciaR	1.99e-115	two component system response regulator	82.14	99.12	AIK75708
AELLADNN_1	84332	83958	SpxA2	1.15e-69	transcriptional regulator	91.20	94.70	ERL21461
AELLADNN_1	1082092	1081691	SpxA1	3.77e-66	transcriptional regulator	80.60	100.00	ERL19273
AELLADNN_1	1848061	1847561	RopE (M5005_Spy_1611)	4.93e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
AELLADNN_1	488817	486520	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
AELLADNN_1	280802	281821	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
AELLADNN_2	650	1594	rep37	945 / 945	100.00	HM050421

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
AELLADNN_1	0.0	1.0	0.0
AELLADNN_2	1.0	0.0	1.0
AELLADNN_3	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found