Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 2 | 8 | 8.77 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFLJGJKD_18 | 39118 | 40167 | clbA | ARO:3002814 | clbA | 98.57 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GFLJGJKD_18 | 39118 | 40153 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.80 | 98.67 | KR610408 |
| GFLJGJKD_34 | 263350 | 264726 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GFLJGJKD_18 | 39118 | 40161 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 98.85 | 99.71 | WP_012116915.1 |
| GFLJGJKD_34 | 263350 | 264720 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.65 | 99.78 | WP_003242953.1 |
| GFLJGJKD_23 | 177245 | 179839 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.57 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFLJGJKD_1 | 238130 | 239311 | capB | 0.0 | Capsule | Immune modulation | 82.12 | 99.66 | NP_391471 |
| GFLJGJKD_1 | 237666 | 238115 | capC | 1.65e-114 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFLJGJKD_9 | 31602 | 32738 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| GFLJGJKD_21 | 14817 | 13948 | pdxS | 5.86e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| GFLJGJKD_56 | 62163 | 61387 | CodY | 3.19e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| GFLJGJKD_16 | 9528 | 9920 | spxA1 | 1.04e-70 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| GFLJGJKD_18 | 24023 | 24220 | CspA | 2.33e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| GFLJGJKD_12 | 27164 | 27358 | CspD | 9.62e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| GFLJGJKD_10 | 14064 | 14258 | CspB | 1.22e-27 | cold shock protein | 84.61 | 98.48 | CAD00094 |
| GFLJGJKD_18 | 24023 | 24217 | CspR | 3.05e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| GFLJGJKD_4 | 9101 | 9973 | lipA (SAUSA300_0829) | 4.42e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFLJGJKD_1 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_2 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_3 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_4 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_5 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_6 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_7 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_8 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_9 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_10 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_11 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_12 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_13 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_14 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_15 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_16 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_17 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_18 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_19 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_20 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_21 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_22 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_23 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_24 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_25 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_26 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_27 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_28 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_30 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_31 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_32 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_33 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_34 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_35 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_38 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_39 | 1.0 | 0.0 | 1.0 |
| GFLJGJKD_40 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_45 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_56 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_67 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_73 | 0.0 | 1.0 | 0.0 |
| GFLJGJKD_74 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GFLJGJKD_8 | 39004 | 52709 | False | Siphoviridae | VOG7718, VOG3390, VOG5008, VOG8610, VOG7930, VOG0275, VOG9312 |
| GFLJGJKD_34 | 133357 | 166692 | False | Siphoviridae | VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG10500, VOG4609, VOG0327, VOG4548, VOG0198, VOG4581, VOG4544, VOG5051, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641, VOG4707 |
| GFLJGJKD_67 | 133901 | 138152 | False | Myoviridae | VOG4885, VOG5447, VOG4861, VOG0189 |
Phigaro v2.4.0 (Database: Jan. 2024 release)