safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	0	1	1.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
FOGLELEB_1	2933399	2932695	WalR	2.23e-111	transcriptional regulatory protein	83.40	100.00	EPH95667

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
FOGLELEB_1	0.0	1.0	0.0
FOGLELEB_2	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
FOGLELEB_1	560122	573131	False	Siphoviridae	VOG10059, VOG0181, VOG10867, VOG4799, VOG0189, VOG0982, VOG5280, VOG5317, VOG3307, VOG0198, VOG5088, VOG4570, VOG4570, VOG4549
FOGLELEB_1	900855	941307	False	Siphoviridae	VOG8520, VOG5353, VOG4552, VOG3307, VOG0181, VOG10867, VOG4967, VOG3642, VOG0321, VOG0322, VOG0226, VOG4799, VOG0025, VOG7735, VOG7018, VOG0536, VOG4567, VOG0152, VOG10904, VOG2338, VOG0198, VOG4570, VOG10904, VOG1345, VOG4841, VOG4581, VOG1886, VOG3644, VOG4556, VOG4568, VOG4553, VOG10303, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG2225, VOG6163, VOG4605, VOG4599, VOG6455, VOG7896, VOG0861, VOG7017, VOG0703, VOG4649
FOGLELEB_1	957344	993286	False	Siphoviridae	VOG0275, VOG6884, VOG6054, VOG4567, VOG3685, VOG7656, VOG4602, VOG6495, VOG5353, VOG4552, VOG0181, VOG4799, VOG0025, VOG7735, VOG0198, VOG3624, VOG5532, VOG4841, VOG0519, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG7880, VOG1898, VOG4545, VOG4633, VOG4763, VOG3498, VOG0648, VOG1637
FOGLELEB_1	1246570	1283144	False	Siphoviridae	VOG9667, VOG6495, VOG1305, VOG6179, VOG6495, VOG7236, VOG8241, VOG1309, VOG0186, VOG4566, VOG0226, VOG9860, VOG6623, VOG4693, VOG10869, VOG0198, VOG4892, VOG4570, VOG3699, VOG4544, VOG0720, VOG4564, VOG4555, VOG1329, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725, VOG0800, VOG4545, VOG4605, VOG4599, VOG6455, VOG7888, VOG0054, VOG4918
FOGLELEB_2	2	39192	False	Siphoviridae	VOG4553, VOG10303, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG2225, VOG6163, VOG4605, VOG4599, VOG6455, VOG7896, VOG0861, VOG7017, VOG0703, VOG4649, VOG9747, VOG0275, VOG0862, VOG8520, VOG5353, VOG4552, VOG3307, VOG0181, VOG10867, VOG4967, VOG3642, VOG0321, VOG0322, VOG0226, VOG4799, VOG0025, VOG7735, VOG7018, VOG0536, VOG4567, VOG0152, VOG10904, VOG2338, VOG0198, VOG4570, VOG10904, VOG1345, VOG4841