safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
15	0	0	15.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
IEDLPEAG_4	3002478	3004403	tet(Q)	ARO:3000191	tet(Q)	97.35	97.56	Z21523.1
IEDLPEAG_3	1762975	1763907	CfxA3	ARO:3003003	CfxA3	99.35	96.57	AF472622.2
IEDLPEAG_3	1490369	1491334	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
IEDLPEAG_3	1430292	1431224	CfxA2	ARO:3003002	CfxA2	99.03	96.57	AF118110.1
IEDLPEAG_3	1216020	1216985	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
IEDLPEAG_4	865404	866369	CfxA2	ARO:3003002	CfxA2	99.38	100.00	AF118110.1
IEDLPEAG_3	783109	785055	tet(Q)	ARO:3000191	tet(Q)	97.49	98.63	Z21523.1
IEDLPEAG_4	125952	126767	sul2	ARO:3000412	sul2	100.00	100.00	AY055428.1
IEDLPEAG_4	125088	125891	APH(3'')-Ib	ARO:3002639	APH(3'')-Ib	99.63	100.00	AF313472.2
IEDLPEAG_4	124252	125088	APH(6)-Id	ARO:3002660	APH(6)-Id	99.64	100.00	AF024602.1
IEDLPEAG_4	121538	123512	tet(Q)	ARO:3000191	tet(Q)	100.00	97.56	Z21523.1
IEDLPEAG_3	91878	92741	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
IEDLPEAG_3	89814	90635	ErmF	ARO:3000498	ErmF	99.62	102.63	M17124.1
IEDLPEAG_3	22646	23611	CfxA3	ARO:3003003	CfxA3	99.69	100.00	AF472622.2
IEDLPEAG_2	364	876	nimE	ARO:3007107	nimE	100.00	100.00	CP036548.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
IEDLPEAG_4	125088	125891	aph(3'')-Ib	Streptomycin	100.00	100.00	AF321551
IEDLPEAG_4	124252	125088	aph(6)-Id	Streptomycin	100.00	100.00	M28829
IEDLPEAG_2	364	876	nimE	Metronidazole	100.00	100.00	AM042593
IEDLPEAG_4	125952	126767	sul2	Sulfamethoxazole	100.00	100.00	AY034138
IEDLPEAG_4	121538	123463	tet(Q)	Doxycycline, Tetracycline, Minocycline	100.00	100.00	Z21523
IEDLPEAG_3	1216020	1216985	cfxA5	Unknown Beta-lactam	99.90	100.00	AY769934
IEDLPEAG_3	89835	90635	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.88	100.00	M17808
IEDLPEAG_4	865404	866369	cfxA3	Ampicillin	99.69	100.00	AF472622
IEDLPEAG_4	865404	866369	cfxA4	Unknown Beta-lactam	99.69	100.00	AY769933
IEDLPEAG_4	865404	866369	cfxA	Cefoxitin, Ampicillin	99.69	100.00	U38243

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
IEDLPEAG_3	91881	92741	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
IEDLPEAG_4	125091	125891	aph(3'')-Ib	aminoglycoside O-phosphotransferase APH(3'')-Ib	EXACTX	100.00	100.00	WP_001082319.1
IEDLPEAG_4	124255	125088	aph(6)-Id	aminoglycoside O-phosphotransferase APH(6)-Id	EXACTX	100.00	100.00	WP_000480968.1
IEDLPEAG_3	89835	90632	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
IEDLPEAG_2	364	873	nimE	5-nitroimidazole reductase NimE	EXACTX	100.00	100.00	WP_005812773.1
IEDLPEAG_4	125955	126767	sul2	sulfonamide-resistant dihydropteroate synthase Sul2	EXACTX	100.00	100.00	WP_001043260.1
IEDLPEAG_4	121541	123463	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	EXACTX	100.00	100.00	WP_002560998.1
IEDLPEAG_3	1216020	1216982	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
IEDLPEAG_3	1490372	1491334	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
IEDLPEAG_3	22646	23608	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.69	100.00	WP_004339683.1
IEDLPEAG_4	865404	866366	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	99.38	100.00	WP_004339683.1
IEDLPEAG_3	1762945	1763904	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	98.75	99.69	WP_004339683.1
IEDLPEAG_3	1430295	1431254	cfxA	CfxA family broad-spectrum class A beta-lactamase	BLASTX	98.44	99.69	WP_004339683.1
IEDLPEAG_3	783142	785055	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.65	99.53	WP_063856407.1
IEDLPEAG_4	3002478	3004400	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.35	100.00	WP_002560998.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
IEDLPEAG_1	1.0	0.0	1.0
IEDLPEAG_2	1.0	0.0	1.0
IEDLPEAG_3	0.0	1.0	0.0
IEDLPEAG_4	0.0	1.0	0.0
IEDLPEAG_5	0.0	1.0	0.0
IEDLPEAG_6	0.0	1.0	0.0
IEDLPEAG_7	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
IEDLPEAG_4	1680235	1687412	False	Siphoviridae	VOG7238,